Western pig consanguinity infiltration site SNP marker related to body length in Chinese local pigs, and application of SNP marker

A technology of local pigs and western pigs, applied in the field of marker-assisted selection (MAS) of pigs, which can solve the problems of shortness and lack of dominant body length, and achieve the effect of speeding up the breeding speed and improving the breeding accuracy.

Active Publication Date: 2020-12-18
HENAN AGRICULTURAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a kind of SNP marker of Western pig blood introgression site related to body length in Chinese local pigs, and apply it in genotype identification, genetic breeding and other aspects to solve the problem of Chinese local pigs. Body length is not dominant, generally relatively short

Method used

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  • Western pig consanguinity infiltration site SNP marker related to body length in Chinese local pigs, and application of SNP marker
  • Western pig consanguinity infiltration site SNP marker related to body length in Chinese local pigs, and application of SNP marker
  • Western pig consanguinity infiltration site SNP marker related to body length in Chinese local pigs, and application of SNP marker

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Experimental program
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Embodiment 1

[0049] Embodiment 1: Acquisition of SNP markers

[0050]Using the SNP microarray scan data of 929 heads of 40 Chinese local pig breeds and 165 heads of 6 western pig breeds (878 individuals downloaded from the public database and 216 individuals tested by the applicant), ADMIXTURE population genetic structure analysis was performed. The results show that 8 of them are representative pig breeds of local pig breeds in China: Erhualian pig and Meishan pig representing the Jianghai type, Luchuan pig and Wuzhishan pig representing the South China type, and Ganxi two-headed black pig representing the Central China type. Pigs and Shaziling pigs, Neijiang pigs and Lantang Zang pigs representing the southwest type, and Queshan black pigs are traditionally classified as Central China pig breeds, but they have been largely influenced by the infiltration of Western pig breeds.

[0051] Therefore, in order to obtain the introgression signal of western pig breeds to Chinese local pig breeds...

Embodiment 2

[0052] Example 2: Cloning and Genotype Verification of SNP Markers

[0053] 1. Source of test animals

[0054] Sujiang Pig Breeding Farm in Jiangsu Province: The tested pig breed is Sujiang pig, which is a lean lean bred by Chinese local breed Jiang Quhai and western pig breed Duroc. Meat-type black female breed. Sujiang pigs not only have the advantages of fast growth and high carcass lean meat rate of Western pigs, but also have the characteristics of high fecundity and excellent meat quality of Chinese local pigs. Therefore, Sujiang pig is suitable for evaluating the effect of gene exchange between Chinese and Western pig breeds.

[0055] 2. Extraction of Sujiang pig genomic DNA

[0056] Tissue samples of 365 Sujiang pigs were collected and DNA was extracted; DNA was extracted from pig ear tissue using TIANGEN TIANamp Genomic DNA Kit (DP304-03), and the operation steps were as follows according to the instructions:

[0057] ① First add 68mL and 200mL absolute ethanol to...

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Abstract

The invention relates to a western pig consanguinity infiltration site SNP marker related to the body length in Chinese local pigs, and an application of the SNP marker. The marker is at least one ofthe following mutations: the 2096738th base (rs81439116) of pig chromosome 12 of an international pig genome 11.1 version reference sequence is mutated from A to G, the 2129234th base (rs81439242) ismutated from C to T, and the 2145858th base (rs81439307) is mutated from T to C. The SNPs marker provided by the invention is infiltrated into the Chinese local pigs from the western pig consanguinity, is remarkably associated with the body length (P is less than 0.01), can be used for the molecular marker-assisted selective breeding of the body length of the Chinese local pigs, can more effectively accelerate the selective breeding speed and improve the selective breeding accuracy, and has important economic benefits and social values.

Description

technical field [0001] The invention relates to the technical field of marker-assisted selection (MAS) in pigs, in particular to a SNP marker of a Western pig blood-related infiltration site related to body length in Chinese local pigs and its application. Background technique [0002] my country is one of the earliest domesticated pigs in the world. Various climates and complex terrains across the country have formed a large number of excellent local pig breeds, and 1 / 3 of the local pig breeds in the world come from my country. Local pig breeds in my country are famous for their delicious meat, good motherhood, tolerance to rough feeding, and strong disease resistance, but they generally have the disadvantages of thick backfat and low lean meat percentage. In 2012, the Ministry of Agriculture of my country released the "13th Five-Year Plan for National Livestock Genetic Resources Protection and Utilization" data showing that there are a total of 90 local breeds in my count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q1/6858C12Q2531/113Y02P60/87
Inventor 乔瑞敏徐盼王小女张晨聚明明李明李新建
Owner HENAN AGRICULTURAL UNIVERSITY
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