Cupriavidus, cupriavidus preparation, and application of cupriavidus preparation in heavy metal contaminated soil remediation
A technology of contaminated soil and copper greedy bacteria, which is applied in the restoration of contaminated soil, bacteria, and microbial-based methods to achieve good tolerance, achieve adsorption and accumulation, and reduce the effect of cadmium content
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0028] Example 1
[0029] Isolation, screening and verification of cadmium tolerance of cadmium-resistant bacterial strain Cd02
[0030]The strain was isolated, screened and purified from cadmium-contaminated soil samples. The specific method is as follows: Take 1g of the above soil sample, add 100mL of sterile water, shake it in a constant temperature shaker with a rotation speed of 150rpm for 24h, let it stand for 30min, take 1mL of the supernatant and spread it on 100mg / L cadmium-containing solid LB culture In the medium, when there are bacteria growing around, pick the bacteria and streak in the solid LB medium, put them in a constant temperature incubator for 24 hours, observe the colony shape, and streak the grown single colony to the fresh LB solid Cultivate it on a plate to obtain a pure strain named Cd02. Finally, the Cd02 strain was streaked on an inclined test tube, placed in a constant temperature incubator at 30°C for 24 hours, and then stored in a refrigerator ...
Example Embodiment
[0039] Example 2
[0040] Changes in pH of the culture medium and its ability to produce alkali during the culture of Cd02.
[0041] Inoculate 2% (thalline concentration 0.93~1.86g / L) Cd02 bacterial suspension in logarithmic phase of growth to nutrient solution (every liter of culture solution contains: 0.5g K 2 HPO 4 , 1g NH 4 Cl, 1g Na 2 SO 4 , 0.1g CaCl 2 2H 2 O, 2g MgSO 4 ·7H 2 (2, 2g sodium lactate), the cadmium ion concentration is respectively set to 0mg / L, 2mg / L, 4mg / L, 6mg / L, puts the vibration culture under 30 ℃ of rotating speed 150rpm, respectively in 24h, 48h, 72h, 96h, 120h, Samples were taken at 144h, and the pH of the culture solution was measured with a pH meter. Each group has 3 repetitions.
[0042] Experimental results such as Figure 5 As shown in (A), the pH of the cadmium-free culture solution increases with time, and reaches 8.6 in the later stage of cultivation; in the cadmium-containing culture solution, the pH of the culture solution also ...
Example Embodiment
[0044] Example 3
[0045] Adsorption and accumulation of heavy metal cadmium ions by Copper greedy bacteria Cd02.
[0046] Inoculate 2% (0.93~1.86g / L) Cd02 bacterial suspension in logarithmic phase of growth to cadmium-containing culture solution (every liter of culture solution contains: 0.5g K 2 HPO 4 , 1g NH 4 Cl, 1g Na 2 SO 4 , 0.1g CaCl 2 2H 2 O, 2g MgSO 4 ·7H 2 (2, 2g sodium lactate), the concentration of cadmium ions is set to 2mg / L, 4mg / L, 6mg / L, 8mg / L respectively, and the rotation speed is 150rpm at 30°C for shaking culture, respectively in 24h, 48h, 72h, 96h, 120h, Take samples at 144 hours, and measure the cadmium ion content in the solution and the cadmium ion content in the bacterial cell wall and the cells with an atomic absorption spectrophotometer (flame photometry). Each group has 3 repetitions.
[0047] Experimental results such as Image 6 As shown, during the culture period, when the pH value increased from 7.5 to 8.6, due to H + The reduction ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap