Composite amplification system of 23 short tandem repeat sequences and a kit

A compound amplification system and short tandem repeat technology, applied in the biological field, to achieve high system efficiency and good independence

Active Publication Date: 2014-06-04
SUZHOU MICROREAD GENETICS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Most of the kits currently used are 13 CODIS (Combined DNA Index System) selected by the US FBI: CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11 are Basic CODIS locus kit, but in some assays, more non-CODIS loci are needed to provide more information

Method used

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  • Composite amplification system of 23 short tandem repeat sequences and a kit
  • Composite amplification system of 23 short tandem repeat sequences and a kit
  • Composite amplification system of 23 short tandem repeat sequences and a kit

Examples

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Embodiment 2

[0121] Example 2 Application of the compound amplification kit of 23 short tandem repeats for paternity identification

[0122] 1. Collect blood samples for paternity testing (blood samples are provided by a genetic testing center)

[0123] 2. Use the Chelex-100 method to extract genomic DNA (refer to "Forensic DNA Protocol". Humana Press, 1998). Take 0.5-5 μl of anticoagulated whole blood or 1-3mm×2-5mm blood spots in a 500μl centrifuge tube, shake Mix Chelex solution to make Chelex fully suspended, add 195μl Chelex-100 (5%) solution and 5μl proteinase K (20mg / ml) to each tube, shake and mix well, keep warm at 56°C for two hours or overnight, take it out and shake for 2 minutes, put in boiling water After heating for 10 minutes, centrifuge at 13,000 rpm for 5 minutes, and carefully pipette 150 μl of the supernatant into a new centrifuge tube.

[0124] 3. Perform PCR amplification according to the amplification conditions in Example 1.

[0125] 4. Detect on the genetic analy...

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Abstract

The invention relates to a composite amplification system of 23 short tandem repeat sequences and a kit, is used for detecting heredity mark gene of polymorphism in a mankind genome, and belongs to the biology technical field. The invention relates to a scheme that a plurality of short tandem repeat sequences are simultaneously amplified in a PCR system; a specific gene locus comprises 22 short tandem repeat sequences with high level heredity polymorphism and 1 gender determination locus; primers are respectively designed and a fluorophore is marked; the system has very high individual recognition rate and non-father elimination rate; the system and kit can be used for legal medical individual recognition, paternity tests, and colony genetics analysis, is high in accuracy, and good in sensitivity.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a compound amplification system for identifying multiple human short tandem repeat sequences, which can rapidly and accurately perform human STR typing and provide a basis for forensic individual identification and paternity identification. Background technique [0002] Human genome STR (Short Tandem Repeats, short tandem repeat sequence) is a relatively stable sequence in DNA inheritance formed by tandem repetition of several bases as the core unit. The distinction between different races, different populations and different individuals is through the difference in core unit sequence and repeat number, which also constitutes the genetic polymorphism of STR. In the genome, there is an STR site every 15-20kb on average, accounting for 10% of the genome, mostly in non-coding regions and introns, the repeat unit is 2-6bp, the number of repeats is 10-60 times, and the fragment size In 70 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 陈初光于在亮宋欣欣付亮剑
Owner SUZHOU MICROREAD GENETICS
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