Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for increasing plant tolerance to NACL by downregulating Pab2 and Pab8

A plant and salt tolerance technology, applied in the biological field, can solve the problems of less PABP gene research and achieve the effect of improving genetic characteristics

Inactive Publication Date: 2018-11-06
TSINGHUA UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The research on PABP gene is mainly concentrated in yeast and animals, and their primary structure and gene structure are highly conserved, while the research on PABP gene in higher plants is less

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for increasing plant tolerance to NACL by downregulating Pab2 and Pab8
  • A method for increasing plant tolerance to NACL by downregulating Pab2 and Pab8
  • A method for increasing plant tolerance to NACL by downregulating Pab2 and Pab8

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. Identification and expression analysis of pab2-1 mutant, pab8-1 mutant and pab2-1pab8-1 double mutant

[0061] 1. Identification of pab2-1 mutant, pab8-1 mutant and pab2-1pab8-1 double mutant

[0062] according to figure 1 According to the combination of primers, the pab2-1pab8-1 double mutation homozygous plants were identified by PCR technique. In addition, homozygous plants of pab2-1 mutant and pab8-1 mutant were identified by the same method.

[0063] The primer sequences used are as follows:

[0064] pab2-1LP: 5'-ATTCGAAAGTGTCAAACACGC-3'(Left primer, LP)

[0065] pab2-1RP: 5'-TAATAAAAATGTTGCCAGCGC-3'(Right primer, RP)

[0066] pab8-1LP: 5'-ATCAACAACAGCTTGTACCGG-3'(Left primer, LP)

[0067] pab8-1RP: 5'-CAGTCTGTTTTCGGAAGCAAG-3'(Right primer, RP)

[0068] LBb1: 5'-GCGTGGACCGCTTGCTGCAACT-3'(Left border primer, LB)

[0069] 2. Analysis of corresponding gene expression in pab2-1 mutant, pab8-1 mutant and pab2-1pab8-1 double mutation

[0070] Total RNA...

Embodiment 2

[0092] Example 2, analysis test of pab2-1pab8-1 double mutant tolerance to NaCl

[0093] NaCl can inhibit the growth of plant seedlings. With the increase of exogenous NaCl concentration, the inhibition of Arabidopsis wild type (Col-0 ecotype) was enhanced, and the growth of its main root and leaves were affected to varying degrees. The experiment was repeated three times.

[0094] Arabidopsis wild type (Col-0 ecotype) and pab2-1pab8-1 double mutant plants were used as experimental materials. Seeds of each experimental material were sown on MS plates without NaCl, stratified at 4°C for 3 days, then placed in a light incubator for 72 hours, and then the newly germinated seedlings of each experimental material were transferred to MS plates containing different concentrations of NaCl. On the medium (0mM, 50mM, 100mM, 150mM and 200mM), observe the growth of Arabidopsis seedlings after vertical growth for 9 days and take pictures.

[0095] The result is as Figure 5 As shown, on...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving NaCl tolerance of plants by reducing PAB2 and PAB8. The method specifically provides any application of protein 1 and protein 2 which serve as targets as follows: a1) salt tolerance of the plants is improved; a2) plant varieties with salt tolerance improved are selected and bred, wherein the protein 1 is PAB2 protein, and the protein 2 is PAB8 protein. Research finds that NaCl tolerance of gene double knockout mutants of PAB2 and PAB8 obtained by an arabidopsis biological research center is enhanced. Accordingly, PAB2 and PAB8 can be used for adjusting the NaCl tolerance of the plants. Besides, genetically modified crops low in PAB2 and PAB8 expression and tolerant to salt can be obtained trough a genetic engineering means (RNAi technology). The method meets the requirement for sustainable agricultural development and has important practical value and market prospects in the aspects of research of plant NaCl tolerance molecular mechanism, improvement of genetic characteristics, culture of efficient salt-tolerant new varieties and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a method for improving plant tolerance to NaCl by down-regulating PAB2 and PAB8. Background technique [0002] As the world's population continues to grow, the human demand for food is increasing. Salt stress is one of the main factors affecting crop yield. Salt stress mainly causes damage to plants through sodium chloride as a stress. The high concentration of sodium ions in the soil will directly affect the photosynthesis, protein synthesis and energy metabolism of plants, etc., resulting in reduced plant germination rate, stunted growth, inhibited growth and differentiation of organs, slowed metabolism, etc., ultimately affecting crop yield. So far, the global agricultural land affected by salt stress is as high as 1 / 4 to 1 / 3. In addition, an assessment suggests that more than 10 million hectares of land have to be abandoned each year due to high salinization. [0003] Under the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29A01H5/00A01H6/20
CPCY02A40/135
Inventor 张大鹏卢艳芬于泳涛王晓静王小芳
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com