Anti-WSSV (white spot syndrome virus) candidate molecular marker from anti-lipopolysaccharide factor gene (nLvALF6) of litopenaeus vannamei and application of anti-WSSV candidate molecular marker
An anti-lipopolysaccharide factor and molecular marker technology, applied in recombinant DNA technology, microbial assay/test, DNA/RNA fragments, etc. The effect of avoiding breeding errors and improving efficiency
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[0023] Acquisition of SNP markers related to shrimp resistance to WSSV
[0024] 1. Acquisition of WSSV susceptible and resistant materials
[0025] WSSV susceptible and resistant materials come from the mixed population of "Kehai No. 1" multi-lineage. 500 healthy Litopenaeus vannamei were selected and placed in 12 50L aquariums. Before the experiment, 10 prawns were randomly selected to detect no WSSV infection. The experimental water temperature was (23±1)℃. After the starvation treatment, the diseased shrimps caused by WSSV infection were fed (WSSV virus content was 10 4 -10 6 copy / ng DNA). After challenge, check 3 times a day (each interval 6h) and collect dead shrimp, randomly sample and extract genomic DNA, the earliest dead 48 prawns were used as WSSV susceptibility group (susceptible group, sus). On the 14th day after challenge, the survival of Litopenaeus vannamei was stable, and 48 surviving prawns were selected as the WSSV resistant group (res).
[0026] 2. Geno...
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