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Method for improving tolerance of plant to droughts through down-regulation of expression of eIFiso4G1 gene and eIFiso4G2 gene

A technology of transgenic plants and plants, applied in the fields of botanical equipment and methods, chemical equipment and methods, biochemical equipment and methods, etc., can solve the problems of difficult stress tolerance traits, etc.

Inactive Publication Date: 2019-08-13
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is relatively difficult to cultivate and improve stress tolerance traits by traditional breeding techniques, and it is difficult to obtain excellent drought-resistant varieties

Method used

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  • Method for improving tolerance of plant to droughts through down-regulation of expression of eIFiso4G1 gene and eIFiso4G2 gene
  • Method for improving tolerance of plant to droughts through down-regulation of expression of eIFiso4G1 gene and eIFiso4G2 gene
  • Method for improving tolerance of plant to droughts through down-regulation of expression of eIFiso4G1 gene and eIFiso4G2 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1. Acquisition and identification of mutants

[0062] First, the acquisition of mutant ei4g1-1

[0063] The Arabidopsis thaliana seeds with the serial number SALK_098730 (the background is the Colombian ecotype Arabidopsis thaliana, which is a T-DNA insertion mutant) were purchased from the Arabidopsis Biological Research Center and cultivated into plants. The genomic DNA of the plants at the seedling stage was extracted and identified by PCR with the primer pair consisting of ei4g1-1LP and ei4g1-1RP and the primer pair consisting of LBb1.3 and ei4g1-1RP, respectively. If the primer pair composed of ei4g1-1LP and ei4g1-1RP does not detect the amplification product, and the primer pair composed of LBb1.3 and ei4g1-1RP can detect the amplification product, it proves that the plant is the same as the two chromosomes Homozygous mutants. Homozygous mutants were verified by sequencing.

[0064] ei4g1-1LP:5'-TCAAGGGCAAACATATCATCC-3';

[0065] ei4g1-1RP:5'-TTTTGACTT...

Embodiment 2

[0106] Example 2. Resistance of plants to drought stress

[0107] 1. ABA promotes stomatal closure and inhibits stomatal opening experiments

[0108] ABA is an important signaling molecule for plants to resist external stress. Under drought conditions, ABA can promote stomatal closure to reduce water loss by regulating ion channels in stomatal guard cells. Calcium ions, protein kinases, phosphatases, etc. are all involved in ABA-mediated signal transduction of guard cells and signal regulation of ABA-related anti-stress genes. Therefore, the ABA-induced stomatal movement experiment was used to detect whether eIFiso4G1 and eIFiso4G2 proteins play a role in the process of ABA-regulated plant drought stress response.

[0109] Epidermal strip buffer: MES-KOH (pH 6.15) containing 50 mM KCl, 10 mM.

[0110] The light intensity for incubation under cold light source was 200 μM Photons m -2 s -1 .

[0111] 1. ABA inhibits stomatal opening experiment

[0112] The tested plants a...

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Abstract

The invention discloses a method for improving the tolerance of a plant to droughts through down-regulation of expression of an eIFiso4G1 gene and an eIFiso4G2 gene. Application of the method in inhibiting expression substances of a specific gene A and a specific gene B in the plant comprises regulation and control over drought tolerance of the plant and seed selection for plant varieties of the improved drought tolerance. The specific gene A is a gene for encoding eIFiso4G1 protein; the specific gene B is a gene for encoding eIFiso4G2 protein. The inventor discovers that through knockout of the eIFiso4G1 gene and the eIFiso4G2 gene, the tolerance of the plant to droughts can be significantly improved. The method accords with the demands of the sustainable agricultural development and hasimportant practical value and an important market prospect in the aspects of studying the drought-tolerance molecule mechanism of the plant, improving the hereditary characters of the plant, improvingthe stress resistance of the plant to natural climatic disasters and bad soil environments, cultivating new efficient drought-tolerance varieties and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving drought tolerance of plants by down-regulating eIFiso4G1 gene and eIFiso4G2 gene. Background technique [0002] Terrestrial plants may be affected by a variety of external stresses during their entire growth and development stages. Among them, abiotic stresses such as early dryness, salinity, low temperature, and high temperature, as well as biotic stresses such as pests and diseases, significantly affect plant growth and crop yield. Research on plant stress resistance has always been a hot spot in the field of botany. Traditional breeding techniques are relatively difficult to cultivate and improve stress-tolerant traits, and can not obtain excellent drought-resistant varieties. With the development of molecular biology technology and the in-depth study of the molecular mechanism of plant stress resistance, great progress has been made in plant st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K19/00C12N15/82C12N15/29A01H5/00A01H6/20
CPCC07K14/415C07K2319/00C12N15/8273
Inventor 张大鹏马宇毕超
Owner TSINGHUA UNIV
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