SNP (single nucleotide polymorphism) molecular marker combination for identifying genetic relationship of Chinese Simmental

A technology of Simmental cattle and kinship, which is applied in the field of SNP molecular marker combination for the identification of kinship of Simmental cattle in China, can solve problems such as difficulties in the quality traceability system and lack of pedigree records

Inactive Publication Date: 2015-05-27
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

The lack of pedigree records has caused great difficulties in promoting the quality traceability system of Simmental beef in China; in addition, pedigree information is needed in the genetic research of cattle

Method used

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  • SNP (single nucleotide polymorphism) molecular marker combination for identifying genetic relationship of Chinese Simmental
  • SNP (single nucleotide polymorphism) molecular marker combination for identifying genetic relationship of Chinese Simmental
  • SNP (single nucleotide polymorphism) molecular marker combination for identifying genetic relationship of Chinese Simmental

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Experimental program
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Embodiment 1

[0067] Example 1 Screening of 50 SNP Molecular Markers for Chinese Simmental Genetic Relationship

[0068] 1. Screening of SNP markers related to the genetic relationship of Chinese Simmental cattle

[0069] The SNP markers of 938 Chinese Simmental cattle were typed using the BovineSNP50 Genotyping Bead Chip chip (purchased from Illumina). Refer to USDA ( http: / / cgemm. / ouisville.edu / usda / index.html ) Screening criteria for SNP markers, screening for SNP markers: ① located on autosomes; ② minimum allele frequency (MAF) greater than 0.35; ③ detection rate (Call rate) of each SNP marker greater than 0.95; ④ the same The distance between adjacent SNPs on a chromosome is greater than 8Mb; in order to avoid linkage disequilibrium among the selected SNP markers, the screening was performed on chromosomes. After preliminary screening, 311 polymorphic SNP sites were obtained. The number distribution of SNP sites on each chromosome is shown in Table 1.

[0070] Table 1 The number of SN...

Embodiment 2

[0083] Example 2 Application of 50 SNP Molecular Markers in Identification of Genetic Relationship of Chinese Simmental Cattle

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Abstract

The invention provides an SNP (single nucleotide polymorphism) molecular marker combination for identifying the genetic relationship of Chinese Simmental. The marker combination contains 50 SNP markers distributed on 25 autosomes, wherein the average distance between adjacent SNP markers on the same autosome is 27M. The average values of the minor allele frequency (MAF), expected heterozygosity (HExp) and polymorphism information content (PIC) of the marker combination are 0.4818, 0.4998 and 0.3748 respectively; and when the genotype of the female parent is unknown, the accumulative exclusion probability is 0.9989. The SNP marker combination provided by the invention can be applied to complete and accurate pedigree identification of the Chinese Simmental, can improve the breeding accuracy of the Chinese Simmental and accelerate the genetic progress, and has good application prospect and economic benefit.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a combination of SNP molecular markers for identification of Chinese Simmental cattle kinship and application thereof. Background technique [0002] Pedigree information plays an important role in cattle breeding. Wrong pedigree will greatly reduce the accuracy of genetic evaluation, affect the effect of selection and matching, reduce the genetic progress of the population, and bring huge economic impact to the cattle industry. However, in actual breeding production, errors in pedigree records are unavoidable, which are affected by many factors, such as breeding records, calving records, human errors in pedigree entry and sorting, etc., especially in extensive free-range pastures After the calf is born, it is difficult to determine the father or even the parents, which often causes pedigree errors. The average pedigree error rate of cattle herds around the world can reach 11% (B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 高雪李俊雅郭立平张路培高会江陈燕
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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