This invention presents a
Raman spectroscopy-based method for evaluating low-
dose irradiation in organoids, belonging to the field of
organoid radiobiological effect evaluation /
Raman spectroscopy in vitro biological detection. Given that low-
dose irradiation induces relatively small biochemical changes in organoids, and that organoids themselves undergo natural maturation / metabolic drift over culture time, the
irradiation effect is easily masked by "
time drift." This invention constructs a time-point
matched control correction evaluation framework: establishing a control baseline at each time point and extracting the net irradiation effect under this baseline, thereby achieving comparability across time points and batches. It also enables low-
dose sensitive differential readout and four-quadrant
genotyping output, which can be used to evaluate the differences in
nucleic acid-related responses caused by different doses of irradiation at different time points, and to compare the differences in response intensity and pattern under intervention conditions such as irradiation
sensitization / protection, providing a basis for subsequent screening and stratified evaluation.