Application of eif4g protein in regulation of plant tolerance to ABA

A tolerance, plant technology, applied in applications, plant peptides, plant products, etc., can solve the problems of seed eating quality, storage quality decline, consumption of nutrients and storage substances, loss of agricultural production, etc.

Inactive Publication Date: 2020-08-04
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fetal germination consumes part of its nutrients and storage substances, resulting in a decline in the edible and storage quality of the seeds, causing greater losses to agricultural production

Method used

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  • Application of eif4g protein in regulation of plant tolerance to ABA
  • Application of eif4g protein in regulation of plant tolerance to ABA
  • Application of eif4g protein in regulation of plant tolerance to ABA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, the acquisition and identification of mutants

[0065] 1. Obtaining of mutant e4g-2

[0066] Seeds of Arabidopsis thaliana with the number SALK_136398 (the background is Arabidopsis ecotype Columbia, a T-DNA insertion mutant) were commercially purchased from the Arabidopsis Biological Research Center, and grown into plants. The genomic DNA of the plants at the seedling stage was extracted, and the primer pairs composed of e4g-2LP and e4g-2RP and the primer pairs composed of LBb1.3 and e4g-2RP were used for PCR identification. If the amplified product is not detected by using the primer pair composed of e4g-2LP and e4g-2RP, and the amplified product can be detected by using the primer pair composed of LBb1.3 and e4g-2RP, it proves that the plant has the same two chromosomes homozygous mutant. Homozygous mutants were sequenced for verification.

[0067] e4g-2LP: 5'-AACCTTCTTCGTTCTCTTCGC-3';

[0068] e4g-2RP: 5'-CTGACAACGCCTTAAACGAAG-3';

[0069] LBb1.3:...

Embodiment 2

[0092] Embodiment 2, the tolerance of plant to ABA

[0093] 1. Seed Germination Experiment

[0094] ABA plays an important regulatory role in promoting seed dormancy and inhibiting seed germination. Genetic studies have shown that chromatin remodeling, histone methylation and deacetylation during seed germination are all regulated by ABA. With the increase of exogenous ABA concentration, the germination rate of wild-type Arabidopsis seeds will gradually decrease. Statistical seed germination experiment is one of the important methods to study ABA signal transduction.

[0095] The tested seeds were: seeds of Arabidopsis thaliana ecotype Colombia, and seeds of mutant e4g-2.

[0096] The first group: sow the tested seeds on the MS medium plate, stratify at 4°C for 3 days, then transfer to the light incubator for cultivation, and culture in the light incubator for 36 hours, 48 ​​hours or 72 hours, then count Seed germination rate.

[0097] The second group: Sow the tested see...

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Abstract

The invention discloses application of eIF4G protein to regulation and control of the tolerance of plants on ABA (abscisic acid). The application required for protecting substances used for inhibitingspecific gene expression in the plants comprises the following steps of regulating and controlling the tolerance of the plants on the ABA; breeding plant varieties with reduced tolerance on the ABA;regulating and controlling vivipary resistance of the plants; breeding plant varieties with increased vivipary resistance, wherein a specific gene is a gene encoding the eIF4G protein. The conditionsthat the eIF4G protein is an important negative regulator in ABA signal conduction, and the tolerance of the plants on the ABA can be obviously reduced by knocking out the eIF4G gene are found. The sustainable agriculture development requirement is conformed; important practical values and market prospects are realized in aspects of culturing new efficient ABA and adversity stress resistant varieties and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of eIF4G protein in regulating plant tolerance to ABA. Background technique [0002] Abscisic acid (ABA) is one of the important hormones in plants. It plays an important role in all stages of plant growth and development, including seed dormancy, germination, seedling growth, stomatal movement, and transition from vegetative growth to reproductive growth. Wait for the process. At the same time, ABA plays an important role in the response of plants to various external adversity stresses, such as abiotic stresses such as drought, low temperature, high temperature, salt, mechanical damage, and biotic stresses such as pests and diseases. Therefore, the elucidation of the molecular mechanism of the plant ABA signal transduction pathway is of great significance for regulating plant growth, improving crop quality, improving genetic characteristics, and promotin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/20
CPCC07K14/415C12N15/8261
Inventor 张大鹏毕超马宇
Owner TSINGHUA UNIV
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