Method for separating and purifying high-purity activated clotting seventh factors from cell culture solution or plasma components

A cell culture, separation and purification technology, applied in the field of separation and purification of high-purity activated blood coagulation factor seven, can solve problems such as adverse reactions, achieve good safety, simple and efficient thrombin activation process, and improve comprehensive utilization
CN102161701AInactive Publication Date: 2011-08-24SYNDEGEN SHANGHAI BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SYNDEGEN SHANGHAI BIOTECH
Publication Date
2011-08-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for separating and purifying high-purity activated clotting seventh factors from a cell culture solution or plasma components, successively comprising the following steps: carrying out affinity chromatography purification on raw materials; collecting an eluent containing a clotting seventh factor; adding thrombin and magnesium ions to activate the clotting seventh factor; and finally, condensing an activated product, and carrying out virus inactivation to obtain a final product. The clotting seventh factor can be purified in one step with the affinity chromatography method, the thrombin activation process is simple and efficient, the seventh factor can be purified and separated from the cell culture solution or plasma components, the multipurpose utilization ratio of the plasma is greatly improved, and a safe and efficient high-purity seventh factor protein drug can be provided.
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Description

technical field

[0001] The invention relates to the technical field of biomedicine, in particular to a method for separating and purifying high-purity activated blood coagulation factor seven from cell culture fluid or plasma components. Background technique

[0002] Coagulation factor VII (FVII) is a vitamin K-dependent glycoprotein, synthesized in the liver, and exists in a very low concentration (0.5mg / L) in the blood, and mainly exists in the form of an inactive single-chain zymogen, only 1% are activated. Factor seven zymogens can be activated by activated coagulation factors twelfth, ten, nine, seven, or thrombin to form activated factor seven (FVIIa). Factor seven is the zymogen necessary to start the extrinsic coagulation pathway, and its activation is the beginning of the extrinsic coagulation pathway. Activated factor VII itself has very low proteolytic activity, while tissue factor (TF) is a highly affinity receptor for activated factor VII. Once activated facto...

Claims

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