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Application of OTS1 protein and coding genes thereof to control over ABA tolerance of plant

An encoding gene and transgenic plant technology, applied in the application field of OTS1 protein and its encoding gene in regulating plant tolerance to ABA, can solve the problem of deteriorating seed quality, seed value and storability, agricultural production loss, consumption Issues such as nutrition and storage substances

Inactive Publication Date: 2016-04-13
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For gramineous plants such as rice, wheat, rape, and corn, if it encounters rain and high temperature during harvest, there will be premature germination on the parent plant in the field before the seeds are harvested, and the embryos will consume part of their nutrients and storage substances, causing the seeds to be eaten. The decline in quality and storage quality seriously deteriorates the quality, seed value and storability of seeds, which will cause greater losses to agricultural production

Method used

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  • Application of OTS1 protein and coding genes thereof to control over ABA tolerance of plant
  • Application of OTS1 protein and coding genes thereof to control over ABA tolerance of plant
  • Application of OTS1 protein and coding genes thereof to control over ABA tolerance of plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1, the acquisition and identification of OTS1 transgenic plants

[0067]The OTS1 gene involved in this example is derived from Arabidopsis thaliana (Arabidopsisthaliana), and its sequence in the Arabidopsis genome is shown in sequence 1 in the sequence table. Sequence 1 consists of 3840 nucleotides, of which the 343rd- 605th, 649-770th, 917-1035th, 1350-1503rd, 1553-1884th, 1971-2050th, 2081-2160th, 2257-2358th, 2462-2580th , 2679-2761, 2885-3030, 3146-3283, and 3392-3477 are intron sequences; sequence 2 consists of 2016 nucleotides and is the cDNA sequence of the OTS1 gene, Positions 1-1755 are the coding sequence (ORF); Sequence 1 and Sequence 2 both encode the protein shown in Sequence 3 in the Sequence Listing, and Sequence 3 consists of 584 amino acid residues.

[0068] 1. Construction of recombinant expression vector pCAMBIA-1300-221-OTS1

[0069] The total RNA of Arabidopsis wild-type (Col-0 ecotype) was extracted, and cDNA was obtained after reverse...

Embodiment 2

[0110] Embodiment 2, each genetic material of OTS1 is to the ABA tolerance analysis test

[0111] 1. Effect of ABA on seedling growth of each genetic material of OTS1

[0112] ABA can inhibit the growth of plant seedlings. With the increase of exogenous ABA concentration, the inhibition of Arabidopsis wild type (Col-0 ecotype) was enhanced, and the growth of its main root and leaves would be affected to varying degrees. The experiment was repeated three times.

[0113] The single-copy inserted T 3 The homozygous OTS1 transgenic lines (OE31 and OE32) and the control plants obtained in Example 1 transformed into the pCAMBIA-1300-221 empty vector were used as experimental materials. Seeds of each experimental material were sown on MS plates not containing ABA, stratified at 4°C for 3 days at low temperature, placed in a light incubator for 60 hours, and then the newly germinated seedlings of each experimental material were moved to the medium containing different concentrations...

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Abstract

The invention discloses application of OTS1 protein and coding genes thereof to control over ABA tolerance of a plant. According to the application, the protein shown as the sequence 3 is applied to: (a1) control over the ABA tolerance of the plant; (a2) breeding of a plant variety with improved or reduced ABA tolerance; (a3) control over vivipary resistance of the plant; (a4) breeding of a plant variety with improved or reduced vivipary resistance. The plant hormone ABA can be used as selective herbicide, transgenic crops with low OTS1 expression and herbicide resistance can be obtained by means of genetic engineering, and selective weeding is achieved. The germination rate of plant seeds can be controlled by regulating the expression level of OTS1, and transgenic crops with high expression of OTS1 genes and vivipary resistance can be obtained by means of genetic engineering. The application meets the development demand for sustainable agriculture and has significant practical value and market prospect in exploiting green, environment-friendly and pollution-free weeding methods and other aspects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an application of OTS1 protein and its coding gene in regulating plant tolerance to ABA. Background technique [0002] Abscisic acid (ABA) is a plant hormone with a sesquiterpene structure. It was discovered in the early 1960s and is named for its function of promoting plant leaf shedding. ABA plays an important role in plants. ABA can inhibit the germination of seeds and the growth of seedlings, promote the closure of stomata, and regulate the growth and development of lateral root development, leaf senescence, and fruit ripening. In addition, ABA plays an important role in plant response to various external conditions. Adversity stress processes also play an important role, such as abiotic stresses such as drought, cold and high salinity, and biotic stresses such as pests and diseases. The elucidation of the molecular mechanism of the plant ABA signal transduction pathway is of grea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
CPCC12N15/8267C12N15/8293
Inventor 张大鹏辛琪于泳涛王小芳
Owner TSINGHUA UNIV
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