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Composite amplification kit for 26 Y chromosome short tandem repeats

A technology of compound amplification and compound amplification system is applied in the field of simultaneous amplification of multiple short tandem repeat loci and detection of genetic marker genes with polymorphism in the human genome. Application, waste of Y-DNA database data resources, etc., to achieve the effect of high individual recognition rate and high compatibility

Active Publication Date: 2014-09-03
BEIJING PEOPLESPOT TECH
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AI Technical Summary

Problems solved by technology

In this way, not all data are valid when comparing, but only the data of the same loci can be compared, while different parts cannot be applied
Therefore, due to the different loci of each Y-STR kit, some data resources of the Y-DNA database are wasted, and the effectiveness is not enough

Method used

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  • Composite amplification kit for 26 Y chromosome short tandem repeats
  • Composite amplification kit for 26 Y chromosome short tandem repeats
  • Composite amplification kit for 26 Y chromosome short tandem repeats

Examples

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Embodiment 1

[0044] Example 1 Compound amplification of 26 gene loci and analysis of their genotypes

[0045] Purchase 9948 cell line. Template DNA was extracted from the cell line using the chelex-100 method. The amplification reaction was carried out on the ABI 9700 thermal cycler, the electrophoresis and detection were carried out on the ABI 3100 genetic analyzer, and the data analysis was performed using GeneMapper ID v3.2 software. The reagents and materials used in the present invention, such as allelic ladder (ladder), are conventional materials commonly used by those skilled in the art.

[0046] 1.1. chelex-100 method to extract DNA (specific method refer to "Forensic DNA Protocol", Humana Press, 1998)

[0047] 1) Take 3 μl of cell lines in a 500 μl centrifuge tube

[0048] 2) Shake and mix the chelex solution to make the chelex fully suspended, add 195 μl of 5% chelex-100 (100-200 mesh, purchased from Bio-Rad) to each tube, and then add 5 μl proteinase K (20 mg / ml, purchased fr...

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Abstract

The invention relates to a composite amplification system for simultaneously analyzing multiple Y-STR loci. The composite amplification kit is characterized by compositely amplifying the following 26 Y chromosome loci: DYS19, DYS388, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a, DYS385b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, DYS460, DYS481, DYS533, DYS549, DYS570, DYS576, DYS643, DYS449 and Y-GATA-H4. The invention also relates to a method and a kit for simultaneously analyzing DNA samples, and applications thereof.

Description

Technical field: [0001] The present invention relates to the field of biotechnology. Further, the present invention relates to the detection of polymorphic genetic marker genes in the human genome. In particular, the present invention relates to the simultaneous amplification of multiple short tandem repeat loci in one amplification system using polymerase chain reaction. Background technique: [0002] Short tandem repeats (STR for short), also known as microsatellites or simple sequence repeats (SSR), are a type of DNA tandem repeats widely present in eukaryotic genomes, with a core sequence of 2-6 base repeat units. The number of STR loci is large and widely distributed, accounting for about 3% of the entire genome (International Human Genome Sequencing Consortium, 2001), and the polymorphism is high, and its polymorphism mainly comes from the difference in the number of core sequence repeats between individuals, and This difference follows the Mendelian law of inheritan...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 张威张琰
Owner BEIJING PEOPLESPOT TECH
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