The invention relates to the technical field of in-vitro
diagnostic reagent preparation, in particular to a high-stability
protein S activity determination kit and a preparation method thereof, and the high-stability
protein S activity determination kit comprises an activator, a substrate, a stop buffer and a standard substance; the activating agent contains
snake venom protease,
trehalose and the like and can activate
protein S; the substrate contains a
chromogenic substrate S-2366 and the like for
chromogenic reaction; the stop solution contains glacial
acetic acid and the like and is used for stopping reaction and stably developing; the standard substance contains recombinant
protein S and the like for calibration; special components are added into the components to improve the stability, for example,
zinc and
magnesium ions are added into an activator to enhance the
enzyme activity, and
arginine is used as a substrate to inhibit
hydrolysis; the components of the kit are independently packaged, and the pH and performance stable period at 2-8 DEG C is as long as 6 months. Through innovative formula design, the
reagent stability and the detection accuracy are remarkably improved; special components enhance
enzyme and substrate stability and reduce background interference; a
standard product is optimized to improve the calibration precision; the preparation process guarantees batch-to-batch consistency, and is simple and convenient to operate, high in
interference resistance and suitable for various clinical scenes.