Method for promoting rapid propagation of arbuscular mycorrhizal fungal spores

A technology of arbuscular mycorrhizal fungi and spores, which is applied in the field of microbial cultivation, can solve the problems of being easily contaminated by miscellaneous bacteria, low spore content, and low quality of bacterial preparations, and achieves avoidance of miscellaneous bacteria pollution, high spore content, and high quality of bacterial preparations. Good results

Inactive Publication Date: 2018-01-16
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the pot culture method is the main method for the propagation of commercial AMF fungi at present. Its advantages are simple operation and reliable method. months, the culture cycle is longer, and the spore content is less; open management is prone to contamination by bacteria, and the quality of the bacterial agent is low, which is difficult to meet the market demand

Method used

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  • Method for promoting rapid propagation of arbuscular mycorrhizal fungal spores
  • Method for promoting rapid propagation of arbuscular mycorrhizal fungal spores
  • Method for promoting rapid propagation of arbuscular mycorrhizal fungal spores

Examples

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Effect test

Embodiment 1

[0033] The method for promoting the rapid propagation of arbuscular mycorrhizal fungus spores in this embodiment is carried out in the following steps:

[0034] (1) Mix vermiculite, zeolite, pumice and peat into a substrate at a volume ratio of 3:3:3:1, dry heat sterilization at 160°C for 4 hours, and obtain arbuscular mycorrhizal fungus culture substrate, whose basic physical and chemical properties are as follows : Organic matter 14.0g / kg, total nitrogen 1.16g / kg, total potassium 1.11g / kg, total phosphorus 30mg / kg.

[0035] (2) Select corn, marigold and clover as host plants, select plump and uniform corn seeds, and adopt a concentration of 1% H 2 o 2 Disinfect for 10 minutes, then rinse with distilled water and soak for 4 hours in an incubator at 28°C to accelerate germination; the concentration of clover and marigold seeds is 1% H 2 o 2 After 10 minutes of disinfection, the seeds can be sown. Use a flower pot with a diameter of 21 cm and a height of 18 cm as a cultivat...

Embodiment 2

[0042]The difference between this embodiment and Example 1 is that in step (3), it is set to simultaneously add spermidine and arbuscular mycorrhizal fungi to treat, only inoculate arbuscular mycorrhizal fungi, only add spermidine to treat, and not add arbuscular mycorrhizal fungi. Mycorrhizal fungus and spermidine are handled, altogether 4 are handled; In step (4), spermidine is pressed 0.25mg / m 3 Spray evenly on the bacterial agent, other steps and parameters are the same as the first embodiment.

[0043] Determination of mycorrhizal infection rate: 1) After cleaning the root system, cut it into 1-2cm long pieces and put them in a beaker; 2) Add 10% KOH solution, treat at 90°C for 40min, pour off the liquid Finally, wash the root system with distilled water; 3) Add 2% lactic acid or 2% HCl, treat at 90°C for 20 minutes, and pour off the liquid; 4) Add 0.05% trypan blue dye solution, dye at 90°C for 30 minutes, cool for at least 30min; 5) Pour off the dye solution, add a dec...

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Abstract

The invention discloses a method for promoting rapid propagation of arbuscular mycorrhizal fungal spores. According to the method, an arbuscular mycorrhizal fungal inoculant can be obtained after synthetic hormone GR24 or spermidine is added exogenously and is cultivated under a room temperature condition for 3 months by adopting an arbuscular mycorrhizal fungi potted cultivation technology. The method has the advantages that the materials are easily obtained, the operation is simple, the cost is comparatively low, the culture cycle is short, the mycorrhizal infection rate is high, and the spore density of the obtained inoculant is comparatively high; a nin the method, a cultivation vessel is simple, and the risk of mixed fungi pollution is avoided, and therefore, the quality of the inoculant is comparatively high.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and relates to a method for rapid propagation of arbuscular mycorrhizal fungus spores. Background technique [0002] Arbuscular mycorrhizal fungi (AMF) are a class of microorganisms that can form mutualistic symbionts with the roots of most plants. They are the most widely distributed mycorrhizal species most closely related to agricultural and forestry production. AMF has a series of beneficial physiological effects. Studies have shown that AMF can form a huge hyphae network system in the plant root system, enhance the host plant's ability to absorb nitrogen, phosphorus, potassium and other elements in the soil, significantly improve the nutritional status of the plant, and promote plant health. Growth, improve the stress resistance of plants. Therefore, AMF has broad application prospects in the fields of agricultural and forestry production and landscaping. The growth-promotin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/645
Inventor 孙锦刘铭铭郭世荣仇紫岩吴亚胜
Owner NANJING AGRICULTURAL UNIVERSITY
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