Genetic marker for establishing and identifying dairy cattle molecule system spectrum and application of genetic marker

A genetic marker and molecular pedigree technology, which is applied in the determination/examination of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. , to achieve good application prospects and economic benefits, high detection efficiency, and optimized detection costs.
CN106222292AActive Publication Date: 2016-12-14SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG AGRICULTURAL UNIVERSITY
Publication Date
2016-12-14

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Abstract

The invention relates to molecular genetics, and provides genetic markers for establishing and identifying a dairy cattle molecule system spectrum and application of the genetic markers. The genetic markers consist of 8 loci which are respectively TGLA227, TLA122, BMC1207, BM103, INRA037, INRA134, BP7 and MB026. The invention further provides primers of the 8 microsatellite loci, a method for obtaining fluorescence labeling primers by using the primers to perform fluorescence labeling capillary electrophoresis, a method for performing genotype judgment by using Gene Mapper V3.0 software together with Gene Marker software V1.75, a method for performing genetic relationship identification by using Cervus3.0 software, and a method for establishing an application kit. The genetic markers can be applied to dairy cattle system spectrum identification and establishment, are capable of making important basis for increasing the accuracy of dairy cattle species selection and breeding, are capable of promoting the breeding progress of dairy cattle, and have good application prospects and economical benefits.
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Description

technical field

[0001] The invention relates to molecular genetics, and provides a group of genetic markers for constructing and identifying dairy cow molecular pedigrees and applications thereof. Background technique

[0002] The genetic improvement of dairy cows requires the determination of the offspring of breeding bulls and molecular marker-assisted selection of dairy cows, and the accuracy of pedigree records is crucial to the genetic improvement of dairy cows. The lack of strict management of some small and medium-sized dairy farms leads to the lack of dairy cow pedigree, or due to the popularization of artificial insemination technology and inevitable errors in the production process (pedigree record), resulting in incomplete or wrong pedigree records of cow herds, so the molecular pedigree construction and pedigree of dairy cows Accuracy assessment has become an important part of genetic improvement.

[0003] Microsatellite marker technology is a relatively mature ...

Claims

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