Multiplex Amplification Kit for 20 Y Chromosome Short Tandem Repeats
A composite amplification, primer sequence technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of insufficient effectiveness, inability to use different parts, waste of DNA database data resources, etc. Achieve the effect of high compatibility and high individual recognition rate
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[0047] Example 1 multiplex amplification of 20 Y-STR gene loci and analysis of their genotypes
[0048] Purchase 9948 cell line. Template DNA was extracted from the cell line using the chelex-100 method. The amplification reaction was carried out on the ABI9700 thermal cycler, the electrophoresis and detection were carried out on the ABI3100 genetic analyzer, and the data analysis was performed using GeneMapper ID v3.2 software. The samples were tested with AmpFISTR Yfiler kit from ABI company and AGCU Y18 kit from Zhongde Meilian company. The operation method was carried out according to the kit instructions, and the results were used as a control. The reagents and materials used in the present invention, such as allelic ladder (ladder), are conventional materials commonly used by those skilled in the art.
[0049] 1.1. chelex-100 method to extract DNA (specific method refer to "Forensic DNA Protocol", HumanaPress, 1998)
[0050] 1) Take 3 μl of cell lines in a 500 μl cent...
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