SNP (Single Nucleotide Polymorphism) marker for identifying weaning weight of alpine merino and application of SNP marker

A Merino sheep and alpine technology, applied in the field of molecular genetics, can solve the problems of long breeding cycle and slow genetic progress, and achieve the effects of high accuracy, low cost and fast detection speed

Active Publication Date: 2022-06-24
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Traditional breeding methods mostly use traditional phenotypic breeding techniques, that is, to infer parental productivity based on offspring phenotype information, long breeding cycle, and slow genetic progress

Method used

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  • SNP (Single Nucleotide Polymorphism) marker for identifying weaning weight of alpine merino and application of SNP marker
  • SNP (Single Nucleotide Polymorphism) marker for identifying weaning weight of alpine merino and application of SNP marker
  • SNP (Single Nucleotide Polymorphism) marker for identifying weaning weight of alpine merino and application of SNP marker

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Correlation between different genotypes and weaning weight of Alpine Merino sheep

[0034] 1. Sample Collection

[0035] The samples were from the sheep breeding technology extension station in Gansu Province. Blood samples were collected from 115 Alpine Merino sheep with production records. 5 mL of blood was collected intravenously from each sheep in a blood collection tube with EDTA-K2 anticoagulant. After the blood samples were collected, they were quickly mixed. , put it in a sampling box containing an ice pack for temporary storage, and then transport it back to the laboratory and store it in a -20°C refrigerator for DNA extraction. The weaning weight record of each sheep was provided by Gansu Provincial Sheep Breeding Technology Extension Station.

[0036] 2. Main reagents and instruments

[0037]EDTA-K2 vacuum blood collection tube was purchased from Jiangsu Yuli Medical Instrument Co., Ltd.; blood genome extraction kit was purchased from Tiangen Bioc...

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Abstract

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker for identifying the weaning weight of alpine merino and application of the SNP marker, and the SNP molecular marker is located at the 3566034th nucleotide site C / T mutation on the 23rd chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the weaning weight of the high-mountain merino sheep, shortening the breeding period and accelerating the breeding process, a technology for early selection of the weaning weight of the high-mountain merino sheep is established, the breeding time of excellent characters of the weaning weight of the high-mountain merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

Description

technical field [0001] The invention belongs to the technical field of molecular genetics, and in particular relates to a SNP marker for identifying the weaning weight of Alpine Merino sheep and its application. Background technique [0002] For breeding stock, fecundity is productivity, and reproductive performance is directly related to the economic effect of animal husbandry. The reproductive performance of sheep mainly includes the total number of lambs, the number of live lambs, birth weight, birth litter weight, weaning weight and other indicators. The weight of sheep at birth is the birth weight of sheep, which is an important quantitative trait to measure sheep, which affects the growth and development, production performance and carcass grade of sheep. Birth weight was also positively correlated with weaning survival rate, weaning weight and slaughter weight, and negatively correlated with slaughter age. And sheep with low birth weight are mostly stunted. Therefo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11C12Q1/686
CPCC12Q1/6888C12Q1/686C12Q2600/156C12Q2600/124C12Q2525/161Y02P60/87
Inventor 卢曾奎袁超郭婷婷杨博辉刘建斌岳耀敬李建烨牛春娥
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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