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A method for improving plant tolerance to drought by down-regulating eif4g gene and eifiso4g1 gene

A plant and gene technology, applied in the fields of botanical equipment and methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as the difficulty of stress tolerance traits

Inactive Publication Date: 2020-10-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is relatively difficult to cultivate and improve stress tolerance traits by traditional breeding techniques, and it is difficult to obtain excellent drought-resistant varieties

Method used

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  • A method for improving plant tolerance to drought by down-regulating eif4g gene and eifiso4g1 gene
  • A method for improving plant tolerance to drought by down-regulating eif4g gene and eifiso4g1 gene
  • A method for improving plant tolerance to drought by down-regulating eif4g gene and eifiso4g1 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, the acquisition and identification of mutants

[0063] 1. Obtaining of mutant e4g-2

[0064] Seeds of Arabidopsis thaliana with the number SALK_136398 (the background is Arabidopsis ecotype Columbia, a T-DNA insertion mutant) were commercially purchased from the Arabidopsis Biological Research Center, and grown into plants. The genomic DNA of the seedling stage plants was extracted, and PCR identification was carried out with the primer pair composed of e4g-2 LP and e4g-2 RP and the primer pair composed of LBb1.3 and e4g-2 RP, respectively. If the amplified product is not detected with the primer pair composed of e4g-2 LP and e4g-2 RP, and the amplified product can be detected with the primer pair composed of LBb1.3 and e4g-2RP, it proves that the plant has two chromosomes Consistent homozygous mutants. Homozygous mutants were sequenced for verification.

[0065] e4g-2 LP: 5'-AACCTTCTTCGTTCTCTTCGC-3';

[0066] e4g-2 RP: 5'-CTGACAACGCCTTAAACGAAG-3';

...

Embodiment 2

[0106] Embodiment 2, the resistance of plant to drought stress

[0107] 1. ABA promotes stomatal closure and inhibits stomatal opening experiment

[0108] ABA is an important signaling molecule for plants to resist external adversity stress. Under drought conditions, ABA can promote stomatal closure to reduce water loss by regulating ion channels in stomatal guard cells. Calcium ions, protein kinases, and phosphatases are all involved in ABA-mediated guard cell signal transduction and the signal regulation of ABA-related stress resistance genes. Therefore, ABA-induced stomatal movement experiments were used to detect whether eIF4G protein and eIFiso4G1 protein play a role in the process of ABA regulating plant drought stress response.

[0109] Strip buffer: MES-KOH (pH 6.15) containing 50 mM KCl and 10 mM.

[0110] The light intensity for culturing under a cold light source was 200 μM Photons m -2 the s -1 .

[0111] 1. ABA inhibits stomatal opening experiment

[0112] ...

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Abstract

The invention discloses a method for improving the tolerance of plants to drought by down-regulating an eIF4G gene and an eIFiso4G1 gene. The invention provides application of an expression substanceused for inhibiting a specific gene A in the plants and an expression substance used for inhibiting a specific gene B in the plants, which comprises regulation of the drought resistance of the plantsand breeding of plant varieties with improved drought resistance. The specific gene A is a gene encoding eIF4G protein. The specific gene B is a gene encoding eIFiso4G1 protein. The inventor finds that the knockout of the eIF4G gene and eIFiso4G1 gene can make the tolerance of the plants to the drought significantly enhanced. The method for improving the tolerance of the plants to the drought by down-regulating the eIF4G gene and the eIFiso4G1 gene meets the requirements of sustainable agricultural development, and has important practical value and market prospects for studying the molecular mechanism of drought tolerance of the plants, improving genetic characteristics, improving the resistance of crops to natural climatic disasters and adverse soil environment, cultivating novel high-efficiency drought-resistant varieties and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving plant tolerance to drought by down-regulating eIF4G gene and eIFiso4G1 gene. Background technique [0002] Terrestrial plants may be affected by a variety of external adversity stresses throughout their growth and development stages. Among them, abiotic stresses such as drought, salinity, low temperature, and high temperature, and biotic stresses such as pests and diseases significantly affect plant growth and crop yield. The research on plant stress resistance has always been a hot spot in the field of botany. It is relatively difficult to cultivate and improve stress-tolerant traits by traditional breeding techniques, and it is difficult to obtain excellent drought-resistant varieties. With the development of molecular biology technology and the in-depth study of the molecular mechanism of plant stress resistance, the genetic engineering of plant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/20
CPCC07K14/415C12N15/8273
Inventor 张大鹏毕超马宇
Owner TSINGHUA UNIV
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