Valine purification method

Abstract

The invention relates to a valine purification method, which comprises the following steps: A. adding flocculating agents into valine fermentation liquid for fast precipitating solid impurities, and obtaining primary filter liquid through filtering supernatant; B. adding activated carbon into the primary filter liquid obtained in the step A, wherein the added activated carbon accounts for 1+ / -0.5weight percent of the filter liquid, carrying out stirring and decoloration at 60 + / - 5 DEG C, and then, obtaining secondary filter liquid through filtration; C. using an industrial chromatographic column for separating inorganic salt and heteroacid, and then, using a sodium filter membrane for removing pigment and small molecular impurities to obtain a clear and colorless valine solution; D. concentrating the filter liquid for crystallization to obtain crude valine products; and E. shaking a transmission belt and raising the temperature to 220 + / - 10 DEG C, and carrying out sublimation to remove alanine and isoleucine to obtain pharmaceutical grade competitive valine products. The method has the characteristics of simple operation, good selectivity, cleanness and environment protection, so the yield and the quality of the valine are obviously improved.

Description

technical field [0001] The invention relates to a method for extracting and purifying valine from fermentation broth, especially a method for extracting pharmaceutical grade valine. Background technique [0002] The production methods of L-valine include extraction method, synthesis method, fermentation method and so on. The content of L-valine in animal blood meal, silkworm chrysalis and hair hydrolyzate is relatively high. Ion exchange technology is used to separate valine from mixed amino acids. The separation efficiency is high, the extraction operation is simple, and the production cycle is short, but the cost is high and it is not suitable for Modern industrial production. The chemical synthesis method uses isobutyraldehyde as raw material, reacts with ammonia and hydrocyanic acid to generate cyanamide, then hydrolyzes to obtain DL-valine, and obtains L-valine after resolution. The chemical synthesis method has high production cost, complex reaction, many steps and m...

AI Technical Summary

Problems solved by technology

This method is very complicated, and it is difficult to separate valine from leucine and isoleucine, and there are problems such as time-consuming, low efficiency, and high content of impurity amino acids; 2, adding hydrochloric acid to the aqueous solution containing valine In order to generate and precipitate valine hydrochloride crystals, repeat the operation [Japanese Laid-Open Patent Application (Kokai) No.16, 450 / 1981]

The yield of valine obtained by this method is reduced

3. Purify valine by reacting it with precipitating agents such as tetrachlorophthalic acid, sulfoisophthalic acid, sulfonine, etc., which selectively form compounds with valine (Japanese Patent Pubication No.25059 / 1967) the precipitating agent used in this method is expensive and industrially difficult to obtain, the resulting compound has high solubility, it is difficult to reclaim valine with high yield, and the separation of valine from the compound is relatively complicated

Chinese patent 200710021935 removes the ion exchange process and directly adopts the method of concentration and crystallization. Although the waste water discharge is greatly reduced and the yield is improved, the product has low purity and contains many impurities.