23 results about "Chloride channel" patented technology

The invention belongs to the technical field of molecular markers, and particularly relates to application of a chloride channel protein coding gene GmSALT9 in identification of soybean salt tolerance. The nucleotide sequence of the chloride ion channel protein coding gene GmSALT9 disclosed by the invention is as shown in SEQ ID NO. 1. According to the scheme, the salt tolerance of the soybean can be judged according to the promoter haplotype of the chloride ion channel protein encoding gene GmSALT9; when the promoter haplotype of the GmSALT9 is a TT type, it is prompted that the salt tolerance of the tested soybeans is high; when the promoter haplotype of the GmSALT9 is an AA type, it is prompted that the detected soybean is sensitive to salt. The invention provides remarkable relevance between the chloride ion channel protein coding gene GmSALT9 and the salt-tolerant character of the soybean, and the chloride ion channel protein coding gene GmSALT9 has important research value and application prospect in improvement of the salt-tolerant character of the soybean and cultivation of a new salt-tolerant soybean variety.

The present invention relates to an insecticidal and acaricidal composition. The composition contains active component A and active component B, wherein the active component A is selected from compound I; the active component B is selected from one or more of bionic juvenile hormones, chitin biosynthesis inhibitors affecting CHS1, type-1 chitin biosynthesis inhibitors, ecdysone receptor agonists, nicotinic acetylcholine receptor competitive modulators, ryanodine receptor modulators, nicotinic acetylcholine receptor allosteric modulators, glutamate-gated chloride channel allosteric modulators, sodium channel modulators, voltage-dependent sodium channel blockers, inhibitors, trifluoroethyl sulfide acaricides, other insecticides and acaricides, etc.; and the active component A has a structure as follows. The composition of the present invention has advantages such as obvious synergistic effects and delayed resistance development, and can be used for controlling a variety of pests.

Engineered glutamate-gated chloride channels (GluCl) useful in the treatment of disease are disclosed herein. Chimeric fusion proteins are described, alongside nucleic acids, expression vectors, recombinant virus particles and engineered cells configured to encode or express the chimeric fusion proteins and / or engineered GluCls. Corresponding methods of treatment and uses of the engineered GluCls, chimeric fusion proteins, nucleic acids, expression vectors, recombinant virus particles and engineered cells are also provided.

The application discloses a method for precisely regulating wax synthesis of plant leaves based on light-controlled chloride ion channel protein and belongs to the technical field of genetic engineering. The specific process is as follows: the light-controlled chloride ion channel protein opens or closes the ion channel under irradiation of different light, and the extracellular flow of chloride ions is promoted or terminated, so that the effect of promoting or terminating the wax synthesis is achieved. According to the principle of optogenetics, the application realizes the precise, reversible and space-time controllable regulation of the wax synthesis of the plant leaves, and assists in mining more genes related to the wax synthesis of the plants, thereby providing technical support for improving the adaptation ability of the plants to adversity.

The present invention discloses a γ-aminobutyric acid-gated chloride channel allosteric modulator compound and its use, namely (S)-4-(5-(3-chloro-5-(trifluoromethyl)phenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-yl)-2-methyl-N-(2-oxo-2-((2,2,2-trifluoroethyl)amino)ethyl)benzamide. The compound has excellent control effects on pests such as Thysanoptera, Acarina, and Lepidoptera, is very friendly to bees, has high environmental and ecological safety, can be used to prepare high-efficiency, low-toxic, and safe insecticides, and has broad application prospects in agricultural production.

This invention belongs to the field of pesticide technology, specifically relating to an insecticidal composition containing cybenzoxasulfyl and its application. The insecticidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is cybenzoxasulfyl; active ingredient B is selected from one or more of the following compounds: acetylcholinesterase inhibitors, nicotinic acetylcholine receptor (nAChR) channel blockers, nicotinic acetylcholine receptor (nAChR) allosteric modulator site I, nicotinic acetylcholine receptor (nAChR) competitive modulators, glutamate-gated chloride channel (GluCl) allosteric modulators, etc. This composition has a reasonable composition, good insecticidal effect, low application cost, and features reduced application amount, crop safety, and reduced pesticide residues.

The present disclosure relates to methods of treating bile acid diarrhea by administering a CFTR chloride channel inhibitor (CFTR-CCI). In some embodiments, the CFTR chloride channel inhibitor may be a benzopyrimidino-pyrrolo-oxazine-dione-CFTR-CCI (e.g., BPO-27), a PPQ-CFTR-CCI, a thiazolidinone-CFTR-CCI, or a glycine hydrazide-CFTR-CCI, or a pharmaceutically acceptable salt thereof.

Disclosed in the present invention are a γ-aminobutyric acid gated chloride ion channel allosteric modulator compound and the use thereof, i.e., (S)-4-(5-(3-chloro-5-(trifluoromethyl)phenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazole-3-yl)-2-methyl-N-(2-oxo-2-((2,2,2-trifluoroethyl)amino)ethyl)benzamide. The compound has an excellent control effect on pests such as thysanoptera, acarina and lepidoptera, is very friendly to bees, has high environmental ecological safety, can be used for preparing efficient, low-toxicity and safe insecticides, and has wide application prospects in agricultural production.

The invention belongs to the technical field of pesticide compounding, and particularly relates to a composition for preventing and treating pine wood nematodes, a preparation and application of the composition and the preparation. Effective active components in the composition comprise an active component A and an active component B, the concentration interaction ratio of the active component A to the active component B is 1: (0.1-10), the active component A is abamectin or emamectin benzoate, and the active component B is rotenone. Plant source active components and chemical pesticide components are compounded to improve the prevention and control effect, the pesticide dosage is reduced, the environmental risk is reduced, a binary compound system of rotenone and abamectin chemical agents is innovatively constructed, and the pesticide composition has a good application prospect in prevention and control of rotenone and abamectin chemical agents. Through cooperation of different action mechanisms of inhibition of mitochondrial respiratory chains of the two and blocking of glutamic acid gated chloride ion channels, multi-target, low-risk and high-efficiency prevention and control targets are achieved, and a green and efficient compound pesticide is provided for green and sustainable prevention and control of pine wood nematode diseases.

An insecticidal composition containing cybenzoxasulfyl and the use thereof. The insecticidal composition contains active ingredient A and active ingredient B, wherein active ingredient A is cybenzoxasulfyl, and active ingredient B is selected from one or more of the following compounds: an acetylcholinesterase inhibitor, a nicotinic acetylcholine receptor (nAChR) channel blocker, nicotinic acetylcholine receptor (nAChR) allosteric modulator site I, a nicotinic acetylcholine receptor (nAChR) competitive modulator, a glutamate-gated chloride channel (GluCl) allosteric modulator, etc. The composition has reasonable components, good insecticidal effects, and low administration costs, and also has the characteristics of a reduced application amount, safety to crops, reduced pesticide residues, etc.

The invention belongs to the field of insecticides and acaricides, and relates to a harmful arthropod prevention and treatment composition containing alanine-isoxazoline compounds. The composition contains an active component A and an active component B, and the weight part ratio of the active component A to the active component B is (1: 99)-(99: 1); the active component A is selected from a compound I, a compound II or a stereoisomer thereof, and the active component B is selected from a nicotinic acetylcholine receptor (nAChR) allosteric modulator or a glutamic acid gated chloride ion channel (GluCl) allosteric modulator; the composition disclosed by the invention has the advantages of obvious synergism, resistance delaying and the like, and can be used for preventing and treating various pests.

An insecticidal and acaricidal composition of the present invention comprises an active component A and an active component B, the ratio of weight parts of the active component A to the active component B being 1:100-100:1. The active component B is selected from one or more of bionic juvenile hormones, chitin biosynthesis inhibitors that affect CHS1, type-1 chitin biosynthesis inhibitors, ecdysone receptor agonists, nicotinic acetylcholine receptor competitive modulators, ryanodine receptor modulators, nicotinic acetylcholine receptor allosteric modulators, glutamate-gated chloride channel allosteric modulators, sodium channel modulators, voltage-dependent sodium channel blockers, inhibitors, trifluoroethyl sulfide acaricides and other insecticides and acaricides. The active component A is selected from a compound (I), a compound (II) having the following structures, or stereoisomers thereof. The insecticidal and acaricidal composition of the present invention has the advantages such as obvious synergistic effect and delayed development of drug resistance, and can be used for controlling a variety of pests.

The application belongs to the field of biological medicine, and relates to application of a CLIC1 inhibitor in preparation of an anti-zika virus infection drug, wherein the CLIC1 inhibitor comprises at least one of the following two substances: I: a substance for inhibiting expression of a CLIC1 gene in a host cell; II: a substance for inhibiting a CLIC1 chloride channel function in the host cell. The application first discloses that the CLIC1 gene and protein are key host factors for zika virus replication, and zika virus replication can be blocked by inhibiting the expression or chloride channel function, thereby providing a new direction for development of an anti-zika virus drug targeting a host. Compared with traditional drugs targeting zika virus proteins, the anti-zika virus infection drug prepared by using the CLIC1 inhibitor targets a host factor, is more difficult to induce virus drug resistance, and has more persistent treatment potential.

The present application relates to the field of biotechnology, and particularly relates to a method for identifying mutation of a gene of glutamate-gated chloride channel of plutella xylostella and primers. The present application provides a protein mutant related to resistance to avermectin insecticide, wherein a valine at position 263 of a glutamate-gated chloride channel protein is mutated into isoleucine. The present application can identify the resistance of insects to avermectin insecticide.

This invention relates to the biomedical field and discloses the application of the Ano5 gene as a therapeutic target for osteoporosis. This invention utilizes a previously constructed... Year 5 Gene knockout mouse model, construction Year 5 ‑ / ‑ The +OVX mouse model was used to clearly demonstrate the effects of inhibiting Ano5 on osteoporosis at the imaging, histological, biomechanical, serological, and molecular levels. Further research is needed on the inhibition... Year 5 The effects and molecular mechanisms of osteoporosis development, and using osteoclast ferroptosis as a starting point, reveal... Year 5 The mechanism by which gene defects inhibit bone resorption by promoting ferroptosis provides a theoretical basis for treating osteoporosis by targeting the chloride channel protein ANO5. This will contribute to the development of new targets for chloride channels in the treatment of osteoporosis.

The present invention belongs to the technical field of malignant glioma, and particularly relates to a targeted drug, preparation method and application for treating malignant glioma. The present invention prepares a chemical cross-linked complex of CTX and CLM, which has the potential to treat malignant glioma in a targeted manner. CTX acts as a targeted warhead to guide the complex and bind to the chloride ion channel specifically expressed by malignant glioma, and then causes the complex to enter the cell through endocytosis, thereby introducing CLM into the cell to kill tumor cells.

The invention relates to application of a glutamate transporter agonist in preparation of an ivermectin drug-resistant drug for treating parasites, and belongs to the field of drug resistance. The glutamic acid transporter agonist reduces the content of glutamic acid in the body by enhancing the function of the glutamic acid transporter. The ceftriaxone sodium is used for treating the ubr-1 mutant, the content of glutamic acid in the ubr-1 mutant is reduced, expression of a chloride ion channel controlled by glutamic acid is increased, and therefore IVM drug resistance caused by UBR-1 protein mutation is relieved. The invention finds that the curative effect of the IVM on parasitic diseases is effectively recovered, the service life of the medicine is prolonged, and the use cost of the IVM is reduced.

An insecticidal composition containing oxazosulfyl and a use thereof, relating to the technical field of pesticides. The insecticidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is oxazosulfyl; and the active ingredient B is selected from one or more of the following compounds: an acetylcholinesterase inhibitor, a nicotinic acetylcholine receptor (nAChR) channel blocker, an nAChR allosteric modulator site I, an nAChR competitive modulator, a glutamate-gated chloride channel (GluCl) allosteric modulator, etc. The composition has a reasonable formulation, good insecticidal efficacy, and low application costs, and has the characteristics of reduced application amount, safety to crops, reduced pesticide residue, etc.

The invention belongs to the field of biological medicine, and relates to an application of a CLIC1 inhibitor in preparation of Zika virus infection resistant drugs, the CLIC1 inhibitor comprises at least one of the following two substances: I: a substance for inhibiting CLIC1 gene expression in host cells; and II: a substance for inhibiting the function of a CLIC1 chloride ion channel in a host cell. The invention reveals that the CLIC1 gene and the protein are key host factors for replication of the Zika virus for the first time, the replication of the Zika virus can be blocked by inhibiting the expression of the CLIC1 gene or a chloride channel function, and a new direction is provided for developing the anti-Zika virus drug of a targeted host. Compared with a traditional Zika virus protein targeting drug which easily generates drug resistance due to virus mutation, the anti-Zika virus infection drug targeting host factor prepared by the CLIC1 inhibitor is more difficult to induce virus drug resistance and has more lasting treatment potential.

The present invention relates to pesticide mixtures comprising as active compound I a carboxamide derivative active as an insecticide and at least one active compound II selected from group M comprising acetylcholine esterase inhibitors, GABA-regulated chloride channel antagonists, sodium channel modulators, nicotinic acetylcholine receptor agonists / antagonists, allosteric nicotinic acetylcholine receptor activators, chloride channel activators, juvenile hormone mimics, homopteran feeding blockers, mite growth inhibitors, mitochondrial ATP synthase inhibitors, oxidative phosphorylation uncouplers, chitin biosynthesis inhibitors, molting disruptors, ecdyson receptor agonists, octamine receptor agonists, MET inhibitors, voltage-gated sodium channel blockers, lipid synthesis inhibitors,ryanodine receptor modulators and other compounds defined in the description, in synergistically effective amounts. The invention also relates to methods and the use of these mixtures to combat and control insects, arachnids, or nematodes in plants, and to protect these plants against pest infestation, in particular also to protect plant propagation material, such as seeds.

This invention belongs to the field of pesticide technology, specifically relating to an insecticidal composition containing oxazolidinone and its application. The insecticidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is oxazolidinone; active ingredient B is selected from one or more of the following compounds: acetylcholinesterase inhibitors, nicotinic acetylcholine receptor (nAChR) channel blockers, nicotinic acetylcholine receptor (nAChR) allosteric modulator site I, nicotinic acetylcholine receptor (nAChR) competitive modulators, glutamate-gated chloride channel (GluCl) allosteric modulators, etc. This composition has a reasonable composition, good insecticidal effect, low application cost, and features reduced application amount, crop safety, and reduced pesticide residues.

The present invention relates to a gRNA for constructing an osteopetrosis mouse model, a construction method and an application thereof. The nucleotide sequence of the gRNA is shown as SEQ ID NO: 1. The human CLCN7 (NM_001287.5: c.856C>T) mutation involved in the present invention can cause changes in the function of chloride channels, abnormal development of bone tissue, and thus lead to the occurrence of osteopetrosis. By designing a gRNA sequence specifically targeting CLCN7 (R286W) and using the CRISPR / Cas9 gene editing technology, the present invention successfully constructs the first homozygous mouse model of Clcn7 (r284w) carrying a mutation corresponding to human CLCN7 (R286W), filling the research gap in the related field.