Method for plasticizing biological specimen

A biological specimen, biological technology, applied in the fields of botany equipment and methods, preservation of plants, preservation of human or animal bodies, etc. Production time, strong sense of realism, simple operation effect

Active Publication Date: 2011-07-20
山东聚众数字医学科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. In the dehydration step, use low temperature to gradually increase the concentration of acetone replacement or pure acetone replacement. Using low temperature to gradually increase the concentration of acetone replacement needs to pre-cool the acetone for 1 week, and the specimen needs to be pre-cooled for 24 to 48 hours. This is not only wasteful, but also requires conditions. Very high, a large investment can only be carried out in a low-temperature freezer; and the dehydration cycle is long, up to several months, requiring a large amount of investment, so it is not suitable for operation
When using pure acetone to replace the specimen during dehydration, because the dehydration range of the specimen is very large, it is easily deformed, the shrinkage is serious, and the general shape of the specimen is seriously changed, so scientific research is not used; and pure acetone dehydration requires a large amount of investment, which is easy

Method used

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  • Method for plasticizing biological specimen
  • Method for plasticizing biological specimen

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: a kind of plasticizing method of biological specimen, it comprises the steps:

[0046] a. Biological specimen fixation: Firstly, perfuse the biological specimen with formalin with a volume concentration of 5%. After 10 days, the formalin penetrates into various parts of the biological specimen, and then completely immerse the entire biological specimen in the formalin solution , after 6 months, it is completely fixed, and the specimen can be anatomically manipulated;

[0047]b. Dehydration and degreasing: After the fixed biological specimens have been prepared, they are washed under running water for 48 hours and then quickly placed in an acetone solution with a volume concentration of 50% and a temperature of 18-25 degrees Celsius. The acetone is replaced, and the acetone is replaced until the water content of the specimen is less than 1%. The process of acetone replacement is carried out step by step, specifically: the volume ratio of the acetone soluti...

Embodiment 2

[0054] Embodiment 2: a kind of plasticizing method of biological specimen, it comprises the steps:

[0055] a. Biological specimen fixation: Firstly, perfuse the biological specimen with formalin with a volume concentration of 15%. After 7 days, the formalin penetrates into various parts of the biological specimen, and then completely immerse the entire biological specimen in the formalin solution , after 4 months, it is completely fixed, and the specimen can be anatomically manipulated;

[0056] b. Dehydration and degreasing: After the fixed biological specimens are prepared, they are washed under running water for 24 hours and then quickly placed in an acetone solution with a volume concentration of 50% and a temperature of 18-25 degrees Celsius, so that the water and fat in the tissue are separated from the tissue. The acetone is replaced, and the acetone is replaced until the water content of the specimen is less than 1%. The process of acetone replacement is carried out ...

Embodiment 3

[0063] Embodiment 3: a kind of plasticizing method of biological specimen, it comprises the steps:

[0064] a. Biological specimen fixation: Firstly, perfuse the biological specimen with formalin with a volume concentration of 10%. After 8 days, the formalin penetrates into various parts of the biological specimen, and then completely immerse the entire biological specimen in the formalin solution , after 5 months, it is completely fixed, and the specimen can be anatomically manipulated;

[0065] b. Dehydration and degreasing: After the fixed biological specimens have been prepared, they are rinsed under running water for 36 hours and then quickly placed in an acetone solution with a volume concentration of 50% and a temperature of 18-25 degrees Celsius, so that the water and fat in the tissue and The acetone is replaced, and the acetone is replaced until the water content of the specimen is less than 1%. The process of acetone replacement is carried out step by step, specifi...

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Abstract

The invention discloses a method for plasticizing a biological specimen. The method is characterized by comprising the following steps of: a, immobilizing the biological specimen, namely completely immobilizing the biological specimen by using formalin; b, dehydrating and degreasing, namely manufacturing the immobilized biological specimen, and then replacing by using acetone until the water content of the specimen is less than 1 percent; c, performing vacuum impregnation, namely impregnating the completely replaced biological specimen in a biological vacuum impregnation plasticizer to finishthe replacement in a vacuum negative-pressure freezer; d, baking by a baking oven, namely putting the completely replaced biological specimen into the baking oven for high-temperature baking, and completely removing the excessive biological vacuum impregnation plasticizer in the biological specimen; and e, hardening, namely putting the biological specimen baked by the baking oven into an airtightcontainer for hardening. The method effectively shortens the manufacturing time of the specimen, and is easy to operate; and the overall shape of the specimen is not changed, and the specimen has strong sense of reality and certain flexibility, so that the specimen observation at the later stage is facilitated.

Description

Technical field: [0001] The invention relates to a method for plasticizing biological specimens, belonging to the technical field of biological plasticization. Background technique: [0002] At present, there are following disadvantages in the existing methods of plasticizing biological specimens: [0003] 1. In the dehydration step, use low temperature to gradually increase the concentration of acetone replacement or pure acetone replacement. Using low temperature to gradually increase the concentration of acetone replacement needs to pre-cool the acetone for 1 week, and the specimen needs to be pre-cooled for 24 to 48 hours. This is not only wasteful, but also requires conditions. It is very high, and the investment is very large. It can only be carried out in a low-temperature freezer; and the cycle time of dehydration is long, up to several months, and it needs to invest a lot of money, so it is not suitable for operation. When using pure acetone to replace the specimen...

Claims

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Application Information

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IPC IPC(8): A01N1/00A01N3/00G09B23/00C08L83/06C08L83/04C08K5/5415C08K5/544
Inventor 黄飞张春雷张路萍周帅
Owner 山东聚众数字医学科技开发有限公司
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