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A method for increasing plant tolerance to NACL by downregulating Pab4 and Pab8

A plant and salt-tolerant technology, applied in botany equipment and methods, chemical equipment and methods, biochemical equipment and methods, etc., can solve problems such as plant abortion, and achieve the effect of improving genetic characteristics

Active Publication Date: 2019-02-26
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Arabidopsis PABP5 gene is anther-specifically expressed, and its deletion may cause plant abortion

Method used

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  • A method for increasing plant tolerance to NACL by downregulating Pab4 and Pab8
  • A method for increasing plant tolerance to NACL by downregulating Pab4 and Pab8
  • A method for increasing plant tolerance to NACL by downregulating Pab4 and Pab8

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1. Identification and expression analysis of pab4-1 mutant, pab8-1 mutant and pab4-1 pab8-1 double mutant

[0058] 1. Identification of pab4-1 mutant, pab8-1 mutant and pab4-1 pab8-1 double mutant

[0059] according to figure 1 The primer combination in the pab4-1 and pab8-1 double mutation homozygous plants were identified by PCR technology. In addition, homozygous plants of pab4-1 mutant and pab8-1 mutant were identified by the same method.

[0060] The primer sequences used are as follows:

[0061] pab4-1 LP: 5'-GAGAAGGCAATGCAGAAGTTG-3'(Left primer, LP)

[0062] pab4-1 RP: 5'-ACTACCTGCAGCTTAGCCCTC-3'(Right primer, RP)

[0063] pab8-1 LP: 5'-ATCAACAACAGCTTGTACCGG-3'(Left primer, LP)

[0064] pab8-1 RP: 5'-CAGTCTGTTTTCGGAAGCAAG-3'(Right primer, RP)

[0065] LBb1: 5'-GCGTGGACCGCTTGCTGCAACT-3'(Left border primer, LB)

[0066] 2. Analysis of corresponding gene expression in pab4-1 mutant, pab8-1 mutant and pab4-1 pab8-1 double mutation

[0067] Extract tot...

Embodiment 2

[0089] Example 2, analysis test of pab4-1 pab8-1 double mutant tolerance to NaCl

[0090] NaCl can inhibit the growth of plant seedlings. With the increase of exogenous NaCl concentration, the inhibition of Arabidopsis wild type (Col-0 ecotype) was enhanced, and the growth of its main root and leaves were affected to varying degrees. The experiment was repeated three times.

[0091]Arabidopsis wild type (Col-0 ecotype), pab4-1 pab8-1 double mutant plants were used as experimental materials. The seeds of each experimental material were sown on MS plates without NaCl, stratified at a low temperature for 3 days at 4°C, placed in a light incubator for 72 hours, and then the newly germinated seedlings of each experimental material were moved to a medium containing different concentrations of NaCl. On MS medium (0mM, 50mM, 100mM, 150mM and 200mM), observe the growth of Arabidopsis seedlings after vertical growth for 9 days.

[0092] The result is as Figure 5 As shown, on the med...

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Abstract

The invention discloses a method for improving tolerance of plants to NaCl by lowering PAB4 and PAB8. The invention provides application of protein 1 and protein 2 used as targets in improving salt tolerance of plants or selecting and breeding plant varieties with improved salt tolerance; the protein 1 is PAB4 protein, and the protein 2 is PAB8 protein. Researches show that the tolerance of a PAB4 and PAB8 gene double knockout mutant obtained from the arabidopsis thaliana biological research center to NaCl is enhanced; accordingly, the tolerance of plants to NaCl can be adjusted through PAB4 and PAB8. In addition, PAB4 and PAB8 low-expression and salt-tolerance transgenic crops can be obtained through a genetic engineering means (RNAi technology). The method meets the requirements for sustainable agriculture development, and has the important practical value and market prospect in the aspects of researching molecular mechanisms of tolerance of plants to NaCl, improving hereditary characters, breeding efficient salt-tolerance new variety and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a method for improving plant tolerance to NaCl by down-regulating PAB4 and PAB8. Background technique [0002] Salt stress is one of the main factors affecting crop yield. Salt stress mainly causes damage to plants through NaCl as a stress. The high concentration of sodium ions in the soil will directly affect the protein synthesis, photosynthesis and energy metabolism of plants, etc., which will lead to reduced plant germination rate, growth retardation, reduced protein synthesis and slowed metabolism, etc., which will seriously affect the yield of crops. So far, the total area of ​​saline land in the world is about 954.38 million hm 2 , and my country accounted for 99.13 million hm 2 , and the area of ​​global and my country's salinization is increasing every year. [0003] Under the severe situation of increasing saline land in my country, it is of great practical significance to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29C07K14/415A01H5/00A01H6/20
CPCC07K14/415C12N15/8273
Inventor 卢艳芬姚允聪张杰郝素晓陈琦于泳涛
Owner BEIJING UNIV OF AGRI
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