SNP molecular marker combination for establishing Luxi cattle pedigree

A molecular marker, Luxi cattle technology, applied in the field of molecular genetics, can solve the problems of lack of pedigree records, difficulty of quality traceability system, etc.

Inactive Publication Date: 2019-11-01
INNER MONGOLIA UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lack of pedigree records has caused great difficulties in promoting the quality traceability system of Luxi beef. In addition, pedigree information is needed in the genetic research of cattle

Method used

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  • SNP molecular marker combination for establishing Luxi cattle pedigree
  • SNP molecular marker combination for establishing Luxi cattle pedigree
  • SNP molecular marker combination for establishing Luxi cattle pedigree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Screening of 100 SNP molecular markers of Luxi cattle genetic relationship

[0068]Using the Bovine SNP150K Genotyping Bead Chip chip (purchased from Illumina) to analyze the results and data, the markers of XX Luxi cattle were genotyped. Refer to the US Department of Agriculture's screening criteria for SNP markers, and screen markers ① located on autosomes ② minimum allele frequency (MAF) greater than 0.35 ③ detection rate of each marker (Callrate) greater than 0.95 ④ similar on the same chromosome Neighbor distance greater than 8Mb; in order to avoid linkage disequilibrium among the selected SNP markers, separate chromosomes for screening. After preliminary screening, 701 polymorphic sites were obtained, and the number distribution of the sites on each chromosome is shown in Table 3.

[0069] chromosome SNPs average distance 1 49 7993223 2 38 7073627 3 39 8480873 4 40 7457215 5 30 7422463 6 29 6793028 7...

Embodiment 2

[0080] Example 2 Application of 100 Molecular Markers in Identification of Genetic Relationship of Luxi Cattle

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Abstract

The invention provides an SNP molecular marker combination for establishing a Luxi cattle pedigree. The SNP molecular marker combination comprises 100 SNP markers distributed on 29 autosomes, the average distance of adjacent SNP markers on the same chromosome is 24M. Mean values of minor allele frequency (MAF), expected heterozygosity (HExp) and polymorphic information content (PIC) of the markercombination are respectively 0.4836, 0.4997 and 0.3751; and the accumulative probability of exclusion is 0.9991. The SNP marker combination can be used for completely and accurately identifying the pedigree of Luxi cattle, can improve the accuracy of Luxi cattle breeding and accelerating the genetic progress of the Luxi cattle, and has an excellent application prospect and economic benefit.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a combination of SNP molecular markers for establishment and identification of Luxi cattle pedigree and its application. Background technique [0002] Pedigree information plays an important role in cattle breeding. Wrong pedigree will greatly reduce the accuracy of genetic evaluation, affect the effect of selection and matching, reduce the genetic progress of the population, and bring huge economic impact to the cattle industry. However, in actual breeding production, errors in pedigree records are unavoidable, which are affected by many factors, such as breeding records, calving records, human errors in pedigree entry and sorting, etc., especially in extensive free-range pastures After the calf is born, it is difficult to determine the father or even the parents, which often causes pedigree errors. The average pedigree error rate of cattle herds in all countries in the world c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 佟彬李光鹏赵云博李亚星李庆东王洪
Owner INNER MONGOLIA UNIVERSITY
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