31results about How to "Improving Breeding Accuracy" patented technology

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker influencing the wool shearing amount of alpine merino and application. The SNP molecular marker is located at the 20065540th nucleotide site G / A mutation on the fifth chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the shearing amount of the high-mountain merino sheep, shortening the breeding period and accelerating the breeding process, a high-mountain merino sheep shearing amount early selection technology is established, the breeding time of the excellent character of the shearing amount of the high-mountain merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

The invention provides an SNP (single nucleotide polymorphism) molecular marker combination for identifying the genetic relationship of Chinese Simmental. The marker combination contains 50 SNP markers distributed on 25 autosomes, wherein the average distance between adjacent SNP markers on the same autosome is 27M. The average values of the minor allele frequency (MAF), expected heterozygosity (HExp) and polymorphism information content (PIC) of the marker combination are 0.4818, 0.4998 and 0.3748 respectively; and when the genotype of the female parent is unknown, the accumulative exclusion probability is 0.9989. The SNP marker combination provided by the invention can be applied to complete and accurate pedigree identification of the Chinese Simmental, can improve the breeding accuracy of the Chinese Simmental and accelerate the genetic progress, and has good application prospect and economic benefit.

The invention relates to molecular genetics, and provides genetic markers for establishing and identifying a dairy cattle molecule system spectrum and application of the genetic markers. The genetic markers consist of 8 loci which are respectively TGLA227, TLA122, BMC1207, BM103, INRA037, INRA134, BP7 and MB026. The invention further provides primers of the 8 microsatellite loci, a method for obtaining fluorescence labeling primers by using the primers to perform fluorescence labeling capillary electrophoresis, a method for performing genotype judgment by using Gene Mapper V3.0 software together with Gene Marker software V1.75, a method for performing genetic relationship identification by using Cervus3.0 software, and a method for establishing an application kit. The genetic markers can be applied to dairy cattle system spectrum identification and establishment, are capable of making important basis for increasing the accuracy of dairy cattle species selection and breeding, are capable of promoting the breeding progress of dairy cattle, and have good application prospects and economical benefits.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP molecular marker influencing weaning weight character of merinos and application. The SNP molecular marker is located at the 3566348 nucleotide site T / C mutation on the 23<rd> chromosome of the international sheep reference genome Oar_v4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair, and a nucleotide polymorphism detection method. Compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the weaning weight characters of high-mountain merinos, shortening the breeding period and accelerating the breeding process, an early selection technology of the weaning weight characters of the high-mountain merinos is established, the breeding time of the excellent weaning weight characters of the high-mountain merinos is shortened, the breeding cost is reduced, and the application value is very high.

The invention discloses a compound PCR amplification system and a detection kit for genotyping of the STR marker detection system for paternity identification and individual identification of yaks. The primers have fluorescent markers and can amplify 14 STR marker sites of yaks. These markers are highly polymorphic, unlinked among the markers, and have appropriate fragment sizes; the STR detection system obtains the genotyping results of 14 STR loci through the amplification and electrophoresis detection of the 14 pairs of primers; The paternity test and individual identification of yaks were carried out based on the type results and the gene frequency of each STR in yaks. The scheme of the present invention can effectively perform paternity identification and individual identification of yaks by comprehensively analyzing the typing results of STR loci, thereby improving the accuracy of pedigree in yak breeding work, speeding up the breeding process, and has good application prospects and economic value.

The invention belongs to the technical field of molecular genetics, and in particular relates to a SNP molecular marker affecting the weaning weight traits of Alpine Merino sheep and its application. The SNP molecular marker is located at the T / C mutation at the 3566348th nucleotide site on chromosome 23 of the International Sheep Reference Genome Oar_v4.0 version. The present invention also relates to a specific primer pair for detecting the above-mentioned SNP molecular marker using PCR technology, a kit containing the primer pair and a detection method for nucleotide polymorphism. Compared with the traditional detection method, the technology has high accuracy and is It has the advantages of fast speed, low cost, and easy judgment of results. Using SNP locus detection for early selection of weaning heavy traits in alpine Merino sheep, shortening the breeding cycle and speeding up the breeding process, establishing an early selection technology for weaning heavy traits in alpine Merino sheep, reducing the weaning weight of alpine Merino sheep The breeding time of excellent traits reduces the breeding cost and has high application value.

The invention relates to a cultivating method of hyriopsis cumingii with a golden yellow shell. The method is characterized by comprising the following steps of (1), selecting 4-6 parent hyriopsis cumingii; (2) taking the margin tissues of the pallium of the parent hyriopsis cumingii, extracting the total RNA (ribonucleic acid) and synthetic single stranded c RNA, performing SRAP (sequence-related amplified polymorphism) amplification by adopting primer combination, and selecting male and female individuals with specific strip sequences with the golden yellow shells after the amplification products are subjected to electrophoresis; (3) using the selected male and female parent hyriopsis cumingii to build a family and cultivating in a water flowing pool; (4) periodically examining the pregnant condition of the female hyriopsis cumingii, and if mature glochidiums are found, selecting the mature female hyriopsis cumingii to reproduce to obtain infant hyriopsis cumingii; cultivating the infant hyriopsis cumingii in the water flowing pool until the shell reaches 1-2cm long, removing into to a net cage, and suspending the net cage into a pond to perform second stage cultivating on the infant hyriopsis cumingii until the shell length reaches 5-8cm; (5) removing the family parent hyriopsis cumingii with the non-golden yellow shells, and using the rest parent hyriopsis cumingii to build a core cultivating population with the golden yellow shells. According to the cultivation method of the hyriopsis cumingii with the golden yellow shell, the ratio of the hyriopsis cumingii with the golden yellow shell can reach more than 99.0%, the shell color is stable in heredity and the postoperative survival rate is high.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker for identifying the weaning weight of alpine merino and application of the SNP marker, and the SNP molecular marker is located at the 3566034th nucleotide site C / T mutation on the 23rd chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the weaning weight of the high-mountain merino sheep, shortening the breeding period and accelerating the breeding process, a technology for early selection of the weaning weight of the high-mountain merino sheep is established, the breeding time of excellent characters of the weaning weight of the high-mountain merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

The invention belongs to the field of poultry molecular breeding, and relates to a duck egg shell color molecular marker and its application in duck egg shell color prediction and egg-type breeding duck selection. The nucleotide sequence of the molecular marker of the present invention is as shown in SEQ ID NO.1 or SEQ ID NO.2, and the change of the genotype of the above-mentioned molecular marker G1157A site can be analyzed by PCR and sequencing methods, which can be accurate, fast and effective The eggshell color prediction of duck eggs provides a powerful molecular biological basis and implementation method for duck breeding.

The invention relates to a SNP marker of blood-related infiltration sites of western pigs related to body length in Chinese native pigs and its application. The marker is at least one of the following mutations: International Porcine Genome Version 11.1 Reference Sequence at base 2096738 (rs81439116) of pig chromosome 12, a point mutation from A to G, at base 2129234 (rs81439242) , a point mutation from C to T, base 2145858 (rs81439307), a point mutation from T to C. The SNPs markers provided by the present invention are all infiltrated into Chinese local pigs by the blood of western pigs, and are all significantly correlated with body length (P<0.01), and can be used for molecular marker-assisted selection breeding of Chinese local pig body length, which can be more effective. Accelerating the speed of breeding and improving the accuracy of breeding has important economic benefits and social value.

The invention discloses a compound PCR amplification system and a detection kit for yak individual identification and paternity identification of the STR marker detection system genotyping. These markers are not linked, have high heterozygosity, rich polymorphic information content, and the size of the amplification product is appropriate; the detection method obtains the genotyping results of 15 STR loci through the amplification and electrophoresis detection of the 15 pairs of primers; According to the typing results of the STR loci and the gene frequency of each STR in the yak, the individual identification and paternity identification of the yak were carried out. The scheme of the present invention can effectively carry out individual identification and paternity identification of yaks by comprehensively analyzing the typing results of STR loci, thereby improving the accuracy of pedigree in yak breeding work, speeding up the breeding process, and has good application prospects and economic value.

The application discloses a SNP marker related to pig birth weight traits and its application. The SNP marker related to the birth weight trait of pigs in this application is located at the 112031589 nucleotide site on chromosome 4 of the pig international genome version 11.1 reference sequence, and has a G or A polymorphism. The SNP markers of the present application related to pig birth weight traits can be used for birth weight prediction of piglets and molecular marker-assisted selection breeding of pigs. Breeding using the SNP markers of the present application can not only shorten the breeding time and improve the accuracy of breeding; it is not easily affected by other external environment and other factors; it can more effectively increase the birth weight of piglets, thereby improving the survival rate, weaning weight and fattening The effect is of great significance to the breeding and production of pigs. Using the SNP marker of the present application for assisted breeding can carry out early selection and timely elimination, reduce breeding costs, quickly obtain homozygous parents, and improve breeding efficiency and quality.

The invention relates to a breeding method for extra-early-maturing sweet potato varieties, which belongs to the technical field of conventional cross-breeding. Sow hybrid seeds with substrate, and 45±5 days after sowing, the height of seedlings reaches 25±5 cm, investigate the tuber formation of seedlings, and select lines with an enlarged diameter of more than 0.8 cm as new lines of selected seed tubers, and then select and breed The new extra-early-maturing sweet potato strain of the present invention has high breeding accuracy, short breeding period and low breeding cost.

The invention relates to the technical field of genetic engineering, in particular to a SNP molecular marker affecting the birth weight of Alpine Merino sheep and its application. The SNP molecular marker is located at the 18805219th nucleotide site C>G mutation on chromosome 14 of the International Sheep Reference Genome Oar_v4.0 version. The present invention also relates to a specific primer pair for detecting the above-mentioned SNP molecular marker using SNaPshot technology, a kit containing the specific primer pair and a detection method for nucleotide polymorphism. Compared with the traditional detection method, the technology has the advantages of accuracy High, fast detection speed, low cost, easy to judge the results and so on. SNP locus detection is used to carry out early selection of alpine merino sheep birth weight traits, shorten the breeding cycle and speed up the breeding process, establish an early selection technology for alpine merino sheep birth weight traits, and reduce the alpine merino sheep birth weight traits. The breeding time of excellent traits reduces the breeding cost and has high application value.

The invention discloses a complete set of reagent and a method for identifying a parent-child relationship of Beijing ducks. The complete set of reagent for identifying or assisting to identify the parent-child relationship of the Beijing ducks is composed of a complete set of reagent 1, a complete set of reagent 2 and a complete set of reagent 3; the complete set of reagent 1 is prepared from IAS05-P, IAS07-P and IAS11-P; the complete set of reagent 2 is prepared from the complete set of reagent 1 and a reagent A; the reagent A is prepared from IAS06-P and IAS08-P; the complete set of reagent 3 is prepared from the complete set of reagent 2 and a reagent B; and the reagent B is prepared from IAS12-P, IAS13-P, IAS04-P, IAS03-P, IAS01-P and IAS14-P. Proved by experiments, the combined exclusion probability of the complete set of reagent of identifying the Beijing ducks can reach 0.99 or above, and the complete set of reagent can be used for complete and accurate pedigree identification and individual recognition of the Beijing ducks and can correct chaotic Beijing duck breeding pedigrees.

The invention relates to molecular genetics, and provides genetic markers for establishing and identifying a dairy cattle molecule system spectrum and application of the genetic markers. The genetic markers consist of 8 loci which are respectively TGLA227, TLA122, BMC1207, BM103, INRA037, INRA134, BP7 and MB026. The invention further provides primers of the 8 microsatellite loci, a method for obtaining fluorescence labeling primers by using the primers to perform fluorescence labeling capillary electrophoresis, a method for performing genotype judgment by using Gene Mapper V3.0 software together with Gene Marker software V1.75, a method for performing genetic relationship identification by using Cervus3.0 software, and a method for establishing an application kit. The genetic markers can be applied to dairy cattle system spectrum identification and establishment, are capable of making important basis for increasing the accuracy of dairy cattle species selection and breeding, are capable of promoting the breeding progress of dairy cattle, and have good application prospects and economical benefits.

The invention relates to a breeding method of a variety of extremely early-maturing sweet potatoes and belongs to the technical field of conventional hybridization breeding. The method comprises the steps of sowing hybrid seeds through a substrate, wherein the height of seedlings reaches 25+ / -5 cm 45+ / -5 days after sowing; investigating the potato setting condition of the seedlings; selecting a strain with the expanded diameter over 0.8 cm as a new strain of selected seedling potatoes; then, breeding the new strain of extremely early-maturing sweet potatoes. The new variety of the extremely early-maturing sweet potatoes has the advantages of high breeding accuracy rate, short breeding period and low breeding cost.

The invention discloses a chloride channel gene fragment related to intramuscular fat deposition of pigs and application thereof. The DNA fragment related to intramuscular fat deposition of pigs is shown in Sequence I of a Sequence Table. The invention also provides a method of applying the DNA fragment to detect the intramuscular fat deposition of pigs in an aided manner. The DNA fragment of theinvention has a very obvious connection with the intramuscular fat deposition of pigs. Compared with the traditional methods, through the detection of pigs at birth by the method of the invention, the intramuscular fat deposition of pigs can be known at the earliest possible time, in addition, early selection can be realized and the breeding selection can be shortened, thus greatly reducing the expenses of the breeding selection. Meanwhile, in the method of the invention, a functional DNA fragment with effects is directly selected, thus improving the accuracy of the breeding selection and reducing the waste of manpower and materials. The invention has very great economic value and social significance.