Chloride ion passage gene fragment related to pig intramuscularly fat deposition and use thereof

Abstract

The invention discloses a chloride channel gene fragment related to intramuscular fat deposition of pigs and application thereof. The DNA fragment related to intramuscular fat deposition of pigs is shown in Sequence I of a Sequence Table. The invention also provides a method of applying the DNA fragment to detect the intramuscular fat deposition of pigs in an aided manner. The DNA fragment of the invention has a very obvious connection with the intramuscular fat deposition of pigs. Compared with the traditional methods, through the detection of pigs at birth by the method of the invention, the intramuscular fat deposition of pigs can be known at the earliest possible time, in addition, early selection can be realized and the breeding selection can be shortened, thus greatly reducing the expenses of the breeding selection. Meanwhile, in the method of the invention, a functional DNA fragment with effects is directly selected, thus improving the accuracy of the breeding selection and reducing the waste of manpower and materials. The invention has very great economic value and social significance.

Description

technical field [0001] The invention relates to a chloride ion channel gene fragment related to pig intramuscular fat deposition and application thereof. Background technique [0002] Intramuscular fat refers to the fat deposited between muscle fibers or inside muscle fibers. It is closely related to meat quality and is one of the key factors affecting meat quality. The increase of intramuscular fat deposition can significantly improve the juiciness, color, flavor, tenderness and water holding capacity of meat. It is generally believed that the meat quality of pork with intramuscular fat deposition of 3.0% is better. [0003] Because the molecular mechanisms underlying intramuscular fat deposition are poorly understood, there are no effective genetic markers (or molecular markers) for this trait. In view of the genetic complexity of the trait of intramuscular fat deposition, it is currently impossible to effectively select for this trait in breeding. We can only use local p...

AI Technical Summary

Problems solved by technology

Although the B-ultrasound method has greatly improved compared with the ultrasonic method, due to the lack of understanding of the genetic law of intramuscular fat deposition, the effect in breeding has been unsatisfactory and the expected goal has not been achieved.

On the other hand, the degree of selective breeding of local pig breeds in my country is relatively low, and the same pig breed also contains rich genetic diversity. The effect of quality is uneven, and the genetic potential of excellent meat quality of local pig breeds in my country cannot be stably and effectively exerted

Due to the lack of effective breeding methods, it is difficult to achieve a breakthrough in intramuscular fat deposition by hybrid breeding methods, and it is also difficult to maintain and fix the hybrid advantages of local pig breeds and lean pigs in subsequent subbreeding

In a word, as far as the current breeding and breeding methods are concerned, increasing the amount of fat deposition in the muscle means increasing the deposition of subcutaneous fat and reducing the lean meat rate. Therefore, how to increase the amount of fat deposition in the muscle while maintaining less subcutaneous fat, It is a difficult problem facing meat quality regulation

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