32results about How to "Meet the needs of sustainable agricultural development" patented technology

The invention discloses a method for promoting rapid propagation of arbuscular mycorrhizal fungal spores. According to the method, an arbuscular mycorrhizal fungal inoculant can be obtained after synthetic hormone GR24 or spermidine is added exogenously and is cultivated under a room temperature condition for 3 months by adopting an arbuscular mycorrhizal fungi potted cultivation technology. The method has the advantages that the materials are easily obtained, the operation is simple, the cost is comparatively low, the culture cycle is short, the mycorrhizal infection rate is high, and the spore density of the obtained inoculant is comparatively high; a nin the method, a cultivation vessel is simple, and the risk of mixed fungi pollution is avoided, and therefore, the quality of the inoculant is comparatively high.

The invention discloses a compost tea for improving the soil hardness and a preparation method thereof, belonging to the technical field of soil improvement. The preparation method of the compost tea suitable for improving the hardness soil comprises three parts: material selection, preparation process and quality index selection. The applying method of the compost tea comprises the step of selecting parameters of applying condition, applying time and applying amount. The preparation method of the compost tea has the advantages of easily obtained materials, lower cost, simple preparation process and simple applying method. The preparation of the improved hardened soil of the compost tea has the advantages of high efficiency and short time, and the crumb structure of the hardened soil is remarkably improved 40-50 days after the application of the compost tea. Chemically synthesized substances are not needed in the materials, and the compost tea solution prepared is pollution-free to the environment, thereby conforming to the need of agricultural sustainable development.

The invention discloses a method for improving the tolerance of a plant to droughts through down-regulation of expression of an eIFiso4G1 gene and an eIFiso4G2 gene. Application of the method in inhibiting expression substances of a specific gene A and a specific gene B in the plant comprises regulation and control over drought tolerance of the plant and seed selection for plant varieties of the improved drought tolerance. The specific gene A is a gene for encoding eIFiso4G1 protein; the specific gene B is a gene for encoding eIFiso4G2 protein. The inventor discovers that through knockout of the eIFiso4G1 gene and the eIFiso4G2 gene, the tolerance of the plant to droughts can be significantly improved. The method accords with the demands of the sustainable agricultural development and hasimportant practical value and an important market prospect in the aspects of studying the drought-tolerance molecule mechanism of the plant, improving the hereditary characters of the plant, improvingthe stress resistance of the plant to natural climatic disasters and bad soil environments, cultivating new efficient drought-tolerance varieties and the like.

The invention discloses application of eIFiso4G1 protein to regulation and control of the drought resistance of plants. The application required for protecting substances used for inhibiting specificgene expression in the plants comprises the following steps of regulating and controlling the drought resistance of the plants; breeding plant varieties with improved drought resistance. The inventionalso provides a method for preparing transgenic plants. The method comprises the following steps that the specific gene expression in receiver plants is inhibited to obtain the transgenic plants; compared with the receiver plants, the drought resistance of the transgenic plants is improved. A specific gene is a gene encoding the eIFiso4G1 protein. The inventor finds that the drought tolerance ofthe plants can be obviously enhanced by knocking out an eIFiso4G1 gene. The sustainable agriculture development requirement is conformed; important practical values and market prospects are realized in aspects of studying the plant drought resistant molecular mechanism; improving the hereditary character, improving the stress resistance of the plants on natural climatic disasters and poor soil environment, culturing new efficient drought resistant varieties and the like.

The invention discloses a method for improving NaCl tolerance of plants by reducing PAB2 and PAB8. The method specifically provides any application of protein 1 and protein 2 which serve as targets as follows: a1) salt tolerance of the plants is improved; a2) plant varieties with salt tolerance improved are selected and bred, wherein the protein 1 is PAB2 protein, and the protein 2 is PAB8 protein. Research finds that NaCl tolerance of gene double knockout mutants of PAB2 and PAB8 obtained by an arabidopsis biological research center is enhanced. Accordingly, PAB2 and PAB8 can be used for adjusting the NaCl tolerance of the plants. Besides, genetically modified crops low in PAB2 and PAB8 expression and tolerant to salt can be obtained trough a genetic engineering means (RNAi technology). The method meets the requirement for sustainable agricultural development and has important practical value and market prospects in the aspects of research of plant NaCl tolerance molecular mechanism, improvement of genetic characteristics, culture of efficient salt-tolerant new varieties and the like.

The invention discloses a method for reducing a nitrogen fertilizer in a wheat-corn rotation system, belonging to the technical field of management of soil nitrogen nutrients. The method comprises the following steps: carrying out seed dressing on a Mn-Zn micronutrient fertilizer; adding a nitrification inhibitor and a coated controlled release nitrogen fertilizer when the nitrogen fertilizer is applied, and carrying out foliar topdressing of a boron fertilizer. By adopting the method, once fertilization of the nitrogen fertilizer in a wheat-corn planting system is achieved, the labor force is saved, materials are easily available, the operation process is simple, the fertilization method is simple and convenient, the cost is low, popularization is facilitated, and the application prospect is good. The application rate of the nitrogen fertilizer is obviously reduced, compared with the traditional fertilization in wheat and corn planting, the method has the advantage that the dosage of the nitrogen fertilizer is reduced by over 20%, loss of the nitrogen nutrients is obviously reduced, the pollution to water and an atmospheric environment is relieved, the environment risk is reduced, and the needs of agricultural sustainable development are satisfied.

The invention discloses application of MYB37 protein and coding genes thereof to control of plant seed germination. Specifically, the protein (namely the MYB37 protein) formed by an amino acid sequence shown as the sequence 3 in a sequence table is applied to (a1) improvement in vivipary resistance of a plant or (a2) breeding of a plant variety with improved vivipary resistance. According to the application, the germination rate of plant seeds can be controlled by regulating the expression level of the MYB37, and high-expression vivipary-resistant transgenic crops containing MYB37 genes are obtained by means of gene engineering. The application meets the sustainable agricultural development demand and has important practical value and market prospect for improving eating quality, storage quality and the like of seeds.

The invention discloses application of eIF4G protein to regulation and control of the tolerance of plants on ABA (abscisic acid). The application required for protecting substances used for inhibitingspecific gene expression in the plants comprises the following steps of regulating and controlling the tolerance of the plants on the ABA; breeding plant varieties with reduced tolerance on the ABA;regulating and controlling vivipary resistance of the plants; breeding plant varieties with increased vivipary resistance, wherein a specific gene is a gene encoding the eIF4G protein. The conditionsthat the eIF4G protein is an important negative regulator in ABA signal conduction, and the tolerance of the plants on the ABA can be obviously reduced by knocking out the eIF4G gene are found. The sustainable agriculture development requirement is conformed; important practical values and market prospects are realized in aspects of culturing new efficient ABA and adversity stress resistant varieties and the like.

The invention discloses a method for improving tolerance of plants for NaCl by reducing the content of PAB2 and PAB4. The method specifically provides an application of protein 1 and protein 2 serving as targets to any one of the following conditionsthat a1, salt tolerance of the plants is improved; a2, plant varieties with improved salt resistance are bred. Protein 1 is PAB2, and protein 2 is PAB4. It is found through the research that tolerance of PAB2 and PAB4 gene double knockout mutants obtained in the arabidopsis thaliana bioresearch center to NaCl is enhanced; thus, the tolerance of the plants to NaCl can be adjusted through PAB2 and PAB4; in addition, PAB2 and PAB4 low-expression and salt-resistant transgenic crops can be obtained through the gene engineering means (PNAi technology). The method meets the sustainable agriculture development demand, and high practical value and market prospects are achieved on the aspects of researching the molecular mechanism for plants to tolerant NaCl, improving hereditary characters, cultivating efficient salt-resistant new varieties and the like.

The invention discloses the application of the eIFiso4G1 protein in regulating the tolerance of plants to ABA. The present invention claims to protect the application of substances used to inhibit the expression of specific genes in plants: regulating the tolerance of plants to abscisic acid; breeding plant varieties with reduced tolerance to abscisic acid; regulating the embryonic resistance of plants; breeding A plant variety with increased fetal germination resistance; the specific gene is the gene encoding eIFiso4G1 protein. The present invention finds that the eIFiso4G1 protein is an important negative regulator in ABA signal transduction, and knocking out the eIFiso4G1 gene can significantly reduce the tolerance of plants to ABA. The invention meets the requirement of sustainable agricultural development, and has important practical value and market prospect for cultivating new varieties with high efficiency and tolerance to ABA and adversity stress.

The invention discloses crop straw leach liquor for improving fertilizer efficiency of mycorrhizal fungi fertilizer and a preparation method of the crop straw leach liquor, belonging to the field of fertilization of biofertilizer. The preparation method disclosed by the invention comprises three parts, namely material selection, preparation process selection and quality index selection; the method for fertilizing the crop straw leach liquor comprises parameter selection of fertilizing condition, fertilizing time and fertilizing amount. The material selected by the method is easy to obtain, low in cost, simple in preparation process and simple and fast in fertilizing method, and the prepared crop straw leach liquor is high in efficiency of promoting crop seedling to grow, and short in time. The biomass liveweight of the crop seeding is improved by over 30% after the crop straw leach liquor is watered about 40 days. The material adopted by the method is not added chemically synthesized substance, and the prepared crop straw leach liquor has no pollution on the environment and accords with the requirements of sustainable development of agriculture.

The invention discloses application of a CPN20 protein and a coding gene thereof in regulating the ABA (abscisic acid) tolerance of a plant. The application provided by the invention is particularly application of a protein composed of an amino acid sequence as shown in Sequence 3 in a sequence table in a1) or a2) below: a1) regulating the ABA tolerance of a plant; and a2) breeding ABA tolerance improved plant species. The invention can be used for using phytohormone ABA as a selective weedicide, the selectivity depends on the ABA sensitivity of the plant, and the CPN20 gene is used to regulate the ABA sensitivity or tolerance of the plant, thus obtaining CPN20 gene highly-expressed anti-weedicide transgenic crops and further realizing selective weed control. The invention meets the sustainable agricultural development requirements, and has important practical values and market prospects in the aspects of improving hereditary characters, developing green environment-friendly nuisance-free weed control methods and approaches and the like.

The invention discloses an application of an MYB28 protein and an encoding gene thereof in regulation and control of tolerance of plants on ABA. The application provided by the invention is an application of the protein as shown in a sequence 3 in the following aspects: (a1) regulation and control of the tolerance of plants on ABA; (a2) breeding of a plant variety which is lowered or increased in the tolerance on ABA; (a3) regulation and control of vivipary resistance of plants; and (a4) breeding of a plant variety which is increased or lowered in the vivipary resistance. The MYB28 protein can be applied to obtaining of MYB28 low-expressed and herbicide-resistant transgenic crops employing plant hormone ABA as a selective herbicide through genetic engineering means, so as to achieve selective weeding; the germination rate of plant seeds can also be controlled by adjusting the expression level of the MYB28; MYB28 gene high-expressed and vivipary-resistant transgenic crops can be obtained through the genetic engineering means; the application conforms to the requirements of sustainable agriculture development, and has important practical value and market prospects in the aspects such as development of green, environment-friendly and pollution-free weeding methods and improvement of eating quality and storage quality of seeds.

The invention discloses application of NF-YC9 protein in regulating and controlling ABA tolerance of plants. The application specifically refers to application of the NF-YC9 protein or coding gene thereof in regulating and controlling ABA tolerance of the plants. An amino acid sequence of the NF-YC9 protein can be shown as a sequence 3. Experiments prove that tolerance of the plants to ABA is lowered after overexpression of the NF-YC9 protein. Therefore, the NF-YC9 can be used as a growth studying model for studying important genes in regulating and controlling crop growth and development and environment stress and is conducive to deeply clarifying needed molecular mechanism of positive-negative adjusting factors in regulating and controlling response level of the plants to ABA signals, plant growth and stress resistance to meet needs of agricultural production, so that theoretical support is provided for actual agricultural production. The NF-YC9 protein meets development needs of sustainable agriculture and has important practical value and market prospect in the aspects of studying ABA-tolerant molecular mechanism of the plants and crop improvement hereditary characteristics.

The invention discloses a method for improving the tolerance of plants to drought by down-regulating an eIF4G gene and an eIFiso4G1 gene. The invention provides application of an expression substanceused for inhibiting a specific gene A in the plants and an expression substance used for inhibiting a specific gene B in the plants, which comprises regulation of the drought resistance of the plantsand breeding of plant varieties with improved drought resistance. The specific gene A is a gene encoding eIF4G protein. The specific gene B is a gene encoding eIFiso4G1 protein. The inventor finds that the knockout of the eIF4G gene and eIFiso4G1 gene can make the tolerance of the plants to the drought significantly enhanced. The method for improving the tolerance of the plants to the drought by down-regulating the eIF4G gene and the eIFiso4G1 gene meets the requirements of sustainable agricultural development, and has important practical value and market prospects for studying the molecular mechanism of drought tolerance of the plants, improving genetic characteristics, improving the resistance of crops to natural climatic disasters and adverse soil environment, cultivating novel high-efficiency drought-resistant varieties and the like.

The invention discloses a gene for regulating plant seed germination and seedling growth, its encoded protein and application. The inventors of the present invention identified and obtained the NtCIPK23 gene from the common tobacco (Nicotiana tabacum) cultivar Zhongyan 100, the nucleotide sequence of the NtCIPK23 gene is shown in SEQ ID NO.1, and proved by transgenic technology that overexpressing NtCIPK23 can Accelerate the germination rate of tobacco seeds and promote the growth of seedlings, while knocking out the gene will delay the germination rate of seedlings and inhibit the growth of seedlings. By using the present invention, the germination rate and uniformity of plant seeds can be controlled by adjusting the expression level of CIPK23, and the transgenic crops with moderate expression of CIPK23 gene and uniform emergence rate can be obtained by means of genetic engineering. The invention meets the requirements of sustainable agricultural development, and has important practical value and market prospect for cultivating economic crops that grow early, grow fast, and emerge neatly.

The invention discloses a method for improving tolerance of plants to NaCl by lowering PAB4 and PAB8. The invention provides application of protein 1 and protein 2 used as targets in improving salt tolerance of plants or selecting and breeding plant varieties with improved salt tolerance; the protein 1 is PAB4 protein, and the protein 2 is PAB8 protein. Researches show that the tolerance of a PAB4 and PAB8 gene double knockout mutant obtained from the arabidopsis thaliana biological research center to NaCl is enhanced; accordingly, the tolerance of plants to NaCl can be adjusted through PAB4 and PAB8. In addition, PAB4 and PAB8 low-expression and salt-tolerance transgenic crops can be obtained through a genetic engineering means (RNAi technology). The method meets the requirements for sustainable agriculture development, and has the important practical value and market prospect in the aspects of researching molecular mechanisms of tolerance of plants to NaCl, improving hereditary characters, breeding efficient salt-tolerance new variety and the like.

The invention discloses the application of the eIFiso4G2 protein in regulating the tolerance of plants to ABA. The present invention claims to protect the application of substances used to inhibit the expression of specific genes in plants: regulating the tolerance of plants to abscisic acid; breeding plant varieties with reduced tolerance to abscisic acid; regulating the embryonic resistance of plants; breeding A plant variety with increased fetal germination resistance; the specific gene is the gene encoding eIFiso4G2 protein. The present invention finds that the eIFiso4G2 protein is an important negative regulator in ABA signal transduction, and knocking out the eIFiso4G2 gene can significantly reduce the tolerance of plants to ABA. The invention meets the requirement of sustainable agricultural development, and has important practical value and market prospect for cultivating new varieties with high efficiency and tolerance to ABA and adversity stress.

The invention discloses application of MYB99 protein and coding genes thereof to control of plant seed germination. Specifically, the protein (namely the MYB99 protein) formed by an amino acid sequence shown as the sequence 3 in a sequence table is applied to (a1) control of vivipary resistance of a plant or (a2) breeding of a plant variety with improved vivipary resistance. According to the application, the germination rate of plant seeds can be controlled by regulating the expression level of the MYB99, and high-expression vivipary-resistant transgenic crops containing MYB99 genes are obtained by means of gene engineering. The application meets the sustainable agricultural development demand and has important practical value and market prospect for improving eating quality, storage quality and the like of seeds.

The invention discloses a method for improving the tolerance of a plant to droughts through down-regulation of expression of an eIFiso4G1 gene and an eIFiso4G2 gene. Application of the method in inhibiting expression substances of a specific gene A and a specific gene B in the plant comprises regulation and control over drought tolerance of the plant and seed selection for plant varieties of the improved drought tolerance. The specific gene A is a gene for encoding eIFiso4G1 protein; the specific gene B is a gene for encoding eIFiso4G2 protein. The inventor discovers that through knockout of the eIFiso4G1 gene and the eIFiso4G2 gene, the tolerance of the plant to droughts can be significantly improved. The method accords with the demands of the sustainable agricultural development and hasimportant practical value and an important market prospect in the aspects of studying the drought-tolerance molecule mechanism of the plant, improving the hereditary characters of the plant, improvingthe stress resistance of the plant to natural climatic disasters and bad soil environments, cultivating new efficient drought-tolerance varieties and the like.