Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of rs81439242 SNP molecular marker in breeding of live pig varieties related to body length

A technology of molecular markers and live pigs, which is applied in the directions of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of pig body length not dominant, short and small, etc., so as to improve the accuracy of breeding and speed up the selection The effect of breeding speed

Active Publication Date: 2022-01-14
HENAN AGRICULTURAL UNIVERSITY +1
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to apply the rs81439242 SNP molecular marker to the selection and breeding of pig strains related to body length, such as applying it in genotype identification, genetic breeding, etc., to solve the problem that the body length of local pigs in China is not dominant. relatively short question

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of rs81439242 SNP molecular marker in breeding of live pig varieties related to body length
  • Application of rs81439242 SNP molecular marker in breeding of live pig varieties related to body length
  • Application of rs81439242 SNP molecular marker in breeding of live pig varieties related to body length

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: Acquisition of SNP markers

[0050] Using the SNP chip scanning data of 929 heads of 40 Chinese local pig breeds and 165 heads of 6 western pig breeds (878 individuals downloaded from the public database and 216 individuals detected by the inventor of the present application), ADMIXTURE population genetic structure analysis was carried out The results show that 8 of them are representative pig breeds of local pig breeds in China: Erhualian pig and Meishan pig representing the Jianghai type, Luchuan pig and Wuzhishan pig representing the South China type, and two Jiangxi pigs representing the Central China type Wu pigs and Shaziling pigs, Neijiang pigs and Lantang Zang pigs representing the southwest type, and Queshan black pigs are traditionally classified as Huazhong pig breeds, but they are largely influenced by the infiltration of Western pig breeds.

[0051] Therefore, in order to obtain the introgression signal of western pig breeds to Chinese local p...

Embodiment 2

[0052] Example 2: Cloning and Genotype Verification of SNP Markers

[0053] 1. Source of test animals

[0054] A Sujiang pig breeding farm in Jiangsu Province: The tested pig breed is Sujiang pig, which is bred by the Chinese local pig breed Jiang Quhai and the western pig breed Duroc by cross-fixation and subbreeding Lean black female breed. Sujiang pigs not only have the advantages of fast growth and high carcass lean meat rate of Western pigs, but also have the characteristics of high fecundity and excellent meat quality of Chinese local pigs. Therefore, Sujiang pig is suitable for evaluating the effect of gene exchange between Chinese and Western pig breeds.

[0055] 2. Extraction of Sujiang pig genomic DNA

[0056] Tissue samples of 365 Sujiang pigs were collected and DNA was extracted; the pig ear tissue DNA was extracted using TIANGEN TIANamp Genomic DNA Kit (DP304-03), and the operation steps were implemented as follows according to the instructions:

[0057] ①Add ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of an rs81439242 SNP molecular marker in breeding of live pig varieties related to body length. The rs81439242 SNP marker integrates into Chinese local pigs from western pig consanguinity, is remarkably associated with the body length (P<0.01), and can be used for molecular marker-assisted selective breeding of Chinese local pigs related to body length, thereby accelerating breeding speed more effectively and improving breeding accuracy. The rs81439242 SNP marker has important economic benefits and social application values.

Description

[0001] The patent application of the present invention is a divisional application with the application number 202010956915.5, the application date is September 12, 2020, and the invention title is "SNP markers and applications of Western pig blood-related infiltration sites related to body length in Chinese local pigs". technical field [0002] The invention relates to the technical field of pig marker-assisted selection (MAS), in particular to the application of a rs81439242 SNP molecular marker in the breeding of pig strains related to body length. Background technique [0003] my country is one of the earliest domesticated pigs in the world. Various climates and complex terrains across the country have formed a large number of excellent local pig breeds, and 1 / 3 of the local pig breeds in the world come from my country. Local pig breeds in my country are famous for their delicious meat, good motherhood, tolerance to rough feeding, and strong disease resistance, but they ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q1/6858C12Q2531/113Y02P60/87
Inventor 乔瑞敏徐盼王小女张晨聚明明李明李新建
Owner HENAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com