Kit and method for family division and paternity identification of domestic pigs

A paternity test and kit technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as division and few paternity test reports.
CN113373243AActive Publication Date: 2021-09-10ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANHUI AGRICULTURAL UNIVERSITY
Publication Date
2021-09-10

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Abstract

The invention belongs to the technical field of livestock molecular biology, and provides a kit and method for family division and paternity identification of domestic pigs. The kit and method comprises the following steps: specifically selecting 14 SSR loci of domestic pigs, especially Anqing Liubai pigs, synthesizing corresponding primers for the loci, conducting capillary electrophoresis detection on ear tissue DNA of 98 Anqing Liubai pigs, counting and calculating the number of effective alleles, heterozygosity, polymorphic information content, genetic distance and exclusion rate of each locus, obtaining family division of the domestic pigs, especially the Anqing Liubai pigs, and performing paternity identification. The cumulative elimination probability of 14 microsatellite loci reaches 99%. The 14 microsatellite loci selected by the research have polymorphism in the Anqing Liubai pig population and can be used as effective genetic markers to be applied to production practices such as family division, paternity identification and the like of domestic pigs, especially the Anqing Liubai pig population.
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Description

technical field

[0001] The invention belongs to the technical field of livestock molecular biology, and relates to a pig family division and paternity identification kit and method. Background technique

[0002] The protection of pig breed resources relies on local breed conservation farms. In the production process, there are multiple introductions, unclear records of pedigree and production information, etc., which lead to unclear kinship of pigs in stock and increased risk of inbreeding, which directly leads to high-quality pigs. The number of breeding pig families has decreased, and the purity and genetic diversity of breeds have decreased, seriously affecting the preservation of high-quality breeding pig germplasm resources and industrial development. Therefore, accurate kinship is of great significance for formulating pig breeding plans, avoiding inbreeding, saving seed selection, and speeding up the breeding process.

[0003] Simple Sequence Repeats (Simple Sequence ...

Claims

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