Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of eIFiso4G2 protein for regulating tolerance of plants to ABA

A tolerance, plant technology, applied in the fields of application, plant peptides, plant products, etc., can solve the problems of agricultural production loss, consumption of nutrients and storage substances, seed edible quality, storage quality decline, etc.

Inactive Publication Date: 2019-07-12
TSINGHUA UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fetal germination consumes part of its nutrients and storage substances, resulting in a decline in the edible quality and storage quality of the seeds, causing greater losses to agricultural production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of eIFiso4G2 protein for regulating tolerance of plants to ABA
  • Application of eIFiso4G2 protein for regulating tolerance of plants to ABA
  • Application of eIFiso4G2 protein for regulating tolerance of plants to ABA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, the acquisition and identification of transgenic plants

[0065] 1. Obtaining of Mutant ei4g2-1

[0066] Seeds of Arabidopsis thaliana with the number SALK_076633 (the background is Arabidopsis ecotype Columbia, a T-DNA insertion mutant) were commercially purchased from the Arabidopsis Biological Research Center, and grown into plants. Genomic DNA was extracted from the plants at the seedling stage, and PCR identification was carried out with the primer pair consisting of ei4g2-1 LP and ei4g2-1 RP and the primer pair consisting of LBb1.3 and ei4g2-1 RP, respectively. If the amplified product is not detected by the primer pair composed of ei4g2-1 LP and ei4g2-1 RP, and the amplified product can be detected by the primer pair composed of LBb1.3 and ei4g2-1 RP, it proves that the plant is two Chromosomally identical homozygous mutants. Homozygous mutants were sequenced for verification.

[0067] ei4g2-1 LP: 5'-ATGCAACAACAAGGTGAACC-3';

[0068] ei4g2-1 RP:...

Embodiment 2

[0092] Embodiment 2, the tolerance of plant to ABA

[0093] 1. Seed Germination Experiment

[0094] ABA plays an important regulatory role in promoting seed dormancy and inhibiting seed germination. Genetic studies have shown that chromatin remodeling, histone methylation and deacetylation during seed germination are all regulated by ABA. With the increase of exogenous ABA concentration, the germination rate of wild-type Arabidopsis seeds will gradually decrease. Statistical seed germination experiment is one of the important methods to study ABA signal transduction.

[0095] The tested seeds were: seeds of Arabidopsis thaliana ecotype Colombia, and seeds of mutant ei4g2-1.

[0096] The first group: sow the tested seeds on the MS medium plate, stratify at 4°C for 3 days, then transfer to the light incubator for cultivation, and culture in the light incubator for 36 hours, 48 ​​hours or 72 hours, then count Seed germination rate.

[0097] The second group: Sow the tested s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of eIFiso4G2 protein for regulating tolerance of plants to ABA. The application of the eIFiso4G2 protein for regulating tolerance of the plants to ABA, the eIFiso4G2 protein is used for protecting substances which inhibit expression of specific genes in the plants, regulating the tolerance of the plants to abscisic acid, selectively breeding plant varieties of which the tolerance to the abscisic acid is reduced, regulating vivipary resistance of the plants and selectively breeding plant varieties with increasing vivipary resistance, and the specific genes are genes which encode the eIFiso4G2 protein. According to the application of the eIFiso4G2 protein for regulating tolerance of the plants to ABA, the eIFiso4G2 protein is an important negative regulator in conduction of ABA signals, and by knocking eIFiso4G2 genes out, the tolerance of the plants to the ABA can be obviously reduced. The application of the eIFiso4G2 protein for regulating toleranceof the plants to ABA accords with sustainable agricultural development requirements and has high practical value and market prospects on cultivation of new efficient ABA-tolerating adversity stress varieties.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of eIFiso4G2 protein in regulating plant tolerance to ABA. Background technique [0002] Abscisic acid (ABA) is one of the important hormones in plants. It plays an important role in all stages of plant growth and development, including seed dormancy, germination, seedling growth, stomatal movement, and transition from vegetative growth to reproductive growth. Wait for the process. At the same time, ABA plays an important role in the response of plants to various external adversity stresses, such as abiotic stresses such as drought, low temperature, high temperature, salt, mechanical damage, and biotic stresses such as pests and diseases. Therefore, the elucidation of the molecular mechanism of the plant ABA signal transduction pathway is of great significance for regulating plant growth, improving crop quality, improving genetic characteristics, and prom...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K19/00C12N15/82A01H5/00
CPCC07K14/415C07K2319/00C12N15/8293
Inventor 张大鹏马宇毕超
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com