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Process of extracting pueraria polysaccharide

A kudzu root polysaccharide and extraction technology, which is applied in the field of kudzu root soluble polysaccharide extraction, can solve the problems that the product is prone to browning, the extraction rate is not fully considered, and the product color, purity and storage time are affected.

Inactive Publication Date: 2007-08-22
宋桃森
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AI Technical Summary

Problems solved by technology

Due to its emphasis on comprehensive technical requirements for synchronous extraction of multiple components, the extraction rate of a single component of Pueraria polysaccharides has not been fully considered, and the extraction rate and purity of Pueraria polysaccharides may be further improved
Moreover, the existing kudzu root active ingredient extraction technology including the above-mentioned technology has an important defect, that is, the extracted product is prone to browning during the extraction process and storage process, which affects the color, purity and storage time of the product.

Method used

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Experimental program
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Embodiment Construction

[0016] Pueraria polysaccharide extraction process, the specific steps are:

[0017] (1), cleaning: get 100 kudzu root, clean kudzu root, cut into sections, soak in water for 20 hours;

[0018] (2), refining: take out the kudzu root, add water to refine the slurry, the ratio of material to water is 1:6, and treat with ultraviolet light for 10-20 minutes;

[0019] (3), enzymolysis and ultrasonic treatment: prepare enzyme preparation by the weight ratio of LS-1 composite cellulase: papain=5: 1, add enzyme preparation in slurry and carry out enzymolysis reaction, and carry out ultrasonic treatment simultaneously, The dosage of the enzyme preparation is 0.04 g / 100 g of kudzu root slurry, and the enzymatic hydrolysis conditions are: time 1.0 hour, temperature 45°C, pH 6-6.5, ultrasonic frequency 12kHZ;

[0020] (4), 120 mesh filter, remove fiber;

[0021] (5) The filtrate is squeezed into a centrifuge, and the centrifugal separation is performed for 10 minutes at a rotating speed ...

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Abstract

The process of extracting pueraria polysaccharide includes the following steps: milling slurry with kudzu vine root, adding cellulase and protease into the slurry for enzymolysis and simultaneous low strength ultrasonic treatment, adding vitamin C and xylose for protecting color, filtering to eliminate fiber, centrifugally separating the filtrate to eliminate starch, vacuum concentrating the centrifugate, adding alcohol before further centrifugally separating to obtain wet pueraria polysaccharide, and drying to obtain pueraria polysaccharide product. The present invention has raised enzyme activity and substrate converting rate, raised extraction rate of polysaccharide and flavone, effective prevention of browning, and natural color of the product.

Description

technical field [0001] The invention relates to the extraction of plant effective components, in particular, it provides an extraction method of kudzu root soluble polysaccharide. Background technique [0002] Pueraria lobata is the root tuber of perennial deciduous vines or Pueraria lobata. Pueraria is a traditional Chinese medicine, first planted in "Shen Nong's Materia Medica". Li Shizhen's "Compendium of Materia Medica" records that Pueraria is sweet, pungent, flat, and non-toxic. It has obvious curative effects on wind-fire toothache, sore throat, hot cough, high fever, headache, obesity, etc. It has special effects on preventing high blood pressure, high blood fat, and coronary heart disease. Modern science has proved that high-quality starch, active polysaccharides and isoflavones (isoflavoness) are the main components contained in kudzu root, and it also contains crude fiber, protein, vitamins, minerals, puerosides, triterpenoid saponins ( triterpenoid saponin), al...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 宋桃森
Owner 宋桃森
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