56 results about "Specimen observation" patented technology

A bio electron microscope and an observation method which can observe a bio specimen by low damage and high contrast to perform high-accuracy image analysis, and conduct high-throughput specimen preparation. 1) A specimen is observed at an accelerating voltage 1.2 to 4.2 times a critical electron accelerating voltage possible to transmit a specimen obtained under predetermined conditions. 2) An electron energy filter of small and simplified construction is provided between the specimen and an electron detector for imaging by the electron beam in a specified energy region of the electron beams transmitting the specimen. 3) Similarity between an observed image such as virus or protein in the specimen and a reference image such as known virus or protein is subjected to quantitative analysis by image processing. 4) A preparation protocol of the bio specimen is made into a chip using an MEMS technique, which is then mounted on a specimen stage part of an electron microscope to conduct specimen introduction, preparation and transfer onto a specimen holder.

A microscope system includes an acquisition unit that obtains a specimen image acquired by capturing the specimen stained by an element identification dye that visualizes a predetermined cell constituent element and a molecule target dye that visualizes a predetermined target molecule by using a microscope; a dye amount unit that obtains dye amounts of the element identification dye and the molecule target dye that stain corresponding positions on the specimen for each pixel of the image; an element area identification unit that identifies an area of the cell constituent element based on the dye amount of the element identification dye; a condition setting unit that sets the presence or absence of the predetermined target molecule on the cell constituent element as a condition; and an extraction unit that extracts an area of a target portion that satisfies the condition based on the dye amount of the molecule target dye.

The invention discloses a method for plasticizing a biological specimen. The method is characterized by comprising the following steps of: a, immobilizing the biological specimen, namely completely immobilizing the biological specimen by using formalin; b, dehydrating and degreasing, namely manufacturing the immobilized biological specimen, and then replacing by using acetone until the water content of the specimen is less than 1 percent; c, performing vacuum impregnation, namely impregnating the completely replaced biological specimen in a biological vacuum impregnation plasticizer to finishthe replacement in a vacuum negative-pressure freezer; d, baking by a baking oven, namely putting the completely replaced biological specimen into the baking oven for high-temperature baking, and completely removing the excessive biological vacuum impregnation plasticizer in the biological specimen; and e, hardening, namely putting the biological specimen baked by the baking oven into an airtightcontainer for hardening. The method effectively shortens the manufacturing time of the specimen, and is easy to operate; and the overall shape of the specimen is not changed, and the specimen has strong sense of reality and certain flexibility, so that the specimen observation at the later stage is facilitated.

It is an object of the present invention to improve the stability of a gas field ionization ion source.

A GFIS according to the present invention is characterized in that the aperture diameter of the extraction electrode can be set to any of at least two different values or the distance from the apex of the emitter to the extraction electrode can be set to any of at least two different values. In addition, solid nitrogen is used for cooling. According to the present invention, it is possible to not only let divergently emitted ions go through the aperture of the extraction electrode but also, in behalf of differential pumping, reduce the diameter of the aperture. In addition, it is possible to reduce the physical vibration of the cooling means. Consequently, it is possible to provide a highly stable GFIS and a scanning charged particle microscope equipped with such a GFIS.

To prevent the displacement from an optical axis of a charged particle beam from being made independent of the direction (parallel to or perpendicular to the optical axis) of a magnetic field applied to a specimen, a system including an electron microscope and using a charged particle beam optical system is provided with a source of a charged particle beam, a condenser optical system, a specimen to be observed, a system for applying a magnetic field to the specimen, an imaging optical system and an image observation/recording apparatus, is provided with first and second charged particle beam deflection systems in order along a direction in which the charged particle beam travels between the condenser optical system and the specimen, is provided with third and fourth charged particle beam deflection systems in order between the specimen and the imaging lens system, and the quantity and the direction of the deflection of the charged particle beam by each deflection system and the intensity and the bearing of a magnetic field applied to the specimen are related according to predetermined relation.

An electron beam observation device includes a mechanism which disposes a specimen at an upstream side in an electron beam traveling direction outside an objective lens, from which an image is transferred under a magnification of ⅕ to 1/30, in addition to an inside of the objective lens in which a specimen is disposed at a time of ordinary observation.

It is an object of the present invention to provide a specimen observation method, an image processing device, and a charged-particle beam device which are preferable for selecting, based on an image acquired by an optical microscope, an image area that should be acquired in a charged-particle beam device the representative of which is an electron microscope. In the present invention, in order to accomplish the above-described object, there are provided a method and a device for determining the position for detection of charged particles by making the comparison between a stained optical microscope image and an elemental mapping image formed based on X-rays detected by irradiation with the charged-particle beam.

The invention relates to a manufacturing method of biological plasticized specimen capable of being assembled, and belongs to the technical field of biological plasticization. The specimen is manufactured via the steps of material selection, anticorrosion treatment, dissection, dehydration and degreasing, vacuum dipping, installation of a magnetic material, combination, solidification and conditioning. According to the manufacturing method of the invention, a real organism serves as the specimen, the general shape of the specimen is not changed, the specimen feels vivid, convenience is provided for post specimen observation, and use and operation are simple; the specimen can be dismounted and combined, the specimen can be manufactured part by part according to teaching materials or explained/learned layer by layer, convenience is provided for teaching and learning, reference is provided for making operation approaches and schemes for clinical doctors, and one specimen of the invention can realize functions of multiple other specimens; and the specimen is nontoxic, tasteless and easy to store, and can be placed in the air for a long time, and a healthy product is provided for users.

The invention relates to a biological specimen serving as a surgical instrument and a manufacturing method thereof. The biological specimen serving as the surgical instrument includes a human body specimen, the arteries and veins of the human body specimen are filled with silica gel pigments different in color, the other tissues of the human body specimen are filled with liquid silica gel for exchanging moisture and fat through extrusion, and skeletons of the human body specimen are provided with fracture apparatuses. A real creature is adopted as the specimen, the shape of the specimen is notchanged basically, the sense of reality is high, and more convenience is provided for specimen observation in the later period; moreover, the use and operation are simple, the positions of the apparatuses and the surrounding anatomical structures can be clearly displayed, and more convenience is provided for teachers during teaching and for students during study.

The invention relates to an animal muscle plasticizing specimen and a preparing method thereof, and belongs to the technical field of biological plasticizing. The preparing method of the animal muscleplasticizing specimen comprises the following steps of stripping skin of an animal specimen which is subjected to preservative treatment to obtain a specimen showing muscular tissue; bleaching the specimen showing the muscular tissue to obtain a stripped specimen; placing the stripped specimen into an acetone solution to be soaked for 80-90 d; afterwards, putting the specimen into a silica gel mixture for standing for 60-70 d, adjusting the pressure during standing, gradually adjusting the pressure to 1.5 kPa from 0.1 kPa, and replacing the acetone solution by squeezing the silica gel mixturein the specimen; putting the impregnated specimen in a curing box after shaping and repairing the impregnated specimen, and injecting a first silica gel curing agent into the impregnated specimen, sothat the silica gel mixture is cured, and then utilizing steam of a second silica gel curing agent for steam curing to make the impregnated specimen cured as a whole to obtain the animal muscle plasticizing specimen. The animal muscle plasticizing specimen is entire in shape and structure without any change, has strong reality sense and facilitates specimen observation in the later period.

When specimen (4) is placed on specimen stage (2), controller (32)—via the stage driver (33) and drive mechanism (6)—moves the specimen stage (2) by a predetermined step pitch according to the magnification factor in the X-direction and the Y-direction, and the image pickup unit (7) acquires a microscopic observation image of the specimen (4) after each move. The microscopic observation image that is acquired and the position data of the specimen stage (2) when the image is acquired are stored in memory (321). When a plurality of microscopic observation images of the areas that are adjacent on the specimen (2) are obtained, the image integration processor (322) uses the position data to join the microscopic observation images. When a plurality of microscopic observation images that encompasses the entirety of the specimen (2) is all joined together to form a specimen observation image, the specimen observation image is displayed on a display unit 37. The user then specifies the desired measurement region based on the specimen observation image that is high in spatial resolution and covering a wide area. Because of this, there is no need to repeat the steps of image pickup and mass spectrometry even when performing a mass spectrometry over a wide area, allowing an efficient measurement.

The present invention discloses an acer palmatum thunb variety acer matsumurae green retention method, and belongs to the technical field of plant specimen production. According to the present invention, Mg<2+> in the leaf is replaced with Cu<2+> so as to achieve the long-term green retention of the green leaf, such that the plant specimen is retained for a long time, the morphological structure of the material is preserved at a maximum, the specimen observation clarity is increased, the problems that the long-term green retention of the green plant specimen cannot be achieved and the environmental pollution exists during the specimen preservation and storage are solved, and the good application prospects are provided in the plant research and plant viewing fields.

A vacuum condition controlling apparatus, the top of which is connected with an electron beam generating instrument. The apparatus is rotationally symmetric, comprises the following parts deployed outward from the central axis: the central channel, the first pumping channel, the gas supplying chamber and the at least one pumping chamber. A pressure limiting aperture is deployed near the outlet of the central channel, for keeping the pressure difference between the central channel and the outside environment, and allow the electron beam to go through the central channel; the first pumping channel is connected to the central channel to pump the central channel; the top of the gas supplying chamber is connected to the gas supplying channel to supply gas to the area between the specimen and the apparatus; the top of the second pumping channel is connected to the second pumping channel, to pump the area.

It is possible to readily observe pathology specimens without spending much time and suspected pathology specimens in detail. A specimen observation device comprises an image capturing unit acquiring a partial image representing at least a part of one of multiple pathology specimens mounted on an accommodating section and a whole image of the multiple pathology specimens mounted on the accommodating section; an input unit inputting identification information of the accommodating section; a display unit displaying an enlarged version of the partial image acquired by the image capturing unit); an image designating unit designating the partial image displayed on the display unit; and a storage unit storing the identification information input via the input unit and a position of the partial image designated via the image designating unit in relation to the whole image such that the position and the identification information are associated with the whole image.

The invention relates to a plasticized specimen for the positional relationship between a spinal cord and an intervertebral disc and a manufacturing method of the plasticized specimen. The manufacturing method of the plasticized specimen for the positional relationship between the spinal cord and the intervertebral disc comprises the following steps of carrying out antiseptic treatment on a selected human body specimen; preparing materials for manufacturing, completely reserving the intervertebral disc, cleaning the spinal cord and the spinal nerve at a vertebral body removal position, and clearly displaying the positional relationship between the spinal nerve and the intervertebral disc; bleaching the specimen; soaking the bleached specimen in an acetone solution for 50-70 days; placing the dehydrated and degreased specimen in silica gel in a vacuum pressure bin for standing for 40-50 days; taking out the impregnated specimen from silica gel, shaping and repairing; placing the specimen in a curing box and curing with a curing agent for 10-15 days. According to the preparation method of the specimen, a real organism is taken as the specimen, the shape and structure of the specimenare complete and unchanged, the reality is high, later specimen observation is facilitated, the use and operation are simple, teaching by teachers and learning by students are facilitated, the specimen is nontoxic and tasteless, and long-term storage is facilitated.

For a novice user to easily recognize a difference between imaging results caused by a difference between observation conditions, a computer has an operation screen display observation target setting buttons for changing an observation condition for a specimen including a combination of parameter setting values of a charged particle beam apparatus. The processing unit has the operation screen display a radar chart including a characteristic, indicated by three or more incompatible items, of an observation condition for each of the observation target setting buttons. The radar chart indicates at least items of high resolution, emphasis on surface structure and emphasis on material difference.

Skills of a novice user operating a charged particle beam apparatus can be improved. Provided are an image display device which displays operation items of an electron microscope on an operation screen, a storage device which stores information of assist buttons which display image state information acquired via a detector of the electron microscope such that the information of assist buttons is correspondent to image quality of the image thus acquired as well as to observation conditions composed of a combination of parameter setting values of the electron microscope, an operation program which analyzes the image quality of the image acquired via the detector, acquires the information of the assist buttons based on analytical results of the image quality of the image as well as current observation conditions, and makes the assist buttons be displayed on the predetermined part of the operation screen.

The invention relates to a biological plasticized specimen capable of being opened from back to display the visceral positional relationship, and a preparation method thereof, and belongs to the technical field of biological plasticization. The preparation method comprises the following steps: 1, breaking ribs of a preservative-treated human body specimen from the back to expose visceral organs from the back, separating the sacrum and the hip bone at the sacroiliac joint in a manner of complete separation at one side and ligament reservation at the other side, bleaching the human body specimen, soaking the dissected human body specimen in an acetone solution for 60-80 d, putting the dehydrated and degreased human body specimen in a mixed solution of silica gel and a silica gel catalyst ina vacuum pressure cabin, standing for 50-60 d, adjusting the pressure of the vacuum pressure bin from 0.1-0.3 kPa to 0.9-1.2 kPa during standing, shaping and repairing the impregnated human body specimen, putting the human body specimen in a curing box, and curing the human body specimen by using organic peroxide and aliphatic nitrogen compound steam to obtain the biological plasticized specimen.The biological plasticized specimen displays the shape and position relation of visceral organs from the back, so later specimen observation is facilitated.