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Application of CPN20 protein and coding gene thereof in regulating ABA (abscisic acid) tolerance of plant

A coding gene and tolerance technology, applied in the application field of CPN20 protein and its coding gene in regulating plant tolerance to ABA, to achieve the effect of improving genetic characteristics

Inactive Publication Date: 2014-07-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanism of ABAR / CHLH-mediated signal transduction remains to be studied, and the screening of key functional components in this pathway is necessary to understand the complex mechanism of action of ABA signal transduction

Method used

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  • Application of CPN20 protein and coding gene thereof in regulating ABA (abscisic acid) tolerance of plant
  • Application of CPN20 protein and coding gene thereof in regulating ABA (abscisic acid) tolerance of plant
  • Application of CPN20 protein and coding gene thereof in regulating ABA (abscisic acid) tolerance of plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, the acquisition and identification of CPN20 transgenic plants

[0053] The CPN20 gene involved in this example is derived from Arabidopsis thaliana, and its sequence in the Arabidopsis genome is shown as sequence 1 in the sequence table. Sequence 1 consists of 1813 nucleotides, of which the 80th -339, 519-788, 971-1069, 1229-1315, 1393-1514 are 5 intron sequences; the cDNA sequence of the CPN20 gene is shown in sequence 2 in the sequence listing , Sequence 2 consists of 975 nucleotides, of which the 87th-848th is the coding sequence (ORF); both Sequence 1 and Sequence 2 encode the protein (CPN20 protein) shown in Sequence 3 in the sequence list, and Sequence 3 consists of 253 Composition of amino acid residues.

[0054] 1. Construction of recombinant expression vector pCAMBIA-1300-221-CPN20

[0055] Total RNA was extracted from Arabidopsis wild-type (Col-0 ecotype), and cDNA was obtained after reverse transcription. Using the obtained cDNA as a template...

Embodiment 2

[0120] Embodiment 2, CPN20 transgenic plant tolerance analysis test to ABA

[0121] 1. Effect of ABA on seed germination of CPN20 genetic materials

[0122] Arabidopsis wild type (Col-0 ecotype), two T-DNA insertion mutants cpn20-1 and cpn20-2 with reduced expression of CPN20 gene, T3 generation homozygous CPN20 transgenic line OE2 obtained in Example 1, OE3, OE4, OE5 and OE7, and the control plants obtained in Example 1 transformed into the pCAMBIA-1300-221 empty vector were used as experimental materials. The seeds of each experimental material were sown on MS medium containing different concentrations of ABA (0 μM, 0.5 μM, 1 μM and 3 μM) (80-100 seeds were sown for each experimental material). After low-temperature stratification at 4°C for 3 days, they were transferred to a light incubator. Record the germination number of seeds between 24h and 72h after stratification, record once every 12h, and make statistics on the results. The experiment was repeated 5 times, and t...

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Abstract

The invention discloses application of a CPN20 protein and a coding gene thereof in regulating the ABA (abscisic acid) tolerance of a plant. The application provided by the invention is particularly application of a protein composed of an amino acid sequence as shown in Sequence 3 in a sequence table in a1) or a2) below: a1) regulating the ABA tolerance of a plant; and a2) breeding ABA tolerance improved plant species. The invention can be used for using phytohormone ABA as a selective weedicide, the selectivity depends on the ABA sensitivity of the plant, and the CPN20 gene is used to regulate the ABA sensitivity or tolerance of the plant, thus obtaining CPN20 gene highly-expressed anti-weedicide transgenic crops and further realizing selective weed control. The invention meets the sustainable agricultural development requirements, and has important practical values and market prospects in the aspects of improving hereditary characters, developing green environment-friendly nuisance-free weed control methods and approaches and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a CPN20 protein and its coding gene in regulating plant tolerance to ABA. Background technique [0002] The plant hormone ABA plays an important regulatory role in various stages of plant development, including embryonic maturation, seed development and seedling growth. ABA is also a key hormone for plants to adapt to stress, such as drought, high salinity and low temperature stress. The mechanism of ABA signal transduction has been extensively studied, and many components related to ABA signal transduction have been found, including ABA receptors. The possible G protein-coupled receptor GCR2 and the GPCR-like G proteins GTG1 and GTG2 have been reported as candidate ABA plasma membrane receptors. However, whether GCR2 is involved in the regulation of ABA-regulated seed germination and post-germination growth is still controversial. GTGs are positive regulators of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
Inventor 王小芳张晓枫姜涛张大鹏
Owner TSINGHUA UNIV
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