SNP (Single Nucleotide Polymorphism) molecular marker for identifying age-round shearing amount of alpine merino and application of SNP molecular marker

A Merino sheep and molecular marker technology, applied in the field of molecular genetics, can solve the problems that important genetic resources have not been effectively excavated and utilized, and the molecular marker system for fine-haired wool traits has not been established, so that the results can be easily interpreted and improved. The effect of high breeding accuracy and technical accuracy

Active Publication Date: 2022-06-24
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, fine-wool sheep genetics and breeding experts at home and abroad have been devoting themselves to the research of candidate genes or molecular markers controlling wool traits, but no molecula

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker for identifying age-round shearing amount of alpine merino and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker for identifying age-round shearing amount of alpine merino and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker for identifying age-round shearing amount of alpine merino and application of SNP molecular marker

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Embodiment 1

[0037] Embodiment 1 Correlation between different genotypes of Alpine Merino sheep and annual shearing amount

[0038] 1. Sample Collection

[0039] The Gansu Provincial Sheep Breeding Technology Promotion Station collected blood samples from 109 Alpine Merino sheep. 5 mL of blood was collected intravenously from each sheep in a blood collection tube with EDTA-K2 anticoagulant added. Temporarily stored in the box, transported back to the laboratory and stored in -20°C freezer for DNA extraction. The annual shearing quantity record of each sheep was provided by Gansu Provincial Sheep Breeding Technology Extension Station.

[0040] 2. Main reagents and instruments

[0041] EDTA-K2 vacuum blood collection tube was purchased from Jiangsu Yuli Medical Instrument Co., Ltd.; blood genome extraction kit was purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.; NanoDrop2000 Spectrophotometer Thermo Fisher Scientific Company of America; DL2000 Marker, agarose, nucleic aci...

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Abstract

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker for identifying the age-round shearing amount of alpine merino and application of the SNP molecular marker. The SNP molecular marker is located at the 20077683th nucleotide site G/C mutation on the fifth chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the annual shearing amount of the alpine merino sheep, shortening the breeding period and accelerating the breeding process, an early selection technology of the annual shearing amount of the alpine merino sheep is established, the breeding time of the excellent character of the annual shearing amount of the alpine merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

Description

technical field [0001] The invention belongs to the technical field of molecular genetics, and in particular relates to a SNP molecular marker for identifying the annual shearing amount of Alpine Merino sheep and its application. Background technique [0002] Wool refers to a layer of textile-worthy fibers covering the surface of the sheep's body and is a derivative of the skin. Wool is the main raw material of wool spinning industry, accounting for about 97% of wool spinning raw materials, and is mainly used to process clothing fabrics, wool, blankets and other products. In wool production and breeding practice, wool traits are important economic traits, mainly including hair length, wool yield, average fiber diameter, bending, breaking strength, elongation, net wool rate, shearing amount and other indicators. Weaving products and economic benefits are closely related. Due to population growth, demand for wool has exceeded supply. As market demands change, producing high...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125Y02P60/87
Inventor 卢曾奎袁超郭婷婷杨博辉刘建斌岳耀敬李建烨牛春娥
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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