The application discloses a
microscope-based biological experiment operation evaluation method and device and readable medium, wherein the method comprises the following steps: acquiring and
processing the light-focusing image of the
microscope in the biological experiment operation, performing
edge detection on the processed light-focusing image to obtain edge information and determine the state of the positioning circle in the light-focusing image; acquiring the component state detected by the light-focusing operation component detection sensor, determining the light-focusing step operation result according to the state of the positioning circle and the component state; acquiring the
specimen observation image of the
microscope at different times in the biological experiment operation and
processing the
specimen observation image, obtaining the black-and-white pixel point proportion and
Gaussian blur difference value according to the processed
specimen observation image, and calculating the
clarity factor; acquiring the trigger state detected by the focusing operation component trigger sensor, and determining the observation step operation result according to the change process of the
clarity factor corresponding to the specimen observation image at different times and the trigger state. The method can reduce the computer computing power requirement and reduce the deployment cost.