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Preparation method of catharanthus roseus high-content anticancer alkaloid

A periwinkle, high-content technology, applied in the directions of alkaloids, alkaloids, chemical instruments and methods, etc., can solve the problems of not finding an effective or economical synthesis method, complex chemical structures, etc., and achieve high anti-cancer alkaloid content. Effect

Inactive Publication Date: 2012-05-23
廖龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Plant secondary metabolites have complex chemical structures, and no effective or economical synthesis methods have been found so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0005] Agrobacterium rhizogenes activated

[0006] ↓

[0007] Infection of periwinkle sterile explants with activated Agrobacterium rhizogenes

[0008] ↓

[0009] Separate the hairy roots growing from the infected site one by one

[0010] ↓

[0011] Hairy root clones were cultured in vitro

[0012] ↓

[0013] Determination of growth rate, alkaloid content and composition analysis and comparison with the original plant

[0014] ↓

[0015] Obtain excellent hairy root clones with high content of anticancer alkaloids

[0016] ↓

[0017] Establishment of excellent clone culture system (fermenter)

[0018] ↓

[0019] Extraction, separation and purification of alkaloids

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PUM

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Abstract

The invention relates to a preparation method of catharanthus roseus high-content anticancer alkaloid. According to the invention, hairy roots obtained through agrobacterium rhizogenes mediation are subject to several times of subculture; an excellent clone with high growth speed and high and stable alkaloid content are screened according to the growth rate and the alkaloid content; the hairy root excellent clone is cultured by using a miniature fermenting machine (10-30L); through adjustments upon conditions such as medium components, ventilation status, illumination, temperature, exogenous hormone and culture mode, technology indexes and process flow of hairy root culturing are obtained; a hairy root clonal propagation system is established, and alkaloid is extracted, separated and purified from the hairy root.

Description

【Technical field】 [0001] The invention relates to a method for preparing a high-content anticancer alkaloid of periwinkle. Vinca is a medicinal plant of the family Apocynaceae, and natural plants contain more than 100 kinds of alkaloids, of which the anticancer active ingredients vinblastine and vincristine are The content is very small, only a few parts per 100,000 to a few parts per million. Since vinblastine and vincristine are effective drugs for treating leukemia, lymphosarcoma, choriocarcinoma and other tumors, their value is very expensive. Applying hairy root transformation technology to induce periwinkle hairy roots, screening and establishing a fast-growing, high and stable hairy root clonal propagation system, will be the industrialized production and industrialization of periwinkle anticancer alkaloids Develop an effective path. 【Background technique】 [0002] Plant secondary metabolites have complex chemical structures, and no effective or economical synthesis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D519/04C07G5/00C12N5/04
Inventor 廖龙
Owner 廖龙
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