The use of protein S fusion for protein solubilization

A protein and target protein technology, which is used in the expression of enhanced stability/folded protein fusion, hybrid peptides, and introduction of foreign genetic material using vectors, which can solve problems such as widening, inability to purify proteins, and low solubility.

Inactive Publication Date: 2009-04-08
UNIV OF MEDICINE & DENTISTRY OF NEW JERSEY
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  • Abstract
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  • Application Information

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Problems solved by technology

[0008] Disadvantages of existing fusion-tagging systems include the low solubility of the tagged fusion protein and the inability to purify the tagged protein under conditions common in many protein purification protocols
In addition, the existing fusion tags are difficult to use in the analysis of protein structure
For example, His tags cannot be used in experiments designed for NMR structural studies because Ni-NTA resins cannot be used for NMR spectroscopy because Ni ++ Paramagnetic effects of ions cause peak broadening and interfere with data collection

Method used

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  • The use of protein S fusion for protein solubilization
  • The use of protein S fusion for protein solubilization
  • The use of protein S fusion for protein solubilization

Examples

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Embodiment

[0080] The following examples are given to provide those of ordinary skill in the art with a complete illustration and description of how to make and use the invention, and are not intended to limit the scope of the invention as the inventors regard it, nor are they intended to represent the following Tests are all or the only tests that can be performed. Efforts have been made to ensure accuracy with respect to numbers used (eg amounts, temperature, etc.), but some experimental errors and deviations should be accounted for. Unless indicated differently, parts are parts by weight, molecular weight is weight average molecular weight, temperature is in degrees Centigrade, and pressure is at or near atmospheric.

[0081] For numerical ranges given herein, it is understood that 1 / 10 of the unit of the lower limit (unless the context indicates otherwise), each intervening value between the upper and lower limits of that range, and any other Any specified or intervening values ​​wi...

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Abstract

The invention provides vectors containing a multiple cloning site comprising a PrS tag or a PrS2 tag from Myxococcus xanthus. Methods are provided for enhancing solubility of a target protein using Protein S tagged target fusion proteins.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Application No. 60 / 783,998, filed March 20, 2006, by Inouye et al., entitled "The Use of Protein S Fusion for Protein Solubilization." This application is hereby incorporated by reference in its entirety. field of invention [0003] The present invention relates to systems for increasing the production and solubility of proteins. [0004] Statement under C.F.R.1.821(f) [0005] Pursuant to §1.821(f), the contents of the attached paper copy and the attached computer-readable copy "18622-12umdnj.st25.txt" of the sequence listing filed pursuant to 37 C.F.R. §1.821(c) and (e), respectively is consistent. Background of the invention [0006] Protein expression is a major challenge in medicine and biotechnology because of the large number of proteins that become insoluble when expressed in a number of different conventional expression systems. Many protocols have been de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/64C12P21/04C07H21/04C07K19/00
CPCC07K2319/35C07K14/195C12N15/64C07K19/00C12N15/09C12P21/02
Inventor M·井上T·吉田
Owner UNIV OF MEDICINE & DENTISTRY OF NEW JERSEY
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