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A complete set of reagents and methods for identifying the parent-child relationship of Peking ducks

A parent-child relationship, Peking duck technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as inability to use parent-child testing, lack of microsatellite marker system, and lack of highly polymorphic microsatellite sequences.

Active Publication Date: 2018-02-23
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microsatellite marker system for duck paternity testing is still vacant
At present, the NCBI website only reports hundreds of duck microsatellite markers, and most of these markers cannot be used for paternity identification due to their poor stability and low polymorphism. morphological microsatellite sequence and its lack of

Method used

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  • A complete set of reagents and methods for identifying the parent-child relationship of Peking ducks
  • A complete set of reagents and methods for identifying the parent-child relationship of Peking ducks
  • A complete set of reagents and methods for identifying the parent-child relationship of Peking ducks

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0217] Example 1. A complete set of reagents for identifying or assisting in identifying the paternity of Peking ducks can be used to identify the paternity of Peking ducks

[0218] 1. Preparation of a complete set of reagents for identifying or assisting in identifying the parent-child relationship of Peking ducks

[0219] The set of reagents provided in this example for identifying or assisting in the identification of the parent-child relationship of Peking ducks is the set of reagents 1, the set of reagents 2 and the set of reagents 3;

[0220] Set of reagents 1 consists of primer pairs named IAS05-P, IAS07-P and IAS11-P; IAS05-P consists of single-stranded DNA named IAS05-F and IAS05-R; IAS07-P consists of primers named IAS07-F and IAS07-R consist of single-stranded DNA; IAS11-P consists of single-stranded DNA named IAS11-F and IAS11-R, respectively;

[0221] Set of reagents 2 consists of set of reagents 1 and reagent A; reagent A consists of primer pairs named IAS06-P a...

Embodiment 2

[0271] Example 2. Application of a complete set of reagents for identifying or assisting in identifying the parent-child relationship of Peking ducks in the identification of parent-child relationship of Peking ducks

[0272] Three Peking ducks were selected from the Beijing Duck Breeding Farm of the Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, numbered E03, E07 and C12. ), the Peking duck numbered E03 has no relationship with E07 (that is, the unrelated father). Next, E03 and C12 are used as candidate fathers of E07 to verify that the set of reagents in Example 1 can be used to identify the parent-child relationship of Peking ducks.

[0273] Extract the genomic DNA of the above three Peking ducks respectively, use the complete set of reagents 3 of Example 1 to carry out PCR amplification according to the method of Example 1, detect on the ABI PRISM3730XL genetic analyzer, and determine the genotype of each microsatel...

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Abstract

The invention discloses a complete set of reagent and a method for identifying a parent-child relationship of Beijing ducks. The complete set of reagent for identifying or assisting to identify the parent-child relationship of the Beijing ducks is composed of a complete set of reagent 1, a complete set of reagent 2 and a complete set of reagent 3; the complete set of reagent 1 is prepared from IAS05-P, IAS07-P and IAS11-P; the complete set of reagent 2 is prepared from the complete set of reagent 1 and a reagent A; the reagent A is prepared from IAS06-P and IAS08-P; the complete set of reagent 3 is prepared from the complete set of reagent 2 and a reagent B; and the reagent B is prepared from IAS12-P, IAS13-P, IAS04-P, IAS03-P, IAS01-P and IAS14-P. Proved by experiments, the combined exclusion probability of the complete set of reagent of identifying the Beijing ducks can reach 0.99 or above, and the complete set of reagent can be used for complete and accurate pedigree identification and individual recognition of the Beijing ducks and can correct chaotic Beijing duck breeding pedigrees.

Description

technical field [0001] The invention relates to a set of reagents and a method for identifying the parent-child relationship of Peking ducks in the field of biotechnology. Background technique [0002] China is one of the first countries in the world to domesticate and raise wild ducks. Duck meat and its processed by-products such as duck neck, duck intestines, and duck feet have a very broad mass base in China. Peking duck is a breed selected and bred by the Waterfowl Research Office of Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences after decades. [0003] Accurate pedigree information is the basis for the smooth development of poultry genetic breeding and the fundamental guarantee for genetic progress. Wrong pedigree information will affect the accuracy of genetic evaluation, reduce genetic progress, and bring huge economic losses to breeding farms and production enterprises. In the actual breeding work, all links ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156
Inventor 侯水生周正奎凡文磊程红
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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