Complex aplification detecting system of fluorescent marker short tandem repetitive sequence gene locus

A compound amplification system and compound amplification technology are applied in the field of fluorescently labeled STR compound amplification inspection systems, and achieve the effects of high sensitivity, accurate typing results and good balance.

Inactive Publication Date: 2006-01-25
公安部第二研究所
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  • Complex aplification detecting system of fluorescent marker short tandem repetitive sequence gene locus
  • Complex aplification detecting system of fluorescent marker short tandem repetitive sequence gene locus
  • Complex aplification detecting system of fluorescent marker short tandem repetitive sequence gene locus

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Embodiment Construction

[0021] 1. Determination of genetic loci

[0022] Perform in-depth analysis of existing STR loci and optimize new high-discrimination loci. 对D8S1179、D21S11、D7S820、CSF1P0、D3S1358、D13S317、D16S539、TPOX、TH01、D5S818、vWA、D18S51、FGA、D2S1338、D19S433、Penta E、Penta D、D19S253、D12S391、D1S1612、D9S302、D18S535、D15S816、D6S1043 and other 24 STR loci in the Chinese population genetic polymorphism investigation. The study established the STR locus composition, namely D2S1338, D6S1043, D21S11, D7S820, CSF1PO, D3S1358, D8S1179, D16S539, PentaE, D5S818, vWA, D13S317, D18S51 and FGA. The composition of this gene locus breaks through the existing pattern of 13 core loci (recommended by the FBI), and is more in line with the technical requirements of forensic DNA testing and the population genetics characteristics of the Chinese population.

[0023] 2. Design of locus combination scheme for fluorescently labeled STR multiplex amplification system

[0024] In this study, fluorescent dyes were identifi...

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Abstract

The invention supplies a fluorescence labeling verification system by compounding and expanding 14 short series repeating sequence locus to take personal identification and paternity test. The specific compounding method controls the expanding product in the range of 400 basic groups, and only labeling three fluorescence of the compounding expanding of the 14 locus would be realized. The invention takes a deep research to the gene characteristic of the 14 locus and the allele in five minorities in China, and supplies the compounding expanding verification system. The agent box suited for Chinese throng is researched.

Description

technical field [0001] The present invention relates to the detection of polymorphic genetic markers in the human genome. In particular, the invention relates to the simultaneous amplification of multiple short tandem repeat loci in one reaction using the polymerase chain reaction. The invention also relates to a fluorescence-marked STR compound amplification inspection system for personal identification and paternity identification through compound amplification of 14 STR loci. The inspection system of the present invention is suitable for Chinese population. Background technique [0002] Short tandem repeat loci (STR) are currently commonly used genetic markers. In the early 1990s, the polymorphism of the STR locus was discovered, especially the STR locus has a small fragment and is easy to amplify, which is suitable for testing trace and degraded samples, and the amplification conditions of each locus are similar and can be compounded and amplified, so it has the advant...

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 叶健季安全姜成涛
Owner 公安部第二研究所
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