Yolk antibody of anti SARS coronavirus and its preparation method and liquid preparation

A coronavirus and egg yolk antibody technology, applied in the field of egg yolk antibodies, can solve problems such as inability to disinfect and protect the human body, achieve the effects of preventing severe acute respiratory syndrome, high extraction efficiency, and easy industrialization

Inactive Publication Date: 2004-12-22
BIOINFORBODY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commonly used disinfectants for the prevention of SARS mainly include chlorine-containing disinfectants and peracetic acid disinfectants, as well as the newly developed new type of bromine-containing disinfectants. Although these disinfectants can effectively kill coronaviruses, they are also harmful to The human...

Method used

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  • Yolk antibody of anti SARS coronavirus and its preparation method and liquid preparation
  • Yolk antibody of anti SARS coronavirus and its preparation method and liquid preparation
  • Yolk antibody of anti SARS coronavirus and its preparation method and liquid preparation

Examples

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Effect test

Embodiment 1

[0079] Anti-SARS coronavirus yolk antibody of the present invention, it detects according to non-denaturing polypropylene gel electrophoresis (resolving gel concentration is 6.0%), after Coomassie brilliant blue R250 staining, Atlas It presents a single band, and Western Blotting detection can find a single specific enzyme stained band at a molecular weight of about 170-190KD (such as figure 1 ); or detect according to deformed polypropylene gel electrophoresis (resolving gel concentration is 6.0%), after Coomassie Brilliant Blue R250 staining, Atlas Presents two bands, and Western Blotting detection can find two specific enzyme-stained bands at molecular weights of about 65KD and 25KD (such as figure 2 ); the protein concentration is determined to be 0.5-20 mg / ml by ultraviolet spectrophotometry; the titer is determined to be 1:50-1:100000 by enzyme-linked immunosorbent assay.

[0080] The preparation method of the yolk antibody of described anti-SARS coronavirus adopts ...

Embodiment 2

[0090] Anti-SARS coronavirus yolk antibody of the present invention, it detects according to non-denaturing polypropylene gel electrophoresis (resolving gel concentration is 6.0%), after Coomassie brilliant blue R250 staining, Atlas It presents a single band, and Western Blotting detection can find a single specific enzyme stained band at a molecular weight of about 170-190KD (such as figure 1 ); or detect according to deformed polypropylene gel electrophoresis (resolving gel concentration is 6.0%), after Coomassie Brilliant Blue R250 staining, Atlas Presents two bands, and Western Blotting detection can find two specific enzyme-stained bands at molecular weights of about 65KD and 25KD (such as figure 2 ); the protein concentration is determined to be 0.5-20 mg / ml by ultraviolet spectrophotometry; the titer is determined to be 1:50-1:100000 by enzyme-linked immunosorbent assay.

[0091] The preparation method of the yolk antibody of described anti-SARS coronavirus is to f...

Embodiment 3

[0126] Anti-SARS coronavirus yolk antibody of the present invention, it detects according to non-denaturing polypropylene gel electrophoresis (resolving gel concentration is 6.0%), after Coomassie brilliant blue R250 staining, Atlas It presents a single band, and Western Blotting detection can find a single specific enzyme stained band at a molecular weight of about 170-190KD (such as figure 1 ); or detect according to deformed polypropylene gel electrophoresis (resolving gel concentration is 6.0%), after Coomassie Brilliant Blue R250 staining, Atlas Presents two bands, and Western Blotting detection can find two specific enzyme-stained bands at molecular weights of about 65KD and 25KD (such as figure 2 ); the protein concentration is determined to be 0.5-20 mg / ml by ultraviolet spectrophotometry; the titer is determined to be 1:50-1:100000 by enzyme-linked immunosorbent assay.

[0127] The preparation method of the yolk antibody of described anti-SARS coronavirus is to f...

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Abstract

A yolk antibody for SARS coronavirus is prepared through injecting the antigen, which may be recombinant genetic protein S, M, or E,or the antigen epitope for said protein able to represent SARS coronavirus, or the synthetic polypeptide of said protein, etc, in health hen for primary immunizing, booster immunizing, collecting its egg, extracting yolk, and extracting the yolk antibody from it. It can also be prepared to become liquid preparation. It can be used to prevent SARS.

Description

technical field [0001] The invention relates to an anti-SARS coronavirus yolk antibody, a preparation method and a liquid preparation thereof. Background technique [0002] The disease characterized by fever and lung infection that began to appear in Guangdong in November 2002 is internationally known as severe acute respiratory syndrome (Severe Acute Respiratory Syndrome, referred to as SARS). As of May 26, 2003, SARS cases have been found in 31 countries around the world, and the cumulative number of cases worldwide has reached 8,202. The disease is endemic in 25 provinces, municipalities and autonomous regions in my country, and the cumulative number of cases has reached 5,316. The prevalence of the disease has had a very serious impact on the economic and social stability of various countries. [0003] The SARS virus belongs to the Coronaviridae family, and the virus particles are mostly round, with a capsule, and there are coronal-arranged filament...

Claims

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Application Information

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IPC IPC(8): C07K16/02C07K16/08
Inventor 叶志海丁波王文夏鲁全
Owner BIOINFORBODY
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