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Application of eifiso4g1 protein in regulation of plant drought resistance

A drought resistance and plant technology, applied in the fields of application, plant peptides, plant products, etc., can solve the problems of difficult stress tolerance traits, achieve the effect of improving genetic characteristics and improving stress resistance

Inactive Publication Date: 2020-08-04
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is relatively difficult to cultivate and improve stress tolerance traits by traditional breeding techniques, and it is difficult to obtain excellent drought-resistant varieties

Method used

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  • Application of eifiso4g1 protein in regulation of plant drought resistance
  • Application of eifiso4g1 protein in regulation of plant drought resistance
  • Application of eifiso4g1 protein in regulation of plant drought resistance

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, the acquisition and identification of mutants

[0049] 1. The acquisition of mutant ei4g1-1

[0050] Seeds of Arabidopsis thaliana with the number SALK_098730 (the background is Arabidopsis ecotype Columbia, a T-DNA insertion mutant) were purchased from the Arabidopsis Biological Research Center and grown into plants. The genomic DNA of the plants at the seedling stage was extracted, and the primer pairs composed of ei4g1-1LP and ei4g1-1RP and the primer pairs composed of LBb1.3 and ei4g1-1RP were used for PCR identification. If the amplified product is not detected by using the primer pair composed of ei4g1-1LP and ei4g1-1RP, and the amplified product can be detected by using the primer pair composed of LBb1.3 and ei4g1-1RP, it proves that the plant has the same two chromosomes homozygous mutant. Homozygous mutants were sequenced for verification.

[0051] ei4g1-1LP: 5'-TCAAGGGCAAACATATCATCC-3';

[0052] ei4g1-1RP: 5'-TTTTGACTTCACGTTTCCGTC-3';

[005...

Embodiment 2

[0076] Embodiment 2, the resistance of plant to drought stress

[0077] 1. ABA promotes stomatal closure and inhibits stomatal opening experiment

[0078] ABA is an important signaling molecule for plants to resist external adversity stress. Under drought conditions, ABA can promote stomatal closure to reduce water loss by regulating ion channels in stomatal guard cells. Calcium ions, protein kinases, and phosphatases are all involved in ABA-mediated guard cell signal transduction and the signal regulation of ABA-related stress resistance genes. Therefore, ABA-induced stomatal movement experiments were used to detect whether eIFiso4G1 protein plays a role in the process of ABA regulating plant drought stress response.

[0079] Strip buffer: MES-KOH (pH 6.15) containing 50 mM KCl and 10 mM.

[0080] The light intensity for culturing under a cold light source was 200 μM Photons m -2 the s -1 .

[0081] 1. ABA inhibits stomatal opening experiment

[0082] The tested plants...

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Abstract

The invention discloses application of eIFiso4G1 protein to regulation and control of the drought resistance of plants. The application required for protecting substances used for inhibiting specificgene expression in the plants comprises the following steps of regulating and controlling the drought resistance of the plants; breeding plant varieties with improved drought resistance. The inventionalso provides a method for preparing transgenic plants. The method comprises the following steps that the specific gene expression in receiver plants is inhibited to obtain the transgenic plants; compared with the receiver plants, the drought resistance of the transgenic plants is improved. A specific gene is a gene encoding the eIFiso4G1 protein. The inventor finds that the drought tolerance ofthe plants can be obviously enhanced by knocking out an eIFiso4G1 gene. The sustainable agriculture development requirement is conformed; important practical values and market prospects are realized in aspects of studying the plant drought resistant molecular mechanism; improving the hereditary character, improving the stress resistance of the plants on natural climatic disasters and poor soil environment, culturing new efficient drought resistant varieties and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of eIFiso4G1 protein in regulating the drought resistance of plants. Background technique [0002] Terrestrial plants may be affected by a variety of external adversity stresses throughout their growth and development stages. Among them, abiotic stresses such as drought, salinity, low temperature, and high temperature, and biotic stresses such as pests and diseases significantly affect plant growth and crop yield. The research on plant stress resistance has always been a hot spot in the field of botany. It is relatively difficult to cultivate and improve stress-tolerant traits by traditional breeding techniques, and it is difficult to obtain excellent drought-resistant varieties. With the development of molecular biology technology and the in-depth study of the molecular mechanism of plant stress resistance, the genetic engineering of plant stress resista...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/20
CPCC07K14/415C12N15/8273
Inventor 张大鹏毕超马宇
Owner TSINGHUA UNIV
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