32results about How to "Meet the needs of sustainable agricultural development" patented technology

The invention discloses a method for improving tolerance of plants for NaCl by reducing the content of PAB2 and PAB4. The method specifically provides an application of protein 1 and protein 2 serving as targets to any one of the following conditionsthat a1, salt tolerance of the plants is improved; a2, plant varieties with improved salt resistance are bred. Protein 1 is PAB2, and protein 2 is PAB4. It is found through the research that tolerance of PAB2 and PAB4 gene double knockout mutants obtained in the arabidopsis thaliana bioresearch center to NaCl is enhanced; thus, the tolerance of the plants to NaCl can be adjusted through PAB2 and PAB4; in addition, PAB2 and PAB4 low-expression and salt-resistant transgenic crops can be obtained through the gene engineering means (PNAi technology). The method meets the sustainable agriculture development demand, and high practical value and market prospects are achieved on the aspects of researching the molecular mechanism for plants to tolerant NaCl, improving hereditary characters, cultivating efficient salt-resistant new varieties and the like.

The invention discloses application of a CPN20 protein and a coding gene thereof in regulating the ABA (abscisic acid) tolerance of a plant. The application provided by the invention is particularly application of a protein composed of an amino acid sequence as shown in Sequence 3 in a sequence table in a1) or a2) below: a1) regulating the ABA tolerance of a plant; and a2) breeding ABA tolerance improved plant species. The invention can be used for using phytohormone ABA as a selective weedicide, the selectivity depends on the ABA sensitivity of the plant, and the CPN20 gene is used to regulate the ABA sensitivity or tolerance of the plant, thus obtaining CPN20 gene highly-expressed anti-weedicide transgenic crops and further realizing selective weed control. The invention meets the sustainable agricultural development requirements, and has important practical values and market prospects in the aspects of improving hereditary characters, developing green environment-friendly nuisance-free weed control methods and approaches and the like.

The invention discloses application of NF-YC9 protein in regulating and controlling ABA tolerance of plants. The application specifically refers to application of the NF-YC9 protein or coding gene thereof in regulating and controlling ABA tolerance of the plants. An amino acid sequence of the NF-YC9 protein can be shown as a sequence 3. Experiments prove that tolerance of the plants to ABA is lowered after overexpression of the NF-YC9 protein. Therefore, the NF-YC9 can be used as a growth studying model for studying important genes in regulating and controlling crop growth and development and environment stress and is conducive to deeply clarifying needed molecular mechanism of positive-negative adjusting factors in regulating and controlling response level of the plants to ABA signals, plant growth and stress resistance to meet needs of agricultural production, so that theoretical support is provided for actual agricultural production. The NF-YC9 protein meets development needs of sustainable agriculture and has important practical value and market prospect in the aspects of studying ABA-tolerant molecular mechanism of the plants and crop improvement hereditary characteristics.

The invention discloses a gene for regulating plant seed germination and seedling growth, its encoded protein and application. The inventors of the present invention identified and obtained the NtCIPK23 gene from the common tobacco (Nicotiana tabacum) cultivar Zhongyan 100, the nucleotide sequence of the NtCIPK23 gene is shown in SEQ ID NO.1, and proved by transgenic technology that overexpressing NtCIPK23 can Accelerate the germination rate of tobacco seeds and promote the growth of seedlings, while knocking out the gene will delay the germination rate of seedlings and inhibit the growth of seedlings. By using the present invention, the germination rate and uniformity of plant seeds can be controlled by adjusting the expression level of CIPK23, and the transgenic crops with moderate expression of CIPK23 gene and uniform emergence rate can be obtained by means of genetic engineering. The invention meets the requirements of sustainable agricultural development, and has important practical value and market prospect for cultivating economic crops that grow early, grow fast, and emerge neatly.

The invention discloses a method for improving tolerance of plants to NaCl by lowering PAB4 and PAB8. The invention provides application of protein 1 and protein 2 used as targets in improving salt tolerance of plants or selecting and breeding plant varieties with improved salt tolerance; the protein 1 is PAB4 protein, and the protein 2 is PAB8 protein. Researches show that the tolerance of a PAB4 and PAB8 gene double knockout mutant obtained from the arabidopsis thaliana biological research center to NaCl is enhanced; accordingly, the tolerance of plants to NaCl can be adjusted through PAB4 and PAB8. In addition, PAB4 and PAB8 low-expression and salt-tolerance transgenic crops can be obtained through a genetic engineering means (RNAi technology). The method meets the requirements for sustainable agriculture development, and has the important practical value and market prospect in the aspects of researching molecular mechanisms of tolerance of plants to NaCl, improving hereditary characters, breeding efficient salt-tolerance new variety and the like.

The invention discloses the application of the eIFiso4G2 protein in regulating the tolerance of plants to ABA. The present invention claims to protect the application of substances used to inhibit the expression of specific genes in plants: regulating the tolerance of plants to abscisic acid; breeding plant varieties with reduced tolerance to abscisic acid; regulating the embryonic resistance of plants; breeding A plant variety with increased fetal germination resistance; the specific gene is the gene encoding eIFiso4G2 protein. The present invention finds that the eIFiso4G2 protein is an important negative regulator in ABA signal transduction, and knocking out the eIFiso4G2 gene can significantly reduce the tolerance of plants to ABA. The invention meets the requirement of sustainable agricultural development, and has important practical value and market prospect for cultivating new varieties with high efficiency and tolerance to ABA and adversity stress.

The invention discloses application of MYB99 protein and coding genes thereof to control of plant seed germination. Specifically, the protein (namely the MYB99 protein) formed by an amino acid sequence shown as the sequence 3 in a sequence table is applied to (a1) control of vivipary resistance of a plant or (a2) breeding of a plant variety with improved vivipary resistance. According to the application, the germination rate of plant seeds can be controlled by regulating the expression level of the MYB99, and high-expression vivipary-resistant transgenic crops containing MYB99 genes are obtained by means of gene engineering. The application meets the sustainable agricultural development demand and has important practical value and market prospect for improving eating quality, storage quality and the like of seeds.