Method for preparing cured specimens of citrus leaf disease

A preparation method and a specimen technology are applied in the field of preparation of citrus leaf disease cured specimens, which can solve the problems of being susceptible to damp and mildew, difficult to grasp the chemical change law, and losing the use value, so as to achieve convenient repeated use, convenient observation and placement, Long-lasting effects

Inactive Publication Date: 2018-12-25
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The original color and undisturbed specimens of plants not only play an important role in teaching and scientific research, but also have important value in specimen display, display and artistic appreciation. Due to the complex chemical components contained in plants, it is difficult to grasp the law of chemical changes. The production of primary color herbarium poses great difficulties
[0003] At present, there are the following main problems in the traditional production of primary color specimens of plants: Some methods of preserving plant disease specimens are prone to damp and moldy, and are also easy to be eaten by insects and lose their use value.
[0005] At present, there is no report on the curing specimens of citrus leaf diseases, so developing a method for making cured specimens of citrus leaf diseases is of great significance for the teaching and scientific research of citrus diseases

Method used

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  • Method for preparing cured specimens of citrus leaf disease
  • Method for preparing cured specimens of citrus leaf disease
  • Method for preparing cured specimens of citrus leaf disease

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The preparation method of citrus leaf disease curing specimen comprises the following steps:

[0037] 1) Collection of specimens: typical, complete and representative citrus diseased leaves, soaked and disinfected in 70% alcohol for 5 minutes;

[0038] 2) Preparation of the color-preserving solution: adding solid copper oxide into 50% glacial acetic acid solution to obtain a saturated solution of copper acetate, diluting it 1.5 times with sterile water to obtain the color-preserving solution;

[0039] 3) Color retention: After heating the color retention solution to 85°C, add the sample collected in step 1) and perform color retention treatment for 10 minutes. After the leaves change from green to yellow-green or brown to green, take out the sample and use Rinse with bacterial water, then place in color-preserving solution and soak at room temperature for 2 days;

[0040] 4) Apply colorless nail polish evenly on the surface of the specimen after pressing and drying the...

Embodiment 2

[0044] The preparation method of citrus leaf disease curing specimen comprises the following steps:

[0045] 1) Collection of specimens: typical, complete and representative citrus diseased leaves, soaked and disinfected in 70% alcohol for 5 minutes;

[0046] 2) Preparation of the color-preserving solution: adding solid copper oxide into 50% glacial acetic acid solution to obtain a saturated solution of copper acetate, diluting it twice with sterile water to obtain the color-preserving solution;

[0047] 3) Color retention: After heating the color retention solution to 88°C, add the sample collected in step 1) and perform color retention treatment for 10 minutes. After the leaves change from green to yellow-green or brown to green, take out the sample and use Rinse with bacterial water, then place in color-preserving solution and soak at room temperature for 2 days;

[0048] 4) Apply colorless nail polish evenly on the surface of the specimen after pressing and drying the spe...

Embodiment 3

[0052]The preparation method of citrus leaf disease curing specimen comprises the following steps:

[0053] 1) Collection of specimens: typical, complete and representative citrus diseased leaves, soaked and disinfected in 70% alcohol for 5 minutes;

[0054] 2) Preparation of color-preserving solution: adding solid copper oxide into 50% glacial acetic acid solution to obtain a saturated solution of copper acetate, diluting it with sterile water to obtain a color-preserving solution;

[0055] 3) Color retention: After heating the color retention solution to 80°C, add the sample collected in step 1) and perform color retention treatment for 20 minutes. After the leaves change from green to yellow-green or brown to green, take out the sample and use Rinse with bacterial water, then place in color-preserving solution and soak at room temperature for 2 days;

[0056] 4) Apply colorless nail polish evenly on the surface of the specimen after pressing and drying the specimen after c...

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Abstract

The invention belongs to the field of plant specimen preparation, and particularly relates to a method for preparing cured specimens of a citrus leaf disease. The preparation method provided by the invention comprises the following steps: collecting specimens; adding solid copper oxide to 50% glacial acetic acid solution to obtain a copper acetate saturated solution, and diluting the solution withsterile water for 1 to 2.5 times to obtain a color retention liquid; adding the color retention liquid into the collected specimens after being heated for color retention treatment, and taking out the specimens after the leaves are changed from green to yellow-green or brown and to green again; evenly coating colorless nail polish on the surfaces of the specimens after the specimens through the color retention are pressed and dried, placing the specimens in a neat by resins after drying for embossing, and obtaining cured specimens of the citrus leaf disease after drying. The cured specimen ofthe citrus leaf disease prepared by the preparation method provided by the invention can be preserved for a long time, and can completely and truly reflect the characteristics of the citrus leaf disease, which is convenient for repeated use and observation, and has important significance in teaching and research.

Description

technical field [0001] The invention belongs to the field of making plant specimens, and in particular relates to a method for preparing cured specimens of citrus leaf diseases. Background technique [0002] The original color and undisturbed specimens of plants not only play an important role in teaching and scientific research, but also have important value in specimen display, display and artistic appreciation. Due to the complex chemical components contained in plants, it is difficult to grasp the law of chemical changes. The production of primary color herbarium poses great difficulties. [0003] At present, the following main problems exist in the traditional production of natural color specimens of plants: some methods of preserving specimens of plant diseases are prone to moisture and mildew, and are also easy to be eaten by insects and lose their use value. In addition, during the observation and use of most specimens, due to the sealing limitations of specimen box...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/30G01N2001/305
Inventor 姚廷山肖田胡军华刘浩强
Owner SOUTHWEST UNIVERSITY
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