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Application of eifiso4g2 protein in regulation of plant tolerance to ABA

A tolerance and protein technology, applied in the fields of application, plant peptides, plant products, etc., can solve the problems of seed edible quality, storage quality decline, consumption of nutrients and storage substances, agricultural production loss, etc.

Inactive Publication Date: 2021-02-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fetal germination consumes part of its nutrients and storage substances, resulting in a decline in the edible quality and storage quality of the seeds, causing greater losses to agricultural production

Method used

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  • Application of eifiso4g2 protein in regulation of plant tolerance to ABA
  • Application of eifiso4g2 protein in regulation of plant tolerance to ABA
  • Application of eifiso4g2 protein in regulation of plant tolerance to ABA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, the acquisition and identification of transgenic plants

[0065] 1. Obtaining of Mutant ei4g2-1

[0066] Seeds of Arabidopsis thaliana with the number SALK_076633 (the background is Arabidopsis ecotype Columbia, a T-DNA insertion mutant) were commercially purchased from the Arabidopsis Biological Research Center, and grown into plants. Genomic DNA was extracted from the plants at the seedling stage, and PCR identification was carried out with the primer pair consisting of ei4g2-1 LP and ei4g2-1 RP and the primer pair consisting of LBb1.3 and ei4g2-1 RP, respectively. If the amplified product is not detected by the primer pair composed of ei4g2-1 LP and ei4g2-1 RP, and the amplified product can be detected by the primer pair composed of LBb1.3 and ei4g2-1 RP, it proves that the plant is two Chromosomally identical homozygous mutants. Homozygous mutants were sequenced for verification.

[0067] ei4g2-1 LP: 5'-ATGCAACAACAAGGTGAACC-3';

[0068] ei4g2-1 RP:...

Embodiment 2

[0092] Embodiment 2, the tolerance of plant to ABA

[0093] 1. Seed Germination Experiment

[0094] ABA plays an important regulatory role in promoting seed dormancy and inhibiting seed germination. Genetic studies have shown that chromatin remodeling, histone methylation and deacetylation during seed germination are all regulated by ABA. With the increase of exogenous ABA concentration, the germination rate of wild-type Arabidopsis seeds will gradually decrease. Statistical seed germination experiment is one of the important methods to study ABA signal transduction.

[0095] The tested seeds were: seeds of Arabidopsis thaliana ecotype Colombia, and seeds of mutant ei4g2-1.

[0096] The first group: sow the tested seeds on the MS medium plate, stratify at 4°C for 3 days, then transfer to the light incubator for cultivation, and culture in the light incubator for 36 hours, 48 ​​hours or 72 hours, then count Seed germination rate.

[0097] The second group: Sow the tested s...

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Abstract

The invention discloses the application of the eIFiso4G2 protein in regulating the tolerance of plants to ABA. The present invention claims to protect the application of substances used to inhibit the expression of specific genes in plants: regulating the tolerance of plants to abscisic acid; breeding plant varieties with reduced tolerance to abscisic acid; regulating the embryonic resistance of plants; breeding A plant variety with increased fetal germination resistance; the specific gene is the gene encoding eIFiso4G2 protein. The present invention finds that the eIFiso4G2 protein is an important negative regulator in ABA signal transduction, and knocking out the eIFiso4G2 gene can significantly reduce the tolerance of plants to ABA. The invention meets the requirement of sustainable agricultural development, and has important practical value and market prospect for cultivating new varieties with high efficiency and tolerance to ABA and adversity stress.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of eIFiso4G2 protein in regulating plant tolerance to ABA. Background technique [0002] Abscisic acid (ABA) is one of the important hormones in plants. It plays an important role in all stages of plant growth and development, including seed dormancy, germination, seedling growth, stomatal movement, and transition from vegetative growth to reproductive growth. Wait for the process. At the same time, ABA plays an important role in the response of plants to various external adversity stresses, such as abiotic stresses such as drought, low temperature, high temperature, salt, mechanical damage, and biotic stresses such as pests and diseases. Therefore, the elucidation of the molecular mechanism of the plant ABA signal transduction pathway is of great significance for regulating plant growth, improving crop quality, improving genetic characteristics, and prom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C07K19/00C12N15/82A01H5/00A01H6/20
CPCC07K14/415C07K2319/00C12N15/8293
Inventor 张大鹏马宇毕超
Owner TSINGHUA UNIV
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