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Microsatellite markers for identifying parent-child relationships of Chinese Simmental cattle and application of microsatellite markers

A technology of Simmental cattle and microsatellite markers, applied in the field of molecular genetics, can solve the problems of high detection cost, time-consuming, low repeatability, etc., and achieve the effect of improving detection efficiency

Inactive Publication Date: 2015-11-11
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in view of the shortcomings of the existing genotyping technology, such as complicated operation, time-consuming, low repeatability, and the existing commercial kits are expensive, and the detection cost is high, through the improvement of the detection technology, the screening Genotype simultaneously achieves the purpose of detection

Method used

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  • Microsatellite markers for identifying parent-child relationships of Chinese Simmental cattle and application of microsatellite markers
  • Microsatellite markers for identifying parent-child relationships of Chinese Simmental cattle and application of microsatellite markers
  • Microsatellite markers for identifying parent-child relationships of Chinese Simmental cattle and application of microsatellite markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Screening and determination of microsatellite markers for paternity identification of Chinese Simmental cattle

[0047] 1. Selection and grouping of microsatellite marker loci

[0048] According to relevant reports of literature research, 14 microsatellite loci with 14 different chromosomes and high polymorphic information content in cattle were selected. The principle of selection is: rich alleles, high polymorphism, located on different chromosomes, no linkage relationship between each other, good repeatability, no "dumb alleles (NullAllele)" and so on. See Table 4 for details.

[0049] Table 414 Basic information of microsatellite loci

[0050]

[0051]

[0052] In order to reduce the detection cost of microsatellite genotyping, PCR products were mixed and grouped according to the fragment length of microsatellite markers and the fluorescent groups carried. In order to avoid non-specific band interference in the same group, the fluorescent colors i...

Embodiment 210

[0071] Application of Example 210 Microsatellite Markers in Identification of Parentage of Chinese Simmental Cattle

[0072] In this example, a father-son pair with a clear father-son relationship and an unrelated individual were selected from the Chinese Simmental cattle parent-child population for verification.

[0073] 1. Sample:

[0074] (1) Blood sample of offspring calf 19105102, No. 1;

[0075] (2) Frozen semen of the candidate male bull cow1171, No. 2;

[0076] (3) Frozen semen of candidate male bull 14_2620, No. 3.

[0077] 2. Identification requirements: DNA test, paternity test

[0078] 3. Inspection and results

[0079] The DNA of the sample to be tested was extracted, and after performing PCR amplification on 10 microsatellite genetic markers, it was detected on an ABI3730 automatic sequencer. The results are shown in Table 9.

[0080] Table 9 test results

[0081] site

number 1

number 2

number 3

[0082] TGLA53

162，...

Embodiment 310

[0085] Application of 310 microsatellite markers in the genetic improvement and breeding of Chinese Simmental cattle

[0086] 1. Sample:

[0087] Bull frozen semen, tube number 10008, sample number 1;

[0088] Bull frozen semen, tube number 20017, sample number 2.

[0089] 2. Identification requirements: DNA test, sample 1 and sample 2 consistency identification

[0090] 3. Inspection and results

[0091] The DNA of the sample to be tested was extracted, and after performing PCR amplification on 10 microsatellite genetic markers, it was tested on an ABI3730 automatic sequencer. The results are shown in Table 10.

[0092] Table 10 test results

[0093] site

number 1

number 2

TGLA53

162，166

162，166

BM2113

129，129

129，129

[0094] INRA023

206，207

206，207

TGLA122

150，154

150，154

CSSM66

188，199

188，199

ETH225

147，147

147，147

SPS115

333，337

333，337 ...

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Abstract

The invention provides a group of microsatellite markers for identifying parent-child relationships of Chinese Simmental cattle. The group of microsatellite markers includes ten microsatellite markers. The microsatellite markers have the advantages that the markers are high in polymorphism and are not linked with one another, fragments are appropriate in size and interval, and simultaneous detection can be facilitated; combined exclusion probabilities CPE (1), CPE (2) and CPE (3) can respectively reach 0.9960969, 0.999854 and 0.9999997 when the parent-child relationships of the Chinese Simmental cattle are identified by the aid of marker combinations under three different conditions, and accordingly the microsatellite markers are extremely high in detection efficiency; the microsatellite marker combinations can be used for completely and accurately identifying family trees of the Chinese Simmental cattle, accordingly, the Chinese Simmental cattle breeding accuracy can be improved, breeding procedures can be accelerated, and the microsatellite markers have excellent application prospects and economic benefits.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and in particular relates to a microsatellite marker for identification of parentage of Chinese Simmental cattle and its application. Background technique [0002] Pedigree information plays an important role in cattle breeding. Wrong pedigree will greatly reduce the accuracy of genetic evaluation, affect the effect of selection and matching, reduce the genetic progress of the population, and bring huge economic impact to the cattle industry. However, in actual breeding production, pedigree record errors are unavoidable, and it is affected by many factors, such as breeding records, calving records, human errors in pedigree entry and sorting, etc., especially in extensive free-range pastures. After the calf is born, it is difficult to determine the father or even the parents, which often leads to pedigree errors. [0003] At present, microsatellite marker method is the most researched and appli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 高雪李俊雅孙东晓贺建宁宋玲郭立平
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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