The invention discloses an extraction and purification method for a fresh fagaceae
plant sample and a fagaceae
plant specimen. According to the extraction method, before nuclear
lysis, a special rinsing solution containing
ascorbic acid, Triton-X 100 and the like is added, so that not only can impurities such as
polysaccharide and
polyphenol be effectively removed to make obtained DNAs not viscousany longer, but also
pigment pollution can be effectively removed to endow the extracted DNAs with high purity and high yield; compared with the step sequence of removing the impurities after the nuclear
lysis, the step sequence adopted by the extraction method provided by the invention has the advantages of a better effect, and the extraction method is applicable to a later molecular experiment;according to the purification method, a self-prepared
silica gel adsorption solution prepared from
silica gel powder is used, and according to the purification method, the DNAs can be effectively purified, the high-purity DNAs can be obtained, and downstream PCR and
enzyme digestion experiments can be met. According to the extraction and purification method, difficulties of the conventional CTABmethod and the conventional kit in fagaceae
plant extraction are overcome; the extraction and purification method can be used for extracting DNAs of fresh fagaceae leaves, dry
silica gel samples and dried plant specimens with relatively long preservation time and is applied to high-
throughput sequencing.