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Application of myb37 protein and its coding gene in regulating plant seed germination

An encoding gene and transgenic plant technology, applied to the application field of MYB37 protein and its encoding gene in regulating plant seed germination, can solve the problem of seed eating quality, storage quality decline, consumption of nutrients and storage substances, deterioration of seed quality, seed use Value and storability, etc., to improve the edible quality and storage quality of seeds, and improve the effect of genetic characteristics

Inactive Publication Date: 2018-10-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For gramineous plants such as rice, wheat, rape, and corn, if it encounters rain and high temperature during harvest, there will be premature germination on the parent plant in the field before the seeds are harvested, and the embryos will consume part of their nutrients and storage substances, causing the seeds to be eaten. The decline in quality and storage quality seriously deteriorates the quality, seed value and storability of seeds, which will cause greater losses to agricultural production

Method used

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  • Application of myb37 protein and its coding gene in regulating plant seed germination
  • Application of myb37 protein and its coding gene in regulating plant seed germination
  • Application of myb37 protein and its coding gene in regulating plant seed germination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, the acquisition and identification of MYB37 transgenic plants

[0050] The MYB37 gene involved in this example is derived from Arabidopsis thaliana, and its sequence in the Arabidopsis genome is shown in sequence 1 in the sequence table, and sequence 1 consists of 1793 nucleotides, which is the The sequence of the MYB37 gene in the Arabidopsis genome, wherein the 236-327 and 458-854 positions are all intron sequences; the cDNA sequence of the MYB37 gene is shown in sequence 2 in the sequence table, and the sequence 2 consists of 1304 nucleosides The acid composition is the cDNA sequence of the MYB37 gene, wherein the 100th-1089th position is the coding sequence (ORF); both sequence 1 and sequence 2 encode the protein shown in sequence 3 in the sequence list, and sequence 3 consists of 329 amino acid residues composition.

[0051] 1. Construction of recombinant expression vector pCAMBIA-1300-221-MYB37

[0052] The total RNA of Arabidopsis wild-type (Col-0...

Embodiment 2

[0087] Embodiment 2, MYB37 transgenic plant seed germination test analysis

[0088] ABA plays an important regulatory role in promoting seed dormancy and inhibiting seed germination. Genetic studies have shown that chromatin remodeling, histone methylation and deacetylation during seed germination are all regulated by ABA. With the increase of exogenous ABA concentration, the germination rate of Arabidopsis wild-type (Col-0 ecotype) seeds gradually decreased. Statistical seed germination experiment is one of the important methods to study ABA signal transduction.

[0089] With Arabidopsis wild type (Col-0 ecotype), the single-copy inserted T obtained in Example 1 3 The homozygous MYB37 transgenic lines OE1 and OE6 were used as experimental materials. The seeds of each experimental material were sown on MS medium containing different concentrations of ABA (0 μM, 0.5 μM, 1 μM and 3 μM) (80-100 seeds were sown for each experimental material). After 3 days of low-temperature s...

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Abstract

The invention discloses application of MYB37 protein and coding genes thereof to control of plant seed germination. Specifically, the protein (namely the MYB37 protein) formed by an amino acid sequence shown as the sequence 3 in a sequence table is applied to (a1) improvement in vivipary resistance of a plant or (a2) breeding of a plant variety with improved vivipary resistance. According to the application, the germination rate of plant seeds can be controlled by regulating the expression level of the MYB37, and high-expression vivipary-resistant transgenic crops containing MYB37 genes are obtained by means of gene engineering. The application meets the sustainable agricultural development demand and has important practical value and market prospect for improving eating quality, storage quality and the like of seeds.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of MYB37 protein and its coding gene in regulating plant seed germination. Background technique [0002] The phytohormone abscisic acid (ABA) was discovered in the 1960s and got its name because it can promote the shedding of plant leaves. ABA plays an important role in all stages of plant growth and development, including seed dormancy, seed germination, seedling growth, stomatal movement, and transition from vegetative growth to reproductive growth. abiotic stresses such as drought, high salinity, cold and mechanical injury, and biotic stresses such as pests and diseases. [0003] In the past 30 years, great achievements have been made in the screening and identification of ABA receptors and their signal transduction functional components in plants, the biological metabolism and transport of ABA, the construction of ABA signal transduction pathway models, and the cro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10A01H6/20
CPCC07K14/415C12N15/8267
Inventor 张大鹏于泳涛王小芳
Owner TSINGHUA UNIV
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