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Application of myb99 protein and its coding gene in regulating plant seed germination

A technology of encoding genes and transgenic plants, applied in the fields of application, plant peptides, plant products, etc., can solve the problem of seed edible quality, storage quality decline, consumption of seed nutrition and storage substances, deterioration of seed quality, seed value and storage resistance To achieve the effect of improving the edible quality and storage quality of seeds and improving the genetic characteristics

Inactive Publication Date: 2018-10-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For gramineous plants such as rice, wheat, rape, and corn, if there is rain and high temperature during harvest, there will be premature germination on the parent plant in the field before the seeds are harvested. The embryonic germination will consume part of the nutrients and storage substances of the seeds, causing the seeds to germinate. The food quality and storage quality decline, seriously deteriorating the quality, seed value and storability of seeds, which will cause greater losses to agricultural production

Method used

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  • Application of myb99 protein and its coding gene in regulating plant seed germination
  • Application of myb99 protein and its coding gene in regulating plant seed germination
  • Application of myb99 protein and its coding gene in regulating plant seed germination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Acquisition and identification of MYB99 transgenic plants

[0050] The MYB99 gene involved in this example is derived from Arabidopsis thaliana, and its sequence in the Arabidopsis thaliana genome is as shown in sequence 1 in the sequence listing, which consists of 1218 nucleotides, of which the 435th Position -598 is an intron sequence; the cDNA sequence of the MYB99 gene is shown in sequence 2 in the sequence listing, which consists of 1054 nucleotides, of which positions 148-885 are the coding sequence (ORF); sequence 1 Both sequence 2 and sequence 2 encode the protein shown in sequence 3 in the sequence listing, which consists of 245 amino acid residues.

[0051] 1. Construction of recombinant expression vector pCAMBIA-1300-221-MYB99

[0052] The total RNA of Arabidopsis wild type (Col-0 ecotype) was extracted, and cDNA was obtained after reverse transcription. Using the obtained cDNA as a template, PCR amplification was carried out with primer 1 and primer 2....

Embodiment 2

[0088] Example 2. Seed germination analysis test of MYB99 transgenic plant

[0089] 1. The effect of ABA on seed germination of MYB99 overexpression lines

[0090] ABA plays an important regulatory role in promoting seed dormancy and inhibiting seed germination. Genetic studies have shown that the processes of chromatin reconstruction, histone methylation and deacetylation that occur during seed germination are all regulated by ABA. As the concentration of exogenous ABA increases, the germination rate of Arabidopsis wild-type (Col-0 ecotype) seeds will gradually decrease. Statistical seed germination experiments are one of the important methods to study ABA signal transduction. The experiment was repeated three times.

[0091] With Arabidopsis wild type (Col-0 ecotype) and the single copy inserted T obtained in Example 1 3 Generation homozygous MYB99 transgenic lines OE1, OE2, OE5 and OE6 were experimental materials. The seeds of each experimental material were sown on MS medium ...

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Abstract

The invention discloses application of MYB99 protein and coding genes thereof to control of plant seed germination. Specifically, the protein (namely the MYB99 protein) formed by an amino acid sequence shown as the sequence 3 in a sequence table is applied to (a1) control of vivipary resistance of a plant or (a2) breeding of a plant variety with improved vivipary resistance. According to the application, the germination rate of plant seeds can be controlled by regulating the expression level of the MYB99, and high-expression vivipary-resistant transgenic crops containing MYB99 genes are obtained by means of gene engineering. The application meets the sustainable agricultural development demand and has important practical value and market prospect for improving eating quality, storage quality and the like of seeds.

Description

Technical field [0001] The invention belongs to the field of biotechnology and relates to the application of MYB99 protein and its coding gene in regulating the germination of plant seeds. Background technique [0002] The plant hormone abscisic acid (ABA) was discovered as early as the 1960s, and it was named for its ability to promote plant leaf shedding. ABA is an important plant hormone. It plays an important role in the growth and development of plants such as seed dormancy, seed germination, seedling growth, stomatal movement, and conversion from vegetative growth to reproductive growth. At the same time, ABA plays an important role in the plant's response to various external adversities. It also plays an important role in stress response, such as abiotic stresses such as drought, high salt, and cold, and biological stresses such as pests and diseases. [0003] In the past 20 years, many important advances have been made in the research of ABA signal transduction. A large nu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10A01H6/20
CPCC07K14/415C12N15/8267
Inventor 张大鹏于泳涛王小芳
Owner TSINGHUA UNIV
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