Method for preparing cured specimens of citrus leaf disease
A preparation method and a specimen technology are applied in the field of preparation of citrus leaf disease cured specimens, which can solve the problems of being susceptible to damp and mildew, difficult to grasp the chemical change law, and losing the use value, so as to achieve convenient repeated use, convenient observation and placement, Long-lasting effects
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Embodiment 1
[0036] The preparation method of citrus leaf disease curing specimen comprises the following steps:
[0037] 1) Collection of specimens: typical, complete and representative citrus diseased leaves, soaked and disinfected in 70% alcohol for 5 minutes;
[0038] 2) Preparation of the color-preserving solution: adding solid copper oxide into 50% glacial acetic acid solution to obtain a saturated solution of copper acetate, diluting it 1.5 times with sterile water to obtain the color-preserving solution;
[0039] 3) Color retention: After heating the color retention solution to 85°C, add the sample collected in step 1) and perform color retention treatment for 10 minutes. After the leaves change from green to yellow-green or brown to green, take out the sample and use Rinse with bacterial water, then place in color-preserving solution and soak at room temperature for 2 days;
[0040] 4) Apply colorless nail polish evenly on the surface of the specimen after pressing and drying the...
Embodiment 2
[0044] The preparation method of citrus leaf disease curing specimen comprises the following steps:
[0045] 1) Collection of specimens: typical, complete and representative citrus diseased leaves, soaked and disinfected in 70% alcohol for 5 minutes;
[0046] 2) Preparation of the color-preserving solution: adding solid copper oxide into 50% glacial acetic acid solution to obtain a saturated solution of copper acetate, diluting it twice with sterile water to obtain the color-preserving solution;
[0047] 3) Color retention: After heating the color retention solution to 88°C, add the sample collected in step 1) and perform color retention treatment for 10 minutes. After the leaves change from green to yellow-green or brown to green, take out the sample and use Rinse with bacterial water, then place in color-preserving solution and soak at room temperature for 2 days;
[0048] 4) Apply colorless nail polish evenly on the surface of the specimen after pressing and drying the spe...
Embodiment 3
[0052]The preparation method of citrus leaf disease curing specimen comprises the following steps:
[0053] 1) Collection of specimens: typical, complete and representative citrus diseased leaves, soaked and disinfected in 70% alcohol for 5 minutes;
[0054] 2) Preparation of color-preserving solution: adding solid copper oxide into 50% glacial acetic acid solution to obtain a saturated solution of copper acetate, diluting it with sterile water to obtain a color-preserving solution;
[0055] 3) Color retention: After heating the color retention solution to 80°C, add the sample collected in step 1) and perform color retention treatment for 20 minutes. After the leaves change from green to yellow-green or brown to green, take out the sample and use Rinse with bacterial water, then place in color-preserving solution and soak at room temperature for 2 days;
[0056] 4) Apply colorless nail polish evenly on the surface of the specimen after pressing and drying the specimen after c...
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