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Application of MYB28 protein and encoding gene thereof in regulation and control of tolerance of plants on ABA

A technique for encoding genes and transgenic plants, which is applied in the application field of MYB28 protein and its encoding genes in regulating plant tolerance to ABA, and can solve agricultural production losses, consumption of nutrients and storage materials, deterioration of seed quality, and seed value And storability and other issues, to improve the edible quality of seeds, improve the effect of genetic characteristics

Inactive Publication Date: 2015-05-06
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For gramineous plants such as rice, wheat, rape, and corn, if it encounters rain and high temperature during harvest, there will be premature germination on the parent plant in the field before the seeds are harvested, and the embryos will consume part of their nutrients and storage substances, causing the seeds to be eaten. The decline in quality and storage quality seriously deteriorates the quality, seed value and storability of seeds, which will cause greater losses to agricultural production

Method used

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  • Application of MYB28 protein and encoding gene thereof in regulation and control of tolerance of plants on ABA
  • Application of MYB28 protein and encoding gene thereof in regulation and control of tolerance of plants on ABA
  • Application of MYB28 protein and encoding gene thereof in regulation and control of tolerance of plants on ABA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1, the acquisition and identification of MYB28 transgenic plants

[0062] The MYB28 gene involved in this example is derived from Arabidopsis thaliana, and its sequence in the Arabidopsis genome is shown in sequence 1 in the sequence table, sequence 1 consists of 1647 nucleotides, of which the 136th -215 and 346-485 are intron sequences; the cDNA sequence of the MYB28 gene is shown in sequence 2 in the sequence table, and sequence 2 is composed of 1427 nucleotides, wherein the 3-1103 is the coding sequence (ORF); Both Sequence 1 and Sequence 2 encode the protein shown in Sequence 3 in the Sequence Listing, and Sequence 3 consists of 366 amino acid residues.

[0063] 1. Construction of recombinant expression vector pCAMBIA-1300-221-MYB28

[0064] The total RNA of Arabidopsis wild-type (Col-0 ecotype) was extracted, and cDNA was obtained after reverse transcription. Using the obtained cDNA as a template, PCR amplification was carried out with primers 1 and 2....

Embodiment 2

[0105] Example 2, MYB28 transgenic plant tolerance analysis test to ABA

[0106] 1. The effect of ABA on the seedling growth of each genetic material of MYB28

[0107] ABA can inhibit the growth of plant seedlings. With the increase of exogenous ABA concentration, the inhibition of Arabidopsis wild type (Col-0 ecotype) was enhanced, and the growth of its main root and leaves would be affected to varying degrees. The experiment was repeated three times.

[0108] The single-copy inserted T 3 The homozygous MYB28 transgenic lines OE1 and OE2, and the control plants obtained in Example 1 transformed into the pCAMBIA-1300-221 empty vector were used as experimental materials. The seeds of each experimental material were sown on MS medium containing different concentrations of ABA (0 μM, 0.8 μM and 1.4 μM) (80-100 seeds were sown for each experimental material). After low-temperature stratification at 4°C for 3 days, they were moved into light incubators, and the growth of Arabido...

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Abstract

The invention discloses an application of an MYB28 protein and an encoding gene thereof in regulation and control of tolerance of plants on ABA. The application provided by the invention is an application of the protein as shown in a sequence 3 in the following aspects: (a1) regulation and control of the tolerance of plants on ABA; (a2) breeding of a plant variety which is lowered or increased in the tolerance on ABA; (a3) regulation and control of vivipary resistance of plants; and (a4) breeding of a plant variety which is increased or lowered in the vivipary resistance. The MYB28 protein can be applied to obtaining of MYB28 low-expressed and herbicide-resistant transgenic crops employing plant hormone ABA as a selective herbicide through genetic engineering means, so as to achieve selective weeding; the germination rate of plant seeds can also be controlled by adjusting the expression level of the MYB28; MYB28 gene high-expressed and vivipary-resistant transgenic crops can be obtained through the genetic engineering means; the application conforms to the requirements of sustainable agriculture development, and has important practical value and market prospects in the aspects such as development of green, environment-friendly and pollution-free weeding methods and improvement of eating quality and storage quality of seeds.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a MYB28 protein and its coding gene in regulating plant tolerance to ABA. Background technique [0002] Abscisic acid (ABA) is one of the important hormones in plants. It was discovered as early as the early 1960s. It is named for its function of promoting plant leaf shedding. The plant hormone abscisic acid (ABA) plays an important role in all stages of plant growth and development, including seed dormancy, germination, seedling growth, stomatal movement, and the transition from vegetative growth to reproductive growth. At the same time, ABA plays an important role in the response of plants to various external adversity stresses, such as abiotic stresses such as drought, cold, salt and mechanical damage, and biotic stresses such as pests and diseases. Since plants are sessile organisms, when encountering adverse environments, they cannot move their positions to avo...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01G7/06
CPCA01G13/00C07K14/415C12N15/8293
Inventor 张大鹏于泳涛王小芳
Owner TSINGHUA UNIV
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