Bacillus subtilis and construction method and application thereof

Abstract

The invention belongs to the field of microorganisms and discloses bacillus subtilis and a construction method and application thereof. At least one of purDP116L, purRA65D or guaBG279R in bacillus subtilis strains is in site mutation. B. subtilis A1, A2 and A3 strains are obtained respectively through single-site mutation of purDP116L, purRA65D or guaBG279R. Three single-site mutation plasmids sequentially convert a B. subtilis 168 (Delta upp) strain to obtain a B. subtilis A5 strain with three sites all in site mutation. The bacillus subtilis is an adenosine high-yield strain, adenosine can be accumulated effectively, yield of adenosine is increased, and a foundation is laid for industrialized production of adenosine.

Description

Technical field

[0001] The invention belongs to the field of microorganisms, and specifically relates to a Bacillus subtilis and a construction method and application thereof, in particular to a Bacillus subtilis that produces adenosine, and a construction method and application thereof. Background technique

[0002] Adenosine is adenosine, its chemical name is 6-amino-9-β-D-ribofuranosyl-9-hydropurine. It is the product of adenine nucleotide dephosphorylation and is an important nucleotide derivative Things. Adenosine is an endogenous nucleoside that is distributed throughout human cells. It can directly enter the myocardium to generate adenylate through phosphorylation, and participate in myocardial energy metabolism. At the same time, it also participates in expanding coronary blood vessels and increasing blood flow. Adenosine has physiological effects on the cardiovascular system and many other systems and tissues of the body. In addition to being used as a special medicine...

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