Method for plasticizing biological specimen

Abstract

The invention discloses a method for plasticizing a biological specimen. The method is characterized by comprising the following steps of: a, immobilizing the biological specimen, namely completely immobilizing the biological specimen by using formalin; b, dehydrating and degreasing, namely manufacturing the immobilized biological specimen, and then replacing by using acetone until the water content of the specimen is less than 1 percent; c, performing vacuum impregnation, namely impregnating the completely replaced biological specimen in a biological vacuum impregnation plasticizer to finishthe replacement in a vacuum negative-pressure freezer; d, baking by a baking oven, namely putting the completely replaced biological specimen into the baking oven for high-temperature baking, and completely removing the excessive biological vacuum impregnation plasticizer in the biological specimen; and e, hardening, namely putting the biological specimen baked by the baking oven into an airtightcontainer for hardening. The method effectively shortens the manufacturing time of the specimen, and is easy to operate; and the overall shape of the specimen is not changed, and the specimen has strong sense of reality and certain flexibility, so that the specimen observation at the later stage is facilitated.

Description

Technical field: [0001] The invention relates to a method for plasticizing biological specimens, belonging to the technical field of biological plasticization. Background technique: [0002] At present, there are following disadvantages in the existing methods of plasticizing biological specimens: [0003] 1. In the dehydration step, use low temperature to gradually increase the concentration of acetone replacement or pure acetone replacement. Using low temperature to gradually increase the concentration of acetone replacement needs to pre-cool the acetone for 1 week, and the specimen needs to be pre-cooled for 24 to 48 hours. This is not only wasteful, but also requires conditions. It is very high, and the investment is very large. It can only be carried out in a low-temperature freezer; and the cycle time of dehydration is long, up to several months, and it needs to invest a lot of money, so it is not suitable for operation. When using pure acetone to replace the specimen...

AI Technical Summary

Problems solved by technology

[0003] 1. In the dehydration step, use low temperature to gradually increase the concentration of acetone replacement or pure acetone replacement. Using low temperature to gradually increase the concentration of acetone replacement needs to pre-cool the acetone for 1 week, and the specimen needs to be pre-cooled for 24 to 48 hours. This is not only wasteful, but also requires conditions. Very high, a large investment can only be carried out in a low-temperature freezer; and the dehydration cycle is long, up to several months, requiring a large amount of investment, so it is not suitable for operation

When using pure acetone to replace the specimen during dehydration, because the dehydration range of the specimen is very large, it is easily deformed, the shrinkage is serious, and the general shape of the specimen is seriously changed, so scientific research is not used; and pure acetone dehydration requires a large amount of investment, which is easy Constrained by real financial problems

[0004] 2. In the vacuum impregnation step, the mixture of plasticizer and catalyst cannot be replaced with the acetone in the biological specimen in a short time, especially the replacement of the deep structure, which prolongs the time for specimen preparation; and the pressure injection molding device is used to make the operation cumbersome steps

[0005] 3. There is no oven baking step, and the plasticizer immersed in the body cannot be baked to the maximum extent, so that the hardness of the biological specimen is too high when it is hardened, and the biological specimen cannot maintain a certain degree of flexibility; it does not have a sense of reality, that is, the surface of the specimen There will be a clear gelatinous substance

[0006] 4. There are restrictions on hardening conditions in the hardening step, and the effect of hardened specimens is not ideal, and the specimens lack realism and flexibility

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