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4-terpilenol-type melaleuca alternifolia tissue culture subculture multiplication method and culture medium thereof

A subculture propagation and culture medium technology, which is applied in the field of 4-terpineol type Melaleuca alternifolia tissue culture subculture propagation method and its culture medium, can solve the problem of difficult production, germination rate of only 10%, essential oil content and ingredients are uneven

Active Publication Date: 2016-06-01
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The seeds of Melaleuca alternifolia are small, the germination rate is only 10%, and the yield is low, which is difficult to meet the needs of production
At the same time, the genetic differentiation among individual seedlings is large, and the biomass, essential oil content and components of a single plant are uneven, which restricts the improvement of essential oil production.

Method used

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  • 4-terpilenol-type melaleuca alternifolia tissue culture subculture multiplication method and culture medium thereof
  • 4-terpilenol-type melaleuca alternifolia tissue culture subculture multiplication method and culture medium thereof
  • 4-terpilenol-type melaleuca alternifolia tissue culture subculture multiplication method and culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Selection of excellent individual plants

[0024] Select excellent individual plants with high biomass and high oil content in the Melaleuca alternifolia forest, whose essential oil index reaches the international standard IS04730-2004 for "tea tree oil", its 4-terpineol content> 35%, 1,8-eucalyptus Leaf element content <5%.

[0025] Acquisition of initial buds

[0026] 2.1. Collection of explants

[0027] On the basis of the selection of excellent individual plants, from November to December of the previous year, the selected 4-terpineol type Melaleuca alternifolia excellent individual plants were felled or circumcised to promote germination (tree cutting 1 / 2 of the circumference), cut to the phloem. When the sprouts grow to 30-40cm, they are collected and inoculated in the afternoon on a sunny day.

[0028] 2.2. Disinfection of explants

[0029] After removing the leaves from the branches taken back, clean the soil and other pollutants attached to the surface o...

Embodiment 2

[0038] Selection of excellent individual plants

[0039] Select excellent individual plants with high biomass and high oil content in the Melaleuca alternifolia forest, whose essential oil index reaches the international standard IS04730-2004 for "tea tree oil", its 4-terpineol content> 35%, 1,8-eucalyptus Leaf element content <5%.

[0040] Acquisition of initial buds

[0041] 2.1. Collection of explants

[0042] On the basis of the selection of excellent individual plants, from November to December of the previous year, the selected 4-terpineol type Melaleuca alternifolia excellent individual plants were felled or circumcised to promote germination (tree cutting 2 / 3 of the circumference), cut to the phloem. When the sprouts grow to 30-40cm, they are collected and inoculated in the afternoon on a sunny day.

[0043] 2.2. Disinfection of explants

[0044]After removing the blades from the branches taken back, clean the dirt and other pollutants attached to the surface of...

Embodiment 3

[0053] Selection of excellent individual plants

[0054] Select excellent individual plants with high biomass and high oil content in the Melaleuca alternifolia forest, whose essential oil index reaches the international standard IS04730-2004 for "tea tree oil", its 4-terpineol content> 35%, 1,8-eucalyptus Leaf element content <5%.

[0055] Acquisition of initial buds

[0056] 2.1. Collection of explants

[0057] On the basis of the selection of excellent individual plants, from November to December of the previous year, the selected 4-terpineol type Melaleuca alternifolia excellent individual plants were cut down or circumcised to promote germination (circumcision) a full week), cut to the phloem. When the sprouts grow to 30-40cm, they are collected and inoculated in the afternoon on a sunny day.

[0058] 2.2. Disinfection of explants

[0059] After removing the blades from the branches taken back, clean the dirt and other pollutants attached to the surface of the bran...

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Abstract

The invention discloses a 4-terpilenol-type melaleuca alternifolia tissue culture subculture multiplication method. The method comprises the following steps: selecting a superior individual plant of 4-terpilenol-type melaleuca alternifolia to stump or cut annularly for promoting sprouting, collecting sprouting branches, and culturing through specially designed early-generation conductive culture medium and subculture multiplication culture medium in sequence. Experiments show that, by applying the method, the propagation coefficient of bud organs can be improved, the subculture multiplication period of the bud organs can be shortened and the number of effective buds can be increased, so that the production cost of tissue culture industrialized seedling of the 4-terpilenol-type melaleuca alternifolia is reduced; the superior individual plant of the 4-terpilenol-type melaleuca alternifolia is adopted as an explant, and a tissue culture seedling of the explant remains excellent genetic traits of a female parent, so that improved variety system and clone of the melaleuca alternifolia are achieved; the biomass and oil production per unit area can be increased, and the economic benefit and the ecological benefit of melaleuca alternifolia forest stand are improved.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and seedling cultivation of Melaleuca alternifolia, in particular to a 4-terpineol type Melaleuca alternifolia tissue culture subculture propagation method and a culture medium thereof. Background technique [0002] Melaleuca alternifolia Linn is a plant of the Myrtaceae Melateuca genus, commonly known as tea tree, evergreen shrub to small tree, native to Queensland and northern New South Wales in eastern Australia. Because the branches and leaves of Melaleuca alternifolia contain volatile components such as α-terpinene, eucalyptol, γ-terpinene, 4-terpineol, etc., it has been widely planted as a spice plant in Australia for a long time. The price of different biochemical types of Melaleuca alternifolia oil varies greatly, and the uses are also different; 4-terpineol type Melaleuca alternifolia oil, which is often called tea tree oil, has the highest value; eucalyptol type Melaleuca alternif...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 刘海龙覃子海陈博雯肖玉菲张烨
Owner GUANGXI FORESTRY RES INST
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