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A kind of duck egg shell color-related molecular marker and its application

A technology of molecular markers and duck eggs, which is applied in the direction of biochemical equipment and methods, DNA/RNA fragments, microbial measurement/inspection, etc., can solve the problems of cumbersome breeding process, long breeding cycle, and high breeding cost, so as to improve breeding efficiency, The effect of low cost and easy implementation

Active Publication Date: 2022-05-10
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the defects of cumbersome breeding process, long breeding cycle, multi-generation continuous breeding, and high breeding cost.

Method used

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  • A kind of duck egg shell color-related molecular marker and its application
  • A kind of duck egg shell color-related molecular marker and its application
  • A kind of duck egg shell color-related molecular marker and its application

Examples

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Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Discovery of Molecular Markers

[0031] A total of 16,283,527 SNPs were obtained through genome-wide resequencing of 6 homozygous green-shelled ducks, 10 heterozygous green-shelled ducks, and 10 homozygous white-shelled ducks, of which 7,179,415 SNPs were used for genome-wide association analysis. . It was found that 3491669 to 3603975 on KB742619.1 were significantly correlated with the green shell phenotype of duck eggs (P-6 ). The genotype-haplotype analysis of the above regions revealed that there were 7 SNP locus genotypes consistent with the sequenced individual genotypes. Experimental verification and expanded population genotype verification were carried out on these 7 SNP sites, and it was found that the 1157th site (G1157A) of the sequence shown in SEQ ID NO. The causative locus can be used to determine the color of duck eggs by detecting its genotype.

[0032] 1. Collection of duck blood samples and DNA extraction

[0033] The Shaoxing duck use...

Embodiment 2

[0051] Embodiment 2: Genotype-phenotype correlation verification of G1157A locus in different populations

[0052] The Shaoxing duck, Shanshan duck and Youxian duck used in the experiment came from Zhuji Guowei Poultry Industry Co., Ltd.; the Peking duck came from Huzhou Zhuowang Agricultural Technology Company; the mallard duck came from Fenghua Aoji Poultry Professional Cooperative.

[0053] A total of 1328 individual blood samples were collected from six species of Shaoxing duck, Shanma duck, Jinyun shelduck, Peking duck, Youxian shelduck and mallard duck, and the genotype analysis of duck eggshell color molecular markers was carried out using the method described in Example 1 .

[0054] After the above-mentioned blood collection individuals lay eggs, record and count the color of their eggs. Table 1 shows the results of correlation analysis between genotypes at the G1157A locus genotype and eggshell color of duck eggshell color in different breeds. It can be seen from th...

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Abstract

The invention belongs to the field of poultry molecular breeding, and relates to a duck egg shell color molecular marker and its application in duck egg shell color prediction and egg-type breeding duck selection. The nucleotide sequence of the molecular marker of the present invention is as shown in SEQ ID NO.1 or SEQ ID NO.2, and the change of the genotype of the above-mentioned molecular marker G1157A site can be analyzed by PCR and sequencing methods, which can be accurate, fast and effective The eggshell color prediction of duck eggs provides a powerful molecular biological basis and implementation method for duck breeding.

Description

technical field [0001] The invention belongs to the fields of poultry breeding and molecular biology. Specifically, it relates to a duck egg shell color-related molecular marker and its application in duck egg shell color prediction and breeding duck breeding. Background technique [0002] The eggshells of poultry are mainly divided into three types according to their colors: brown shells, green shells, and white shells, among which brown shells and white shells are the two main eggshell colors. The eggshell color of poultry is one of the important characteristics of breeds and strains just like its feathers and plumage color, and has become an important content in the selection of breeds and strains in the breeding process. [0003] Eggshell color is one of the indicators to measure egg quality. Compared with the other two colors, it is generally believed that green-shelled eggs have thicker eggshells, higher eggshell strength, and stronger resistance to solar radiation, o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 陈黎卢立志李宁胡晓湘顾晓荣黄学涛李国勤沈军达陶争荣田勇曾涛徐坚
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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