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A method for establishing Lycoris regeneration system

A Lycoris and system technology, applied in the field of plant tissue culture, can solve the problems of slow multiplication, difficulty in normal fruiting, and low multiplication coefficient, and achieve the effects of accelerating multiplication, improving genetic traits, and increasing multiplication coefficient

Active Publication Date: 2019-01-11
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the natural state, Chinese lycoris and other plants of the genus Lycoris are generally propagated by natural division of bulbs, but the reproduction coefficient is low. On average, one flowering bulb produces 1 to 2 bulbs every year, and it takes about 3 to 4 years for the bulbs to bloom. ; Even if it bears fruit normally, it generally takes 5-6 years from seed germination to flowering; and many species of Lycoris are not easy to bear fruit normally
Therefore, the speed of sphere propagation is slow and the propagation cycle is long, which is a difficult problem faced in the popularization and application of the whole Lycoris plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Take a healthy Chinese Lycoris underground bulb with a diameter of 2.0cm, peel off the outer scale leaves, cut off all the roots and 1 / 2 of the upper half of the bulb, save the bulb disc, brush the soil on the root surface, and cut according to the size of the bulb disc Into 4 pieces, each piece is 1.0cm in size 2 , 84 detergent shake and wash for 30min, rinse with running water for 30min, standby.

[0043] The above materials are rinsed with 75% alcohol and 0.1% HgCl on the ultra-clean workbench for 30s 2 Sterilize for 40 minutes, rinse with sterile water 4 times, and absorb the moisture on the surface with sterile absorbent paper.

[0044] The sterilized bulbs were directly inoculated onto the bud induction medium with the base plate down. The composition of the medium was: sucrose 30g / L+agar 7.5g / L+MS+3.0mg / L 6-BA +1.5mg / L NAA. After 28 days of culture, small buds grew from the scales of the explants. Subculture was carried out after 40 days. Culture temperature 25℃, l...

Embodiment 2

[0053] Take a healthy and healthy Chinese Lycoris underground bulb with a diameter of 3.0cm, peel off the outer scale leaves, cut off all the roots and 2 / 3 of the upper half of the bulb, save the bulb disc, and brush the soil on the root surface according to the size of the bulb disc Cut into 4 pieces, each piece is 1.0cm in size 2 , 84 detergent shake washing for 28min, running water rinse for 33min, standby.

[0054] The above materials are rinsed with 75% alcohol and 0.1% HgCl for 33s on the ultra-clean workbench 2 Sterilize for 42 minutes, rinse with sterile water 5 times, and absorb water on the surface with sterile absorbent paper.

[0055] The sterilized bulbs were directly inoculated onto the bud induction medium with the base plate down. The composition of the medium was: sucrose 30g / L+agar 7.5g / L+MS+3.0mg / L 6-BA +1.5mg / L NAA. After 28 days of culture, small buds grew from the scales of the explants. Subculture was carried out after 40 days. Culture temperature 26℃, lig...

Embodiment 3

[0064] Take a healthy and healthy Chinese Lycoris underground bulb with a diameter of 4.0cm, peel off the outer scale leaves, cut off all roots and 3 / 5 of the upper half of the bulb, save the bulb disc, and brush the soil on the root surface according to the size of the bulb disc Cut into pieces, each piece is 1.2cm in size 2 , 84 detergent shake and wash for 33 minutes, rinse with running water for 30 minutes, and spare.

[0065] The above materials are rinsed with 75% alcohol and 0.1% HgCl on the ultra-clean workbench for 28s 2 Sterilize for 35 minutes, rinse with sterile water 3 times, and absorb water on the surface with sterile absorbent paper.

[0066] The sterilized bulbs were directly inoculated onto the bud induction medium with the base plate down. The composition of the medium was: sucrose 30g / L+agar 7.5g / L+MS+3.0mg / L 6-BA +1.5mg / L NAA. After 28 days of culture, small buds grew from the scales of the explants. Subculture was carried out after 40 days. Culture temperat...

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Abstract

The invention provides a method for establishing a lycoris regeneration system. The method comprises the following steps: 1) carrying out sterilization, multiple shoot induction, adventitious bud proliferation and strong bud culture on underground bulbs of lycoris to obtain newborn bulbs; 2) inoculating the newborn bulbs to a callus inducing culture medium to be subjected to dark culture so as to obtain callus tissues of which the diameters are 1.5 to 2.5cm; 3) cutting the callus tissues into clumps of which the diameters are 1 to 2cm and then inoculating to a shoot induction culture medium to differentiate the callus tissues for 15 to 20 d to obtain multiple shoots; and 4) inoculating the multiple shoots to a strong bud rooting culture medium to be subjected to strong bud rooting culture so as to obtain tissue culture seedlings. The multiple shoots induced by the callus tissues are large in number, high in growth speed and capable of greatly improving the lycoris propagation coefficient and further increasing the propagation speed; and in addition, a high-efficient genetic transformation system of the lycoris is expected to be established through the callus tissues to realize genetic improvement on the molecular biology level of the lycoris.

Description

Technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for establishing a Lycoris radiata regeneration system. Background technique [0002] Lycoris chinensis Traub (Lycoris chinensis Traub) is a perennial herb of the Amaryllidaceae (Amaryllidaceae) Lycoris (Lycoris Herb.). The bulb is ovoid, leaves in spring, the leaves are ribbon-shaped, and the umbels have 5-6 small flowers; the flowers are yellow; the perianth lobes are intensified and shrunken. Flowering from July to August. The flower stems are tall and straight, the flower shape is beautiful, the flower color is gorgeous, and it has high ornamental value. It has gradually become a new type of fresh cut flowers, garden ground covers and potted bulbous flowers. [0003] Under natural conditions, Chinese Lycoris and other Lycoris plants generally reproduce naturally by dividing bulbs, but the reproduction coefficient is low. An flowering bulb produces 1 to 2 bulb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郑玉红张鹏翀佴辉鲍淳松樊聪束晓春韩福贵
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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